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 | Acesso ao texto completo restrito à biblioteca da Embrapa Semiárido. Para informações adicionais entre em contato com cpatsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
08/06/2020 |
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Data da última atualização: |
12/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPESTRINI, L. H.; RASERA, G. B.; CAMARGO, A. C. de; FRANCHIN, M.; NANI, B. D.; ROSALEN, P. L.; CANNIATTI-BRAZACA, S. G.; BIASOTO, A. C. T.; SHAHIDIC, F.; ALENCAR, S. M. |
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Afiliação: |
Luciano Henrique Campestrini; Gabriela Boscariol Rasera; Adriano Costa de Camargo; Marcelo Franchin; Bruno Dias Nani; Pedro Luiz Rosalen; Solange Guidolin Canniatti-Brazaca; ALINE TELLES BIASOTO MARQUES, CPATSA; Fereidoon Shahidic; Severino Matias Alencar. |
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Título: |
Alkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Food Research International, v. 131, may 2020. |
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DOI: |
https://doi.org/10.1016/j.foodres.2019.108532 |
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Idioma: |
Inglês |
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Conteúdo: |
Winemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains. MenosWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory c... Mostrar Tudo |
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Palavras-Chave: |
Atividade anti-inflamatória; Extração sequencial; Polissacarídeos; Vinificação. |
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Thesagro: |
Pectina; Subproduto; Uva. |
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Thesaurus Nal: |
Grapes; Polysaccharides. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02892naa a2200349 a 4500
001 2123132
005 2020-06-12
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.foodres.2019.108532$2DOI
100 1 $aCAMPESTRINI, L. H.
245 $aAlkaline conditions better extract anti-inflammatory polysaccharides from winemaking by-products.$h[electronic resource]
260 $c2020
520 $aWinemaking generates large amounts of by-products, a well recognized source of phenolic compounds. However, less attention has been paid to the polysaccharide-rich fraction (PRF) and effects of fractionation techniques on its potential bioactivity. Therefore, PRFs from Syrah and Tempranillo winemaking by-products were extracted under aqueous (neutral pH conditions), acidic and alkaline conditions. PRFs were screened for their monosaccharide composition, uronic acid content, homogeneity and molecular weight. Anti-inflammatory activity of PRFs were evaluated on stimulated RAW 264.7 macrophages. PRF obtained in water and/or under acidic conditions showed heterogeneous profiles. As like as in the others, a heterogeneous and complex profile was detected in extracts procured under alkaline conditions. A high content of uronic acid was found in aqueous extracts, thus indicating the presence of pectin. Pectin and hemicellulose were present in PRFs procured under acidic conditions. Alkaline conditions rendered extracts containing a complex mixture of monosaccharides, mainly xylose. This latter PRF was the only one exhibiting anti-inflammatory potential (at 100 ?g/mL) by reducing the release of TNF-? and activation of NF-?B in LPS-activated RAW 264.7 macrophages, with no effect on cell viability. Regardless of the grape variety, PRFs obtained under alkaline conditions were the best option to obtain bioactive polysaccharides with potential application as a source of anti-inflammatory compounds. A complex mixture of polymers may be responsible for the anti-inflammatory effects. Finally, according to results procured by NMR, it is possible to suggest that bioactive fractions are composed of a chain of ?-L-Araf-(1 ? 3) linked, ?-D-Xylp- (1 ? 4), ?-D-Glcp-(1 ? 4) linked, ?-D-GalpA-(1 ? 4), ?-D-Gal-(1 ? 2) forming possible RG I and RG II and xylan chains.
650 $aGrapes
650 $aPolysaccharides
650 $aPectina
650 $aSubproduto
650 $aUva
653 $aAtividade anti-inflamatória
653 $aExtração sequencial
653 $aPolissacarídeos
653 $aVinificação
700 1 $aRASERA, G. B.
700 1 $aCAMARGO, A. C. de
700 1 $aFRANCHIN, M.
700 1 $aNANI, B. D.
700 1 $aROSALEN, P. L.
700 1 $aCANNIATTI-BRAZACA, S. G.
700 1 $aBIASOTO, A. C. T.
700 1 $aSHAHIDIC, F.
700 1 $aALENCAR, S. M.
