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Registros recuperados : 92 | |
6. | | CALEGARIO, R. F.; FERREIRA, S. de S.; ANDRADE, E. C. de; ZERBINI, F. M. Characterization of Tomato yellow spot virus, a novel tomato-infecting begomovirus in Brazil Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 9, p. 1335-1343, set. 2007 Título em português: Caracterização do Tomato yellow spot virus, um novo begomovírus isolado de tomateiro no Brasil. Biblioteca(s): Embrapa Hortaliças; Embrapa Unidades Centrais. |
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9. | | CALEGARIO, R. F.; ANDRADE, E. C.; FERREIRA, S. S.; MANHANI, G. G.; ZERBINI, F. M. Biological and molecular characterization of a tomato isolate of Sidra micrantha mosaic vírus (SimMV). Fitopatologia Brasileira, Brasília, DF, v. 30, p. S180, ago. 2006. Suplemento. Resumo 749. Trabalho apresentado no 38. Congresso Brasileiro de Fitopatologia, 2005, Brasília, DF. Biblioteca(s): Embrapa Hortaliças. |
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10. | | CALEGARIO, R. F.; ALFENAS, P. F.; COTRIM, M. A.; ANDRADE, E. C.; ZERBINI, F. M. Caracterization of a new tomato-infecting begomovirus and its potencial use a vector for transient gene expression in plants. Virus Reviews & Research, Florianópolis, v. 8, p. 193-194, 2003. Resumo. Trabalho apresentado no 14º National Meeting of Virology, Florianópolis, 2003. Biblioteca(s): Embrapa Hortaliças. |
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11. | | COTRIM, M. A. de A.; KRAUSE-SAKATE, R.; NARITA, N.; ZERBINI, F. M.; PAVAN, M. A. Diversidade genética de begomovírus em cultivos de tomateiro no Centro-Oeste Paulista. Symma Phytopathologica, Botucatu, v. 3, n. 3, p. 300-303, jul./set. 2007. Biblioteca(s): Embrapa Agricultura Digital; Embrapa Hortaliças. |
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13. | | PEREIRA, Á. J.; ALFENAS-ZERBINI, P.; CASCARDO, R. S.; ANDRADE, E. C. de; ZERBINI, F. M. Analysis of the full-length genome sequence of papaya lethal yellowing virus (PLYV), determined by deep sequencing, confirms its classification in the genus Sobemovirus. Archives of Virology, v. 157, p. 2009-2011, 2012. Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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17. | | CARDOSO, M. S.; MARCELINO, F. C.; ZERBINI, F. M.; BARROS, E. G. Construção de um vetor viral baseado no DNA-A do tomato rugose mosaic virus (ToRMV) para a indução de silenciamento gênico em soja. In: CONGRESSO BRASILEIRO DE SOJA, 5.; MERCOSOJA 2009, Goiânia. Resumos... Londrina: Embrapa Soja, 2009. p. 99, trab. 166. Editado por Adilson de Oliveira Júnior, Odilon Ferreira Saraiva, Clara Beatriz Hoffmann Campo, César de Castro. Biblioteca(s): Embrapa Soja. |
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18. | | BARROS, D. R.; LIMA, A. T. M.; FERNANDES, J. J.; CARVALHO, M. G.; ZERBINI, F. M. Expression in Escherichia coli of the ac5 orf from tomato rugose mosaic virus (TORMV), and production of an ac5-specific antiserum. Virus Reviews & Research, Florianópolis, v. 8, p. 193, 2003. Resumo. Trabalho apresentado no 14º National Meeting of Virology, Florianópolis, 2003. Biblioteca(s): Embrapa Hortaliças. |
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19. | | FAJARDO, T. V. M.; BARROS, D. R.; NICKEL, O.; KUHN, G. B.; ZERBINI, F. M. Expression of grapevine leafroll-associated virus 3 coat protein gene in Escherichia coli and production of polyclonal antibodies. Fitopatologia Brasileira, Brasília, v. 32, n. 6, p. 43-47, 2007. Biblioteca(s): Embrapa Uva e Vinho. |
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20. | | AMBROZEVÍCIUS, L. P.; ANDRADE, E. C.; CALEGARIO, R. F.; FONTES, E. P. B.; ZERBINI, F. M. Molecular cloning and characterization of TGV-Ig, a tomato-infecting Begomovirus from Igarapé, Minas Gerais State. Virus Reviews & Research, Florianópolis, v. 5, n. 2, p. 196, 2000. Suplemento 1. Resumo. Trabalho apresentado no 11º National Meeting of Virology,São Lourenço, 2000. Biblioteca(s): Embrapa Hortaliças. |
