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| Registros recuperados : 81 | |
| 41. |  | WOYANN, L. G.; ZDZIARSKI, A. D.; ZANELLA, R.; ROSA, A. C.; CASTRO, R. L. de; CAIERÃO, E.; TOIGO, M. D.; STORCK, L.; WU, J.; BENIN, G. Genetic gain over 30 years of spring wheat breeding in Brazil. Crop Science, v. 59, Sep./Oct. 2019.| Biblioteca(s): Embrapa Trigo. |
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| 42. | | ZANELLA, R.; GAVA, D.; PEIXOTO, J. de O.; SCHAEFER, R.; ZANELLA, J. R. C.; BIONDO, N.; SILVA, M. V. G. B.; CANTAO, M. E.; LEDUR, M. C. Genetic markers associated with influenza vaccination efficacy in swine. In: INTERNATIONAL SYMPOSIUM ON EMERGING AND RE-EMERGING PIG DISEASES, 7., 2015, Kyoto. Proceedings... Kyoto: Science Council of Japan; JSVS; JPVS; JASV, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 43. | | CAMARGO, J. de; MARQUES, M. G.; FACCIN, J. C. V.; JORGE NETO, P. N.; KICH, J. D.; SCARIOT, C. A.; ZANELLA, R. The feasibility of a swine semen storage system produced with bacteriostatic molecules to control bacterial proliferation. In: INTERNATIONAL SYMPOSIUM ON ANIMAL BIOLOGY OF REPRODUCTION, 9., 2022, Bento Gonçalves. Abstracts...Belo Horizonte: CBRA, 2022. Animal Reproduction, v. 19, n. 4, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 44. | | SILVA, A. N. da; ALVES, L.; OSOWSKI, G. V.; SABEI, L.; FERRAZ, P. A.; PUGLIESI, G.; MARQUES, M. G.; ZANELLA, R.; ZANELLA, A. J. Housing Conditions and a Challenge with Lipopolysaccharide on the Day of Estrus Can Influence Gene Expression of the Corpus Luteum in Gilts. Genes, v. 13, n. 5, p. 1-13, 2022.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 45. | | LAGO, L. V.; SILVA, A. N. da; ZANELLA, E. L.; MARQUES, M. G.; PEIXOTO, J. de O.; SILVA, M. V. G. B.; LEDUR, M. C.; ZANELLA, R. Identification of genetic regions associated with scrotal hernias in a commercial swine herd. Veterinary Sciences, v. 5, n. 15, 2018.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Suínos e Aves. |
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| 46. | | JOAQUIM, L. B.; ZANELLA, R.; CANTAO, M. E.; PEIXOTO, J. de O.; LEDUR, M. C.; SAVEGNAGO, R. P.; MUNARI, D. P. Inbreeding estimates using three different approaches in a swine breeding population. In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 47. | | ROMERO TISOT, C. M.; ZANELLA, R.; SOUZA, A. P.; LÖF, L. M.; SOARES, J. C. M.; MARQUES, M. G.; ZANELLA, E. L. Melatonin effect on cryopreserved sperm cells of crioulo stallions. Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 48. | | FACIOLI, F. L.; DE MARCHI, F.; MARQUES, M. G.; MICHELON, P. R. P.; ZANELLA, E. L.; CAIRES, K. C.; REEVES, J. J.; ZANELLA, R. The outcome and economic viability of embryo production using IVF and SOV techniques in the wagyu breed of cattle. Veterinary Sciences, v. 7, n. 58, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 49. | | FACIOLI, F. L.; BEZUTTI, G. da F.; BENDER, R. S.; MARQUES, M. G.; BONDAN, C.; ZANELLA, E. L; BERTOLINI, M.; ZANELLA, R. A rare case of heteropaternal twin calves after natural mating in Brazil. Animal Reproduction, v. 17, n. 4, e20200217, 2020.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 50. | | ZANELLA, R.; PEIXOTO, J. de O.; IBELLI, A. M. G.; CANTAO, M. E.; SETTLES, M.; COUTINHO, L. L.; LEDUR, M. C. RNA-Seq to identify genes involved with femoral head necrosis in broilers. In: INTERNATIONAL PLANT & ANIMAL GENOME, 23., 2015, San Diego, CA. Abstracts? San Diego, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 51. | | SUPERTI, B. F. V.; SOUZA, A. P. de; MÜLLER, B. C.; SILVA, Z. da; ZANELLA, R.; ZANELLA, E. L.; MARQUES, M. G. Técnicas cirúrgicas para preparo de rufiões suínos. Suinocultura Industrial, Itu, ed. 281, ano 40, n. 02, p. 14-17, 2018.