773 $tFood Research International$gv. 131, may 2020.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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| Registros recuperados : 16 | |
| 1. |  | SOARES, A. B.; SARTOR, L. R.; ADAMI, P. F.; VARELLA, A. C.; FONSECA, L.; MEZZALIRA, J. C. Influência da luminosidade no comportamento de onze espécies forrageiras perenes de verão. Revista Brasileira de Zootecnia, Viçosa, v. 38, n. 3, p. 443-451, 2009.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 2. | | CARVALHO, P. C. de F.; TRINDADE, J. K. da; MEZZALIRA, J. C.; POLI, C. H. E. C.; NABINGER, C.; GENRO, T. C. M.; GONDA, H. L. Do bocado ao pastoreio de precisão: compreendendo a interface planta-animal para explorar a multi-funcionalidade das pastagens. Revista Brasileira de Zootecnia, Viçosa, v. 38, p. 109-122, jul. 2009. Suplemento especial.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 3. | | BREMM, C.; CARVALHO, P. C. F.; PEREZ, N. B.; FONSECA, L.; AMARAL, G. A.; MEZZALIRA, J. C.; SOUZA, G. C.; DAVID, D. B. de. Prehension patterns of beef heifers in a complex vegetation mosaic. In: INTERNATIONAL RANGELAND CONGRESS, 9., 2011, Rosario, Argentina. Diverse rangelands for a sustainable society: [proceedings...]. Rosario: Instituto Nacional de Tecnología Agropecuaria; Asociación Argentina para el Manejo de Pastizales Naturales, 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
|  |
| 4. | | MEZZALIRA, J. C.; CARVALHO, P. C. F.; AMARAL, M. F.; BREMM, C.; TRINDADE, J. K. da; GONÇALVES, E. N.; MARTINS, R. S.; GENRO, T. C. M. Patterns of sward height reduction in a tropical pasture grazed by dairy cows. In: GENERAL MEETING OF THE EUROPEAN GRASSLAND FEDERATION, 23., 2010, Kiel. Grassland in a changing world. Duderstadt: Mecke-Druck, 2010. p. 973-975. EGF 2010. Metas 2011.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
|  |
| 5. | | SOARES, A. B.; CARVALHO, P. C. de F.; NABINGER, C.; TRINDADE, J. P. P.; DA TRINDADE, J. K.; MEZZALIRA, J. C. Dinâmica da composição botânica numa pastagem natural sob efeito de diferentes ofertas de forragem. Ciência Rural, Santa Maria, v. 41, n. 8, p. 1459-1465, ago. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 6. | | BREMM, C.; CARVALHO, P. C. F.; FONSECA, L.; AMARAL, G. A.; MEZZALIRA, J. C.; PEREZ, N. B.; NABINGER, C.; LACA, E. A. Diet switching by mammalian herbivores in response to exotic grass invasion. Plos One, v. 11, n. 2, e0150167, 26 fev. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 7. | | MEZZALIRA, J. C.; CARVALHO, P. C. F.; AMARAL, M. F.; BREMM, C.; TRINDADE, J. K.; GONÇALVES, E. N.; GENRO, T. C. M.; SUÑÉ, R. W. Manejo do milheto em pastoreio rotativo para maximizar a taxa de ingestão por vacas leiteiras. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 65, n. 3, p. 833-840, jun. 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 8. | | MEZZALIRA, J. C.; FONSECA, L.; BONNET, O.; MEZZALIRA, C. C.; ROZA, L. da F.; PAGANI, L. C.; GENRO, T. C. M.; CARVALHO, P. C. de F. Relationship between grazing jaw movements and time per bite in cattle: effect of sward structure and grazing methods. In: INTERNATIONAL GRASSLAND CONGRESS, 22., 2013, Sidney. Revitalising grasslands to sustain our communities: proceedings. New South Wales: MCI Australia, 2013. p. 1188-1189. Editors David L. Michalk, Geoffrey D. Millar, Warwick B. Badgery, Kim M. Broadfoot.