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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
14/09/2012 |
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Data da última atualização: |
19/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MENEZES, S. P.; SANTOS, J. L. dos; CARDOSO, T. H. S.; PIROVANI, C. P.; MICHELI, F.; NORONHA, F. S. M.; ALVES, A. C.; FARIA, A. M. C.; GESTEIRA, A. da S. |
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Afiliação: |
SARA PEREIRA MENEZES, UESC; JANE LIMA DOS SANTOS, UESC; THYAGO HERMYLLY SANTANA CARDOSO, UESC; CARLOS PRIMINHO PIROVANI, UESC; FABIENNE MICHELI, UESC; FÁTIMA SOARES MOTTA NORONHA, UFMG; ANDRÉA CATÃO ALVES, UFMG; ANA MARIA CAETANO FARIA, UFMG; ABELMON DA SILVA GESTEIRA, CNPMF. |
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Título: |
Evaluation of the allergenicity potential of TcPR-10 protein from theobroma protein from theobroma cacao. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
PLos One, v. 7, Issue 6, June 2012. |
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ISSN: |
1932-6203 |
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Idioma: |
Português |
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Conteúdo: |
Background: The pathogenesis related protein PR10 (TcPR-10), obtained from the Theobroma cacao-Moniliophthora perniciosa interaction library, presents antifungal activity against M. perniciosa and acts in vitro as a ribonuclease. However, despite its biotechnological potential, the TcPR-10 has the P-loop motif similar to those of some allergenic proteins such as Bet v 1 (Betula verrucosa) and Pru av 1 (Prunus avium). The insertion of mutations in this motif can produce proteins with reduced allergenic power. The objective of the present work was to evaluate the allergenic potential of the wild type and mutant recombinant TcPR-10 using bioinformatics tools and immunological assays. Methodology/Principal Findings: Mutant substitutions (T10P, I30V, H45S) were inserted in the TcPR-10 gene by sitedirected mutagenesis, cloned into pET28a and expressed in Escherichia coli BL21(DE3) cells. Changes in molecular surface caused by the mutant substitutions was evaluated by comparative protein modeling using the three-dimensional structure of the major cherry allergen, Pru av 1 as a template. The immunological assays were carried out in 8?12 week old female BALB/c mice. The mice were sensitized with the proteins (wild type and mutants) via subcutaneous and challenged intranasal for induction of allergic airway inflammation.,Conclusions/Significance: We showed that the wild TcPR-10 protein has allergenic potential, whereas the insertion of mutations produced proteins with reduced capacity of IgE production and cellular infiltration in the lungs. On the other hand, in vitro assays show that the TcPR-10 mutants still present antifungal and ribonuclease activity against M. perniciosa RNA. In conclusion, the mutant proteins. MenosBackground: The pathogenesis related protein PR10 (TcPR-10), obtained from the Theobroma cacao-Moniliophthora perniciosa interaction library, presents antifungal activity against M. perniciosa and acts in vitro as a ribonuclease. However, despite its biotechnological potential, the TcPR-10 has the P-loop motif similar to those of some allergenic proteins such as Bet v 1 (Betula verrucosa) and Pru av 1 (Prunus avium). The insertion of mutations in this motif can produce proteins with reduced allergenic power. The objective of the present work was to evaluate the allergenic potential of the wild type and mutant recombinant TcPR-10 using bioinformatics tools and immunological assays. Methodology/Principal Findings: Mutant substitutions (T10P, I30V, H45S) were inserted in the TcPR-10 gene by sitedirected mutagenesis, cloned into pET28a and expressed in Escherichia coli BL21(DE3) cells. Changes in molecular surface caused by the mutant substitutions was evaluated by comparative protein modeling using the three-dimensional structure of the major cherry allergen, Pru av 1 as a template. The immunological assays were carried out in 8?12 week old female BALB/c mice. The mice were sensitized with the proteins (wild type and mutants) via subcutaneous and challenged intranasal for induction of allergic airway inflammation.,Conclusions/Significance: We showed that the wild TcPR-10 protein has allergenic potential, whereas the insertion of mutations produced proteins with reduced capacity... Mostrar Tudo |
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Palavras-Chave: |
Allergenic potentia; Protein PR10. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02473naa a2200253 a 4500
001 1933790
005 2023-06-19
008 2012 bl uuuu u00u1 u #d
022 $a1932-6203
100 1 $aMENEZES, S. P.
