| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
30/08/2018 |
|
Data da última atualização: |
30/08/2018 |
|
Autoria: |
SANTOS, A. C.; LEITE, F. P. L.; VIANNA, A. M.; WEEGE, G. B.; FINGER, I. S.; MULLER, V.; CURCIO, B. R.; NOGUEIRA, C. E. W. |
|
Afiliação: |
Alice C. Santos, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Fábio P. L. Leite, Centro de Desenvolvimento Tecnológico - UFPel; Ana M. Vianna, Centro de Desenvolvimento Tecnológico - UFPel; Guilherme B. Weege, Laboratório de Parasitologia Molecular e Imunologia - UFPel; Ilusca S. Finger, Centro de Desenvolvimento Tecnológico - UFPel; Vitória Müller, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Bruna R. Curcio, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel; Carlos E. W. Nogueira, Faculdade de Veterinária/Universidade Federal de Pelotas - UFPel. |
|
Título: |
Dynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 6, p. 1105-1109, junho 2018 |
|
Idioma: |
Inglês |
|
Notas: |
Título em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi. |
|
Conteúdo: |
Theileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking. MenosTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares prese... Mostrar Tudo |
|
Palavras-Chave: |
Hemoprotozoan; Hemoprotozoário; Imunidade humoral; Piroplasmose equina; REMA-2. |
|
Thesagro: |
Eqüino. |
|
Thesaurus Nal: |
Equine piroplasmosis; Horses; Humoral immunity; Parasitoses; Theileria equi. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182208/1/Dynamics-of-humoral-immune-response-in-pregnant-mares.pdf
|
|
Marc: |
LEADER 03294naa a2200349 a 4500
001 2094866
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. C.
245 $aDynamics of humoral immune response in pregnant mares and foals vaccinated with Theileria equi recombinant EMA-2.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Dinâmica da resposta imune humoral em éguas gestantes e potros vacinados com EMA-2 recombinante de Theileria equi.
520 $aTheileria equi is an infectious hemoprotozoan agent of equine piroplasmosis, a disease that has severe economic and sanitary impact internationally. In addition to its common clinical features, piroplasmosis can cause gestational losses and neonatal damage, which makes neonates susceptible to this disease. The aim of this study was to evaluate the dynamics of humoral immune response to recombinant EMA-2 of T. equi in pregnant mares and foals, as well as the transfer of vaccine antibodies through the colostrum ingested by sucking foals. For vaccine production, the EMA-2 expression gene was cloned and expressed in the yeast species, Pichia pastoris. Thirty-six horses were used, of which 18 were pregnant mares and 18 were foals. The mares were divided into control and vaccinated groups, and the vaccinated group received three doses of rEMA-2 every 21 days starting at 300 days of gestation. Foals from vaccinated and control groups were evaluated until the sixth month of life. The production of antibodies by foals on the rEMA-2 vaccination schedule was also evaluated from the second month of life. Foals in the vaccinated group had received three doses of the vaccine every 21 days. The method used to evaluate serum and colostrum samples was indirect ELISA, and plates were sensitized with the rEMA-2 protein. At the end of the vaccination schedule, vaccinated mares showed a 2.3-fold increase in antibody levels when compared to baseline values. The colostrum of vaccinated mares presented antibody levels of 1.0432±0.33. Foals delivered by vaccinated mares presented levels of antibodies greater than those of foals delivered by control mares after their first time sucking (at about twelve hours after birth). Foals vaccinated in the second month of life showed an 8.3-fold increase in antibody levels when compared to baseline values. The vaccination schedule with rEMA-2 was able to stimulate humoral immunity in pregnant mares. Vaccine immunoglobins were concentrated in the colostrum of vaccinated mares and foals delivered by these mares showed an increase in serum levels of vaccine antibodies after the first-time sucking.
650 $aEquine piroplasmosis
650 $aHorses
650 $aHumoral immunity
650 $aParasitoses
650 $aTheileria equi
650 $aEqüino
653 $aHemoprotozoan
653 $aHemoprotozoário
653 $aImunidade humoral
653 $aPiroplasmose equina
653 $aREMA-2
700 1 $aLEITE, F. P. L.
700 1 $aVIANNA, A. M.
700 1 $aWEEGE, G. B.
700 1 $aFINGER, I. S.
700 1 $aMULLER, V.
700 1 $aCURCIO, B. R.
700 1 $aNOGUEIRA, C. E. W.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 6, p. 1105-1109, junho 2018
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 45 | |
| 7. |  | NICOLAO, G.; MAYER, N. A.; UENO, B.; BIANCHI, V. J. Production, productivity, and yield efficiency of ‘BRS-Kampai’ own-rooted peach trees and budded trees on clonal rootstocks. Applied Fruit Science, v. 67, n. 2, p. 2948-2623, 2025.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Clima Temperado. |
|    |
| 8. | | SANTANA, F. M.; BIANCHI, V. J.; RODRIGUES, L. N.; RADKE, A. K.; CLEBSCH, C. C. Análise da diversidade genética de isolados de Pyrenophora tritici-repentis por meio de marcadores microssatélites. Tropical Plant Pathology, v. 34, p. S133, ago. 2009. Suplemento, ref 484. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Brasília, DF, ago. 2009.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
| |
| 12. | | ROSA, G. G. da; ZANANDREA, I.; MAYER, N. A.; BIANCHI, V. J. Propagação de porta-enxerto de Prunus spp. por estaquia: efeito do genótipo, do estádio de desenvolvimento do ramo e tipo de estaca¹. Revista Ceres, Viçosa, v. 64, n.1, p. 090-097, 2017.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
|  |
| 13. | | MAYER, N. A.; BIANCHI, V. J.; CASTRO, L. A. S. de. Propagação e produção de mudas. In: MAYER, N. A.; FRANZON, R. C.; RASEIRA, M. do C. B. (Ed.). Pêssego, nectarina e ameixa: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2019. 290 p. il. (Coleção 500 perguntas, 500 respostas). cap. 6. 79-106| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 16. | | CASAGRANDE JUNIOR, J. G.; BIANCHI, V. J.; STRELOW, E. Z.; BACARIN, M. A.; FACHINELLO, J. C. Influência do sombreamento sobre os teores de carboidratos e fenóis em estacas semilenhosas de araçazeiro. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 34, n. 12, p. 2219-2223, nov. 1999 Título em inglês: Influence of shading on the carbohydrates and phenols in cattley guava cutting.| Biblioteca(s): Embrapa Unidades Centrais. |
| |
| 18. | | RADMANN, E. B.; BIANCHI, V. J.; SOUZA, T. M.; FACHINELLO, J. C.; OLIVEIRA, R. P. de. Influência da composição do meio de cultivo e do tipo de explante na micropropagação do porta-enxerto de Prunus sp. "GXN-9". Scientia Agraria, Curitiba, v. 10, n. 2, p. 095-101, 2009. p. 095-101| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| 19. | | ROSA, G. G. da; DUTRA, L. F.; MAYER, N. A.; BIANCHI, V. J. Influencia de la composición del medio de cultivo en la propagación in vitro de portainjerto de Prunus spp. 'marianna 2624'.influence of culture medium composition on Prunus spp. rootstock 'marianna 2624' in vitro propagation. In: REUNIÓN ARGENTINA DE FISIOLOGÍA VEGETAL, 31., 2016, Corrientes. Enfrentando el cambio climático haciendo sostenible Ia productividad agro-forestal. Corrientes, Argentina, SAFV, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Clima Temperado. |
| |
| Registros recuperados : 45 | |
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