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Registros recuperados : 92 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
05/03/2013 |
Data da última atualização: |
25/03/2013 |
Autoria: |
CARVALHO, S. L. de; SILVA, F. N. da; ZANARDO, L. G.; ALMEIDA, A. M. R.; ZERBINI, F. M.; CARVALHO, C. M. |
Afiliação: |
SILVIA L. DE CARVALHO, UFV; FÁBIO N. DA SILVA, UFV; LARISSA G. ZANARDO, UFV; ALVARO MANUEL RODRIGUES ALMEIDA, CNPSO; F. MURILO ZERBINI, UFV; CLAUDINE M. CARVALHO, UFV. |
Título: |
Production of polyclonal antiserum against Cowpea mild mottle virus coat protein and its application in virus detection. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Tropical Plant Patology, Brasília, DF, v. 38, n. 1, p. 49-54, Jan.-Feb. 2013. |
ISSN: |
1982-5676 |
Idioma: |
Inglês |
Conteúdo: |
Cowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Brazilian CpMMV isolate was cloned into a bacterial expression vector and transformed into Escherichia coli BL21::DE3 for in vitro expression. The coat protein, fused to a His-tag, was purified under denaturing conditions by affinity chromatography using a Ni-NTA resin. After renaturation, the integrity and identity of the purified recombinant protein was confirmed by SDS-Page and MALDI-ToF/ToF mass spectrometer analyses. A rabbit was immunized with increasing amounts of the recombinant protein. The specificity and sensitivity of the antiserum was demonstrated by Western blot and indirect ELISA assays. The polyclonal antisera raised against recombinant coat protein proved to be a reliable tool for CpMMV detection. |
Thesagro: |
Doença de planta; Soja; Vírus. |
Thesaurus NAL: |
Cowpea mild mottle virus; Plant diseases and disorders; Soybeans. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/77969/1/ID-34307.pdf
|
Marc: |
LEADER 01905naa a2200265 a 4500 001 1952212 005 2013-03-25 008 2013 bl uuuu u00u1 u #d 022 $a1982-5676 100 1 $aCARVALHO, S. L. de 245 $aProduction of polyclonal antiserum against Cowpea mild mottle virus coat protein and its application in virus detection.$h[electronic resource] 260 $c2013 520 $aCowpea mild mottle virus (CpMMV), the causal agent of stem necrosis disease, has drawn attention of soybean producers in recent years due to yield losses in the main producing regions of Brazil. Serological methods for viral detection require the use of an antiserum of good quality to achieve specificity and sensitivity. The entire coat protein gene of a Brazilian CpMMV isolate was cloned into a bacterial expression vector and transformed into Escherichia coli BL21::DE3 for in vitro expression. The coat protein, fused to a His-tag, was purified under denaturing conditions by affinity chromatography using a Ni-NTA resin. After renaturation, the integrity and identity of the purified recombinant protein was confirmed by SDS-Page and MALDI-ToF/ToF mass spectrometer analyses. A rabbit was immunized with increasing amounts of the recombinant protein. The specificity and sensitivity of the antiserum was demonstrated by Western blot and indirect ELISA assays. The polyclonal antisera raised against recombinant coat protein proved to be a reliable tool for CpMMV detection. 650 $aCowpea mild mottle virus 650 $aPlant diseases and disorders 650 $aSoybeans 650 $aDoença de planta 650 $aSoja 650 $aVírus 700 1 $aSILVA, F. N. da 700 1 $aZANARDO, L. G. 700 1 $aALMEIDA, A. M. R. 700 1 $aZERBINI, F. M. 700 1 $aCARVALHO, C. M. 773 $tTropical Plant Patology, Brasília, DF$gv. 38, n. 1, p. 49-54, Jan.-Feb. 2013.
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Embrapa Soja (CNPSO) |
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