245 $aEvaluation of the allergenicity potential of TcPR-10 protein from theobroma protein from theobroma cacao.$h[electronic resource]
260 $c2012
520 $aBackground: The pathogenesis related protein PR10 (TcPR-10), obtained from the Theobroma cacao-Moniliophthora perniciosa interaction library, presents antifungal activity against M. perniciosa and acts in vitro as a ribonuclease. However, despite its biotechnological potential, the TcPR-10 has the P-loop motif similar to those of some allergenic proteins such as Bet v 1 (Betula verrucosa) and Pru av 1 (Prunus avium). The insertion of mutations in this motif can produce proteins with reduced allergenic power. The objective of the present work was to evaluate the allergenic potential of the wild type and mutant recombinant TcPR-10 using bioinformatics tools and immunological assays. Methodology/Principal Findings: Mutant substitutions (T10P, I30V, H45S) were inserted in the TcPR-10 gene by sitedirected mutagenesis, cloned into pET28a and expressed in Escherichia coli BL21(DE3) cells. Changes in molecular surface caused by the mutant substitutions was evaluated by comparative protein modeling using the three-dimensional structure of the major cherry allergen, Pru av 1 as a template. The immunological assays were carried out in 8?12 week old female BALB/c mice. The mice were sensitized with the proteins (wild type and mutants) via subcutaneous and challenged intranasal for induction of allergic airway inflammation.,Conclusions/Significance: We showed that the wild TcPR-10 protein has allergenic potential, whereas the insertion of mutations produced proteins with reduced capacity of IgE production and cellular infiltration in the lungs. On the other hand, in vitro assays show that the TcPR-10 mutants still present antifungal and ribonuclease activity against M. perniciosa RNA. In conclusion, the mutant proteins.
653 $aAllergenic potentia
653 $aProtein PR10
700 1 $aSANTOS, J. L. dos
700 1 $aCARDOSO, T. H. S.
700 1 $aPIROVANI, C. P.
700 1 $aMICHELI, F.
700 1 $aNORONHA, F. S. M.
700 1 $aALVES, A. C.
700 1 $aFARIA, A. M. C.
700 1 $aGESTEIRA, A. da S.
773 $tPLos One$gv. 7, Issue 6, June 2012.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | MORIKAWA, V. M.; ZIMPEL, C. K.; PAPLOSKI, I. A. D.; KIKUTI, M.; CASTRO, V.; LOPES, M. O.; BIONDO, A. W.; BARROS FILHO, I. R. de. Serological survey of anti-Leptospira spp. antibodies in Barbary sheep (Ammotragus lervia) at the Curitiba Zoo, southern Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 143-146, janeiro 2018. Título em português: Estudo sorológico de anticorpos anti-Leptospira spp. em aoudads (Ammotragus lervia) no zoológico de Curitiba, sul do Brasil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | MANTOVANI, C.; SUNIGA, P. A. P.; SANTOS, M. G. dos; EGITO, A. A. do; VERBISCK, N. V.; SANTOS, L. R. dos; ZIMPEL, C. K.; GUIMARÃES, A. M. S.; NASSAR, A. F. de C.; ARAUJO, F. R. The draft genome sequence of Burkholderia mallei BAC 86/19 isolated from a horse with glanders in Brazil. In: CONGRESO DE MICROBIOLOGÍA VETERINARIA, 2., Bueno Aires, 2023. Un pilar en el marco de una salud: libro de resúmenes. Tandil: Universidad Nacional del Centro de la Provincia de Buenos Aires, 2024. P. 114 BAC042.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | CARNEIRO, P. A.; ZIMPEL, C. K.; PASQUATTI, T. N.; SILVA-PEREIRA, T. T.; TAKATANI, H.; SILVA, C. B. D. G.; ABRAMOVITH, R. B.; GUIMARAES, A. M. S.; DAVILA, A. M. R.; ARAUJO, F. R.; KANEENE, J. B. Genetic Diversity and Potential Paths of Transmission of Mycobacterium bovis in the Amazon: the Discovery of M. bovis Lineage Lb1 Circulating in South America. Frontiers in Veterinary Science, v. 8, Article 630989, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | SUNIGA, P. A. P.; MANTOVANI, C.; SANTOS, M. G. dos; EGITO, A. A. do; VERBISCK, N. V.; SANTOS, L. R. dos; DÁVILA, A. M. R.; ZIMPEL, C. K.; ZERPA, M. C. S.; CHIEBAO, D. P.; GUIMARÃES, A. M. DE S.; NASSAR, A. F. DE C.; ARAUJO, F. R. Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing. Pathogens, v. 12, e1250, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Gado de Corte. |
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