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 9. | | AMARAL, M. F.; MEZZALIRA, J. C.; BREMM, C.; TRINDADE, J. K.; GIBB, M. J.; SUÑÉ, R. W.; CARVALHO, P. C. de F. Sward structure management for a maximum short-term intake rate in annual ryegrass. Grass and Forage Science, Oxford, v. 68, n. 2, p. 271-277, June 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 10. | | TRINDADE, J. K. da; PINTO, C. E.; NEVES, F. P.; MEZZALIRA, J. C.; BREMM, C.; GENRO, T. C. M.; TISCHLER, M. R.; NABINGER, C.; GONDA, H. L.; CARVALHO, P. C. F. Forage allowance as a target of grazing management: implications on grazing time and forage searching. Rangeland Ecology & Management, Wheat Ridge, v. 65, n. 4, p. 382-393, July 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 11. | | CARVALHO, P. C. F.; BREMM, C.; MEZZALIRA, J. C.; FONSECA, L.; TRINDADE, J. K.; BONNET, O. J. F.; TISCHLER, M.; GENRO, T. C. M.; NABINGER, C.; LACA, E. A. Can animal performance be predicted from short-term grazing processes? Animal Production Science, v. 55, n. 3, p. 319-327, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 12. | | TRINDADE, J. K. da; NEVES, F. P.; PINTO, C. E.; BREMM, C.; MEZZALIRA, J. C.; NADIN, L. B.; GENRO, T. C. M.; GONDA, H. L.; CARVALHO, P. C. F. Daily forage intake by cattle on natural grassland: response to forage allowance and sward structure. Rangeland Ecology & Management, v. 69, n. 1, p. 59-67, Jan. 2016.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 13. | | MEZZALIRA, J. C.; FONSECA, L.; MEZZALIRA, C. C.; ROZA, L. da F.; PAGANI, L. C.; EBBING, M. A.; GENRO, T. C. M.; CARVALHO, P. C. de F.; BREMM, C.; ARRUDA FILHO, R. S.; CORREIA, L. H. S.; CANGIANO, C. Handling time and bite mass mechanisms in large herbivores: contrasts between sward structure and grazing methods. In: INTERNATIONAL GRASSLAND CONGRESS, 22., 2013, Sidney. Revitalising grasslands to sustain our communities: proceedings. New South Wales: MCI Australia, 2013. p. 1169-1170. Editors David L. Michalk, Geoffrey D. Millar, Warwick B. Badgery, Kim M. Broadfoot.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| 14. | | SAVIAN, J. C.; SCHONS, R. M. T.; SCHNEIDER, E. A. N.; PENSO, J. F.; CAETANO, L. A. M.; MARCHI, D. E.; MEZZALIRA, J. C.; BERNDT, A.; BAYER, C.; Carvalho, P. C. F. A new sward management strategy to mitigate the methane (CH4) emissions by grazing ruminants. In: GREENHOUSE GAS AND ANIMAL AGRICULTURE CONFERENCE, 6., Melbourne, Australia. Proceedings... Melbourne, Australia: GGAA, 2016. p. 79.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | SAVIAN, J. V.; SCHONS, R. M. T.; MARCHI, D. E.; FREITAS, T. S. de; SILVA NETO, G. F. da; MEZZALIRA, J. C.; BERNDT, A.; BAYER, C.; CARVALHO, P. C. de F. Rotatinuous stocking: a grazing management innovation that has high potential to mitigate methane emissions by sheep. Journal of Cleaner Production, v.186, p.602-608, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | CARVALHO, P. C. de F.; TRINDADE, J. K. da; SILVA, S. C. da; BREMM, C.; MEZZALIRA, J. C.; NABINGER, C.; AMARAL, M. F.; CARASSAI, I. J.; MARTINS, R. S.; GENRO, T. C. M.; GONÇALVES, E. N.; AMARAL, G. A. do; GONDA, H. L.; POLI, C. H. E. C.; SANTOS, D. T. dos. Consumo de forragem por animais em pastejo: analogias e simulações em pastoreio rotativo. In: SIMPÓSIO SOBRE MANEJO DA PASTAGEM, 25., 2009, Piracicaba. Intensificação de sistemas de produção animal em pasto: anais. Piracicaba: FEALQ, 2009. p. 61-93. Editores Sila Carneiro da Silva, Carlos Guilherme Silveira Pedreira, José Carlos de Moura, Vidal Pedroso de Faria.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Pecuária Sul. |
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| Registros recuperados : 16 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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