|
|
Registro Completo |
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
18/03/2008 |
Data da última atualização: |
19/03/2008 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Autoria: |
FERREIRA, M. E.; MORETZSOHN, M. de C.; BUSO, G. S. C. |
Afiliação: |
Marcio Elias Ferreira, Embrapa Recursos Genéticos e Biotecnologia; Marcio de Carvalho Moretzsohn, Embrapa Recursos Genéticos e Biotecnologia; Gláucia Salles Cortopassi Buso, Embrapa Recursos Genéticos e Biotecnologia. |
Título: |
Fundamentos de caracterização molecular de germoplasma vegetal. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: NASS, L. L. (Ed.). Recursos genéticos vegetais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007. |
Páginas: |
p. 377-420. |
ISBN: |
978-85-87697-34-9 |
Idioma: |
Português |
Palavras-Chave: |
CAPS; Caracterização molecular; Coleção nuclear; Conservação ex situ; cpDNA; Diversidade genética; Estrutura genética; Genoma cloroplástico; Genoma mitocondrial; Genoma nuclear; Germoplasma vegetal; Identidade genética; Marcadores moleculares; Minissatélites; mtDNA; PCR; RAPD; RFLP; Riqueza alélica; Similaridade genética; SSR. |
Thesagro: |
Ácido Nucléico; Polimorfismo. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01241naa a2200433 a 4500 001 1189369 005 2008-03-19 008 2007 bl uuuu u00u1 u #d 020 $a978-85-87697-34-9 100 1 $aFERREIRA, M. E. 245 $aFundamentos de caracterização molecular de germoplasma vegetal. 260 $c2007 300 $ap. 377-420. 650 $aÁcido Nucléico 650 $aPolimorfismo 653 $aCAPS 653 $aCaracterização molecular 653 $aColeção nuclear 653 $aConservação ex situ 653 $acpDNA 653 $aDiversidade genética 653 $aEstrutura genética 653 $aGenoma cloroplástico 653 $aGenoma mitocondrial 653 $aGenoma nuclear 653 $aGermoplasma vegetal 653 $aIdentidade genética 653 $aMarcadores moleculares 653 $aMinissatélites 653 $amtDNA 653 $aPCR 653 $aRAPD 653 $aRFLP 653 $aRiqueza alélica 653 $aSimilaridade genética 653 $aSSR 700 1 $aMORETZSOHN, M. de C. 700 1 $aBUSO, G. S. C. 773 $tIn: NASS, L. L. (Ed.). Recursos genéticos vegetais. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2007.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
18/05/2007 |
Data da última atualização: |
08/06/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
ZUCCHI, T. D.; ZUCCHI, F. D.; POLI, P.; MELO, I. S. de; ZUCCHI, T. M. A. D. |
Afiliação: |
Tiago Domingues Zucchi, ICB-USP; Fernando Domingues Zucchi, ICB-USP; Paola Poli, Dipartimento di Genetica Antropologia Evoluzione, Universita di Parma; ITAMAR SOARES DE MELO, CNPMA; Tania M. A. D. Zucchi, ICB-USP. |
Título: |
A short-term test adapted to detect the genotoxic effects of environmental volatile pollutants (benzene fumes) using the filamentous fungus Aspergillus nidulans. |
Ano de publicação: |
2005 |
Fonte/Imprenta: |
Journal of Environmental Monitoring, Washington, DC, v. 7, n. 6, p. 598-602, 2005. |
Idioma: |
Inglês |
Conteúdo: |
With the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the genotoxic/carcinogenic harmful side effects widely connected to benzene. This is the first description of a sensitive, rapid and inexpensive test able to detect the submutagenic dose effects of volatile environmental compounds. In addition, despite concentrating on benzene the same test can be applied to many other pollutants, volatile or not. Additionally, the test can also be used to detect the antigenotoxic properties of foods and drugs. MenosWith the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the geno... Mostrar Tudo |
Palavras-Chave: |
Microbiologia ambiental. |
Thesagro: |
Aspergillus Nidulans; Fungo; Microbiologia; Poluente; Poluição ambiental. |
Thesaurus NAL: |
Carcinogens. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 02770naa a2200253 a 4500 001 1015437 005 2015-06-08 008 2005 bl uuuu u00u1 u #d 100 1 $aZUCCHI, T. D. 245 $aA short-term test adapted to detect the genotoxic effects of environmental volatile pollutants (benzene fumes) using the filamentous fungus Aspergillus nidulans.$h[electronic resource] 260 $c2005 520 $aWith the recent focus on environmental problems, increasing awareness of the harmful effects of industrial and agricultural pollution has created a demand for progressively more sophisticated pollutant and toxicity detection methods. Using Aspergillus nidulans strains this work presents a new short term-test that, most importantly, enables the rapid and inexpensive detection of volatile pollutants that induce genotoxic/carcinogenic effects in animals. The main aim is to contribute to environmental health protection, and special attention is directed to monitoring the hazard posed by benzene (as a carcinogenic agent model) mainly because its ubiquitous presence often leads to severe noxious effects in humans among whom increased rates of human leukemia have been reported. To evaluate even the submutagenic effects of benzene fumes, two Aspergillus nidulans diploid strains, heterozygous for several auxotrophic mutations, were used. The DNA lesions produced stimulate mitotic recombination and homozygotization of auxotrophic recessive mutations. Conidial exposure to a saturated atmosphere of benzene fumes for 20 s was enough to increase the mitotic recombination frequencies significantly. Genetic analyses of treated diploids evidenced alterations related to mitotic recombination frequencies, gene expression, and allelic segregation rates. Altogether they reflect the potential of benzene to induce alterations in the fungal DNA, and albeit indirectly, they also respond for the genotoxic/carcinogenic harmful side effects widely connected to benzene. This is the first description of a sensitive, rapid and inexpensive test able to detect the submutagenic dose effects of volatile environmental compounds. In addition, despite concentrating on benzene the same test can be applied to many other pollutants, volatile or not. Additionally, the test can also be used to detect the antigenotoxic properties of foods and drugs. 650 $aCarcinogens 650 $aAspergillus Nidulans 650 $aFungo 650 $aMicrobiologia 650 $aPoluente 650 $aPoluição ambiental 653 $aMicrobiologia ambiental 700 1 $aZUCCHI, F. D. 700 1 $aPOLI, P. 700 1 $aMELO, I. S. de 700 1 $aZUCCHI, T. M. A. D. 773 $tJournal of Environmental Monitoring, Washington, DC$gv. 7, n. 6, p. 598-602, 2005.
Download
Esconder MarcMostrar Marc Completo |
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
Expressão de busca inválida. Verifique!!! |
|
|