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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
27/12/2017 |
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Data da última atualização: |
03/01/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
POLESE, V.; SOARES, C. de P.; SILVA, P. R. A. da; ARAUJO, J. L. S. de; BALDANI, J. I.; VIDAL, M. S. |
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Afiliação: |
VALÉRIA POLESE, UFRRJ; LEITON DE PAULA SOARES, UFRRJ; PAULA RENATA ALVES DA SILVA, UFRRJ; JEAN LUIZ SIMOES DE ARAUJO, CNPAB; JOSE IVO BALDANI, CNPAB; MARCIA SOARES VIDAL, CNPAB. |
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Título: |
Selection and validation of reference genes for RT-qPCR indicates that juice of sugarcane varieties modulate the expression of C metabolism genes in the endophytic diazotrophic Herbaspirillum rubrisubalbicans strain HCC103. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Antonie van Leeuwenhoekv, 110, n. 12, p. 1555-1568, 2017. |
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ISSN: |
1572-9699 |
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DOI: |
https://doi.org/10.1007/s10482-017-0906-7 |
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Idioma: |
Inglês |
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Conteúdo: |
Quantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Together the results suggest that dnaG and gyrA genes are suitable as reference genes for RT-qPCR analysis of strain HCC103 and that juice from different sugarcane varieties modulates the expression of key genes involved in carbon metabolism. MenosQuantitative reverse transcription PCR (RT-qPCR) is an important tool for evaluating gene expression. However, this technique requires that specific internal normalizing genes be identified for different experimental conditions. To date, no internal normalizing genes are available for validation of data analyses for Herbaspirillum rubrisubalbicans strain HCC103, an endophyte that is part of the sugarcane consortium inoculant. This work seeks to identify and evaluate suitable reference genes for gene expression studies in HCC103 grown until middle log phase in sugarcane juice obtained from four sugarcane varieties or media with three different carbon sources. The mRNA levels of five candidate genes (rpoA, gyrA, dnaG, recA and gmK) and seven target genes involved in carbon metabolism (acnA, fbp, galE, suhB, wcaA, ORF_0127.0101 and _0127.0123) were quantified by RT-qPCR. Analysis of expression stability of these genes was carried out using geNorm and Normfinder software. The results indicated that the HCC103 dnaG and gyrA genes are the most stable and showed adequate relative expression level changes among the different sugarcane juices. The highest expression level was seen for ORF_0127.0101, which encodes a sugar transporter, in juice from sugarcane variety RB867515 and glucose as the carbon source. The suhB gene, encoding SuhB inositol monophosphatase, had a higher relative expression level on 0.5% glucose, 100% sugarcane juice from variety RB867515 and 0.5% aconitate. Toget... Mostrar Tudo |
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Palavras-Chave: |
Internal normalizing; Organic acids; Relative expression; Sugar transport. |
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Thesaurus Nal: |
nitrogen fixation. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 14 | |
| 10. |  | RICCI, M. S. F.; ALMEIDA, F. M. F. D.; GUERRA, J. G. M.; GOCHETO JUNIOR, D. G.; RIBEIRO, R. L. D. Cultivo orgânico de cultivares de cebola nas condições da Baixada Fluminense. Horticultura Brasileira, Brasília, DF v. 32, n. 1, p. 120-124, jan./mar. 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | FREITAS-SILVA, O.; OLIVEIRA, T. Q.; CUNHA, F. Q.; SOUZA, M. L. M.; GODOY, R. L. O.; RICCI, M. S. F. Quality of shaded and unshaded organic coffee: presence of fungi, ochratoxin A and caffeine content. In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22., 2008, Campinas. Programme. Abstracts. Lausanne: Association for Science and Information on Coffee, 2008. p. 122, PC752.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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| 13. | | AGUIAR-MENEZES, E. de L.; SOUZA, S. A. S.; SANTOS, C. M. A.; RESENDE, A. L. S.; CAMPOS, J. M.; LIXA, A. T.; COSTA, J. R.; RICCI, M. S. F. Ocorrência de parasitóides de moscas-das-frutas (Diptera: tephritoidea) em seis cultivares de café Arábica sob manejo orgânico arborizado e a pleno sol. Seropédica: Embrapa Agrobiologia, 2007. 4 p. Parceria: UFRRJ. Trabalho apresentado no SICONBIOL, Brasília/DF, 30/06 a 04/07/2007.| Tipo: Folder/Folheto/Cartilha |
| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | AGUIAR-MENEZES, E. de L.; SOUZA, S. A. S.; SANTOS, C. M. A. dos; RESENDE, A. L. dos S.; CAMPOS, J. M.; LIXA, A. T.; COSTA, J. R.; RICCI, M. S. F. Ocorrência de parasitóides de moscas-das-frutas (Diptera: Tephritoidea) em seis cultivares de café arábica sob manejo orgânico arborizado e a pleno sol. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 10., 2007, Brasília, DF. Inovar para preservar a vida. Resumos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. 1 p. 1 CD-ROM. SICONBIOL. Parceria: UFRRJ| Tipo: Resumo em Anais de Congresso | Circulação/Nível: -- - -- |
| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 14 | |
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