| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
21/09/2017 |
|
Data da última atualização: |
21/09/2017 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
BEARSON, B. L.; BEARSON, S. M. D.; BRUNELLE, B. W.; BAYLES, D. O.; LEE, I. S.; KICH, J. D. |
|
Afiliação: |
BRADLEY L. BEARSON, USDA/ARS/National Laboratory for Agriculture and the Environment; SHAWN M. D. BEARSON, USDA/ARS/National Animal Disease Center; BRIAN W. BRUNELLE, USDA/ARS/National Animal Disease Center; DARRELL O. BAYLES, USDA/ARS/National Animal Disease Center; IN SOO LEE, Hannam University; JALUSA DEON KICH, CNPSA. |
|
Título: |
Salmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine. |
|
Ano de publicação: |
2017 |
|
Fonte/Imprenta: |
Journal of Medical Microbiology, v. 66, p. 651-661, 2017. |
|
DOI: |
10.1099/jmm.0.000482 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits. MenosAbstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, whic... Mostrar Tudo |
|
Thesagro: |
Salmonella Typhimurium; Suinocultura; Vacina; Virologia. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02678naa a2200241 a 4500
001 2076069
005 2017-09-21
008 2017 bl uuuu u00u1 u #d
024 7 $a10.1099/jmm.0.000482$2DOI
100 1 $aBEARSON, B. L.
245 $aSalmonella DIVA vaccine reduces disease, colonization and shedding due to virulent S. Typhimurium infection in swine.$h[electronic resource]
260 $c2017
520 $aAbstract Purpose. Non-host-adapted Salmonella serovars, including the common human food-borne pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), are opportunistic pathogens that can colonize food-producing animals without causing overt disease. Interventions against Salmonella are needed to enhance food safety, protect animal health and allow the differentiation of infected from vaccinated animals (DIVA). Methodology. An attenuated S. Typhimurium DIVA vaccine (BBS 866) was characterized for the protection of pigs following challenge with virulent S. Typhimurium. The porcine transcriptional response to BBS 866 vaccination was evaluated. RNASeq analysis was used to compare gene expression between BBS 866 and its parent; phenotypic assays were performed to confirm transcriptional differences observed between the strains. Results. Vaccination significantly reduced fever and interferon-gamma (IFNg) levels in swine challenged with virulent S. Typhimurium compared to mock-vaccinated pigs. Salmonella faecal shedding and gastrointestinal tissue colonization were significantly lower in vaccinated swine. RNA-Seq analysis comparing BBS 866 to its parental S. Typhimurium strain demonstrated reduced expression of the genes involved in cellular invasion and bacterial motility; decreased invasion of porcinederived IPEC-J2 cells and swimming motility for the vaccine strain was consistent with the RNA-Seq analysis. Numerous membrane proteins were differentially expressed, which was an anticipated gene expression pattern due to the targeted deletion of several regulatory genes in the vaccine strain. RNA-Seq analysis indicated that genes involved in the porcine immune and inflammatory response were differentially regulated at 2 days post-vaccination compared to pre-vaccination. Conclusion. Evaluation of the S. Typhimurium DIVA vaccine indicates that vaccination will provide both swine health and food safety benefits.
650 $aSalmonella Typhimurium
650 $aSuinocultura
650 $aVacina
650 $aVirologia
700 1 $aBEARSON, S. M. D.
700 1 $aBRUNELLE, B. W.
700 1 $aBAYLES, D. O.
700 1 $aLEE, I. S.
700 1 $aKICH, J. D.
