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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/01/2018 |
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Data da última atualização: |
27/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; QUINTAO, C. C. R.; FREITAS, C. de; CAMARGO, A. J. R.; SERAPIAO, R. V.; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; CELIO DE FREITAS, CNPGL; Agostinho Jorge dos Reis Camargo, PESAGRO RIO; Raquel Varella Serapião, PESAGRO RIO; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Post implantation development reveals that biopsy procedure can segregate "healthy" from "unhealthy" bovine embryos and prevent miscarriages. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reproduction Science, v. 184, p. 51-58, 2017. |
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DOI: |
10.1016/j.anireprosci.2017.06.016 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73.7%; expanded blastocyst: 73.1%), but affects (p<0.05) implantation (regular blastocyst: 37.8%, expanded blastocyst: 61.0%), so ideally biopsy should be performed in expanded blastocysts. No detrimental effect of biopsy procedure was detected for post-implantation development (calving rates, Biopsy: 47.1%, Control: 41.9%), and normal calves were born (Birth weight, Biopsy: 32.10±7.20kg; Control: 30.95±5.43kg). Surprisingly, we found interesting results suggesting embryo survival can be increased with aggressive procedures (such as embryo biopsy), and this is highly associated with early pregnancy loss (Biopsy: 0%, Control: 17.4%). This finding also suggests morphological classification of day 7 blastocysts is far from ideal, and supposedly, unhealthy embryos can implant but are bound to miscarriage during the first trimester (non-biopsied embryos). Our results show biopsy procedure is safe for bovine IVF embryos, and shed new light into the importance of conceptus in early pregnancy loss in cattle. MenosAbstract Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73.7%; expanded blastocyst: 73.1%), but affects (p<0.05) implantation (regular blastocyst: 37.8%, expanded blastocyst: 61.0%), so ideally biopsy should be performed in expanded blastocysts. No detrimental effect of biopsy procedure was detected for post-implantation development (calving rates, Biopsy: 47.1%, Control: 41.9%), and normal calves were born (Birth weight, Biopsy: 32.10±7.20kg; Control: 30.95±5.43kg). Surprisingly, we found interesting results suggesting embryo survival can be increased with aggressive procedures (such as embryo biopsy), and this is highly associated with early pregnancy loss (Biopsy: 0%, Control: 17.4%). This finding also suggests morphological classification of day 7 blastocysts is far from ideal, and supposedly, unhealthy embryos can implant but are bound to miscarriage during the first trimester (non-biopsied embryos). Our results show b... Mostrar Tudo |
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Palavras-Chave: |
Bovine; Early pregnancy loss; Embryo biopsy; Genomic selection; Post-implantation development. |
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Thesaurus Nal: |
in vitro fertilization. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02511naa a2200265 a 4500
001 2085096
005 2023-01-27
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1016/j.anireprosci.2017.06.016$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aPost implantation development reveals that biopsy procedure can segregate "healthy" from "unhealthy" bovine embryos and prevent miscarriages.$h[electronic resource]
260 $c2017
520 $aAbstract Embryo biopsy has been performed in bovine in vivo produced embryos for the last twenty years, but little could be done with few embryonic cells in the past. Recently, advances in single cell analysis enabled a wide range of applications using embryo biopsy, from morphology to genetics analysis and different omics-techniques, which are promising for in vitro-fertilized (IVF) embryos. The aim of this study was to address if biopsy procedure would affect post implantation development of IVF blastocyts. Here we show that blastocyst stage do not affect re-expansion of biopsied embryos (regular blastocyst: 73.7%; expanded blastocyst: 73.1%), but affects (p<0.05) implantation (regular blastocyst: 37.8%, expanded blastocyst: 61.0%), so ideally biopsy should be performed in expanded blastocysts. No detrimental effect of biopsy procedure was detected for post-implantation development (calving rates, Biopsy: 47.1%, Control: 41.9%), and normal calves were born (Birth weight, Biopsy: 32.10±7.20kg; Control: 30.95±5.43kg). Surprisingly, we found interesting results suggesting embryo survival can be increased with aggressive procedures (such as embryo biopsy), and this is highly associated with early pregnancy loss (Biopsy: 0%, Control: 17.4%). This finding also suggests morphological classification of day 7 blastocysts is far from ideal, and supposedly, unhealthy embryos can implant but are bound to miscarriage during the first trimester (non-biopsied embryos). Our results show biopsy procedure is safe for bovine IVF embryos, and shed new light into the importance of conceptus in early pregnancy loss in cattle.
650 $ain vitro fertilization
653 $aBovine
653 $aEarly pregnancy loss
653 $aEmbryo biopsy
653 $aGenomic selection
653 $aPost-implantation development
700 1 $aQUINTAO, C. C. R.
700 1 $aFREITAS, C. de
700 1 $aCAMARGO, A. J. R.
700 1 $aSERAPIAO, R. V.
700 1 $aCAMARGO, L. S. de A.
773 $tAnimal Reproduction Science$gv. 184, p. 51-58, 2017.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
12/11/2007 |
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Data da última atualização: |
11/08/2011 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BARROS, A. L. de; SILVA, F. V. da; NOGUEIRA, A. R. de A. |
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Afiliação: |
Allen Lopes de Barros - IQSC/USP; Fernando Vitorianao da Silva - Varian Indústria e Comércio Ltda; ANA RITA DE ARAUJO NOGUEIRA, CPPSE. |
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Título: |
Emprego de sistema em fluxo a altas temperatura e pressão no preparo de amostras de suco. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
In: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 14., 2007, João Pessoa, PB. Anais... João Pessoa: SBQA, 2007. |
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Descrição Física: |
1 CD-ROM |
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Idioma: |
Português |
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Palavras-Chave: |
Preparo de amostra; Sistema de fluxo. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/47932/1/PROCIARAN2007.00201.pdf
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Marc: |
LEADER 00550nam a2200157 a 4500
001 1047932
005 2011-08-11
008 2007 bl uuuu u00u1 u #d
100 1 $aBARROS, A. L. de
245 $aEmprego de sistema em fluxo a altas temperatura e pressão no preparo de amostras de suco.
260 $aIn: ENCONTRO NACIONAL DE QUÍMICA ANALÍTICA, 14., 2007, João Pessoa, PB. Anais... João Pessoa: SBQA$c2007
300 $c1 CD-ROM
653 $aPreparo de amostra
653 $aSistema de fluxo
700 1 $aSILVA, F. V. da
700 1 $aNOGUEIRA, A. R. de A.
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Embrapa Pecuária Sudeste (CPPSE) |
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