773 $tJournal of Medical Microbiology$gv. 66, p. 651-661, 2017.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 11 | |
| 1. |  | GUERRERO, F. C.; GEISSLER, L. O.; MELO, M.; MEDEIROS, L. G. B.; MONTE, F. G.; NAVA, D. E. Consumo foliar de Spodoptera cosmioides (Walker, 1858) (Lepidoptera: Noctuidae) em mamona e pinhão-manso. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
|   |
| 2. | | MARANGON, R. B.; ZIMMER, V.; MONTE, F. G.; SILVA, S. D. dos A. e; NAVA, D. E. Infestação de Diatraea saccharalis (Fabr.,1794) (Lep.: crambidae) em cana-de-açúcar nas quatro últimas safras. In: CONGRESSO DE INICIAÇÃO CIENTÍFICA, 20.; MOSTRA CIENTÍFICA, 3., 2011, Pelotas. [Anais.]. Pelotas: UFPel, 2011. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
|  |
| 3. | | FILHO, R. C. B.; PRASTISSOLI, D.; NAVA, D. E.; MONTE, F. G.; GUIDONI, A. L.; POLANCZYK, R. A. Biologia de Pachycoris torridus (Hemiptera: Scutelleridae) em diferentes hospedeiros. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
|  |
| 4. | | BORGES FILHO, R. C.; MONTE, F. G.; NAVA, D. E.; PRATISSOLI, D.; POLANCZYK, R. A.; RAMOS, F. A.; LOIÁCONO, M. S. Parasitismo de ovos de Pachycoris torridus por Telenomus pachycoris na cultura do pinhão-manso no rio grande do sul. In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 12., 2011, São Paulo. Mudanças climáticas e sustentabilidade: quebra de paradigmas: anais. São Paulo: Instituto Biológico, 2011.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 5. | | MARANGON, R. B.; ZIMMER, V.; MONTE, F. G.; FILHO, R. C. B.; SILVA, S. D. dos A. e; NAVA, D. E. Desenvolvimento de Diatraea saccharalis (Lepidoptera: Crambidae) em dieta artificial com diferentes concentrações de sacarose. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 6. | | MARANGON, R. B.; ZIMMER, V.; MONTE, F. G.; RAUL FILHO, C. B.; SILVA, S. D. dos A. e; NAVA, D. E. Desenvolvimento de Diatraea saccharalis (Lepidoptera: Crambidae) em dieta artificial com diferentes concentrações de sacarose. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 24., 2012, Curitiba. SEB - 40 anos de avanços da Ciência Entomológica Brasileira. Curitiba: SEB, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 7. | | KNEIB, R. B.; VILLELA, J. C. B.; MOREIRA, L. L.; LEMÕES, J. S.; MONTE, F. G.; PINHEIRO, N. L.; SILVA, S. D. dos A. e. Caracterização molecular de acessos de milho crioulo atrvés de marcadores microssatélites associados ao caráter tolerância ao encharcamento. In: CONGRESSO DE INICIAÇÃO CIENTIFICA, 18.; ENCONTRO DE PÓS GRADUAÇÃO, 11., E MOSTRA CIENTIFICA, 1., 2009, Pelotas. Anais... Pelotas: UFPel, 2009. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 8. | | BORGES FILHO, R. da C.; PRATISSOLI, D. P.; NAVA, D. E.; MONTE, F. G.; GUIDONI, A. L.; SILVA, S. D. dos A. e; POLANCZYK, R. A. Development of Pachycoris torridus (Hemiptera: Scutelleridae) on Jatropha curcas (Euphorbiaceae), Psidium cattleianum (Myrtaceae) and Aleurites fordii (Euphorbiaceae). Florida Entomologist, v. 96, n. 3, p. 1149-1157, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 9. | | MONTE, F. G.; BORGES FILHO, R. da C. B.; HUBNER, L. K.; NAVA, D. E.; MELO, M.; MARANGON, R. B.; SILVA, S. D. dos A. e. Infestação de Diatraea saccharalis em cana-de-açúcar nas safras agrícolas de 2007/08, 2008/09 e 2009/10. In: SIMPÓSIO ESTADUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA ANUAL DE AGROENERGIA, 3.; REUNIÃO TÉCNICA DA MANDIOCA, 10.; REUNIÃO TÉCNICA DA BATATA-DOCE, 2., Pelotas, 2010. Anais... Pelotas: Embrapa Clima Temperado, 2010. Organizado por Sérgio Delmar dos Anjos e Silva, Ivan Rodrigues de Almeida e Ana Cláudia Barneche de Oliveira. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 10. | | BORGES FILHO, R. C.; NAVA, D. E.; PRATISSOLI, D.; GUIDONI, A. L.; MONTE, F. G.; POLONCZYK, R. A.; LOIÁCONO, M. S. Biologia em diferentes temperaturas e exigências térmicas de Telenomus pachycoris (Hymenoptera: Scelionidae) em ovos de Pachycoris torridus (Hemiptera: Scutelleridae). In: SIMPÓSIO DE CONTROLE BIOLÓGICO, 13., 2013, Bonito, MS. Anais... Brasília, DF: Embrapa, 2013. 1 CD-ROM. SICONBIOL.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 11. | | ZIMMES, V.; BORGES FILHO, R da C.; MONTE, F. G.; GUIDONI, A. L.; SILVA, S. D. dos A. e; NAVA, D. E. Tabela de vida de fertilidade de pachycoris torridus (hemiptera: scutelleridae) em pinhão-manso, araçá e tungue. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA E PÓS-GRADUAÇÃO DA EMBRAPA CLIMA TEMPERADO, 4., 2012, Pelotas. Ciência e inovação para 2050: qual o futuro que queremos? Resumos e palestras... Pelotas: Embrapa Clima Temperado, 2012. 1 CD-ROM.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| Registros recuperados : 11 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