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 52. | | FORNARI, M. B.; ZANELLA, R.; IBELLI, A. M. G.; FERNANDES, L. T.; CANTAO, M. E.; SOCCOL, V. T.; LEDUR, M. C.; PEIXOTO, J. de O. Unraveling the associations of osteoprotegerin gene with production traits in a paternal broiler line. SpringerPlus, v. 3, n. 682, 2014.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 53. | | PETRY, B.; ZANELLA, R.; IBELLI, A. M. G.; FORNARI, M. B.; PANDOLFI, J. R. C.; CANTAO, M. E.; COUTINHO, L. L.; PEIXOTO, J. de O.; LEDUR, M. C. Unraveling the associations of SOST gene with production traits in an F2 Chicken Resource Population. In: CONGRESSO BRASILEIRO DE GENÉTICA, 59., 2013, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2013. p. 152.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 54. | | ZANELLA, R.; GAVA, D.; PEIXOTO, J. de O.; SCHAEFER, R.; CIACCI-ZANELLA, J. R.; BIONDO, N.; SILVA, M. V. G. B.; CANTÃO, M. E.; LEDUR, M. C. Unravelling the genetic components involved in the immune response of pigs vaccinated against influenza virus. Virus Research, v. 210, p. 327-336, 2015.| Biblioteca(s): Embrapa Gado de Leite. |
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| 55. | | ZANELLA, R.; GAVA, D.; PEIXOTO, J. de O.; SCHAEFER, R.; ZANELLA, J. R. C.; BIONDO, N.; SILVA, M. V. G. B.; CANTAO, M. E.; LEDUR, M. C. Unravelling the genetic components involved in the immune response of pigs vaccinated against influenza virus. Virus Research, v. 210, p. 327-336, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 56. | | ZANELLA, R.; IBELLI, A. M. G.; CANTAO, M. E.; PEIXOTO, J. de O.; PANDOLFI, J. R. C.; COUTINHO, L. L.; LEDUR, M. C. Unrevealing the genetic pathways involved with bone integrity traits in poultry. In: INTERNATIONAL SYMPOSIUM ON ANIMAL FUNCTIONAL GENOMICS, 5., 2013, Guarujá. Programme and abstract book... [S.l.: s.n.], 2013. p. 31. ISAFG 2013. AB. 25.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 57. | | KICH, J. D.; UTHE, J. J.; BENAVIDES, M. V.; CANTAO, M. E.; ZANELLA, R.; TUGGLE, C. K.; BEARSON, S. M. D. TLR4 Single Nucleotide Polymorphisms (SNPs) Associated with Salmonella Shedding in Pigs. In: SAFEPORK, 2013, Portland. Proceeding... Portland, 2013. 1 Pen drive.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 58. | | KICH, J. D.; UTHE, J. J.; BENAVIDES, M. V.; CANTAO, M. E.; ZANELLA, R.; TUGGLE, C. K.; BEARSON, S. M. D. TLR4 single nucleotide polymorphisms (SNPs) associated with Salmonella shedding in pigs. Journal of Applied Genetics, v. 55, n. 2, p. 267-71, 2014.| Biblioteca(s): Embrapa Pecuária Sul; Embrapa Suínos e Aves. |
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| 59. | | MARCHESI, J. A. P.; IBELLI, A. M. G.; CANTAO, M. E.; ZANELLA, R.; SETTLES, M. L.; PEIXOTO, J. de O.; COUTINHO, L. L.; LEDUR, M. C. Whole transcriptome analysis of pectoralis major muscle reveals differences in calcium signaling pathway between white striping affected and unaffected broilers. In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2015.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 60. | | TONATTO, M.; ZDZIARSKI, A. D.; MEIRA, D.; PANHO, M. C.; ZANELLA, R.; MENEGAZZI, C. P.; COLONELLI, L. L.; FERNANDES, R. A. T.; CAMPAGNOLLI, O. R.; BENIN, G. Yield potential of modern soybean cultivars under high and low input levels. Pesquisa Agropecuária Brasileira, v. 57, e02852, 2022. Título em português: Potencial de rendimento de cultivares de soja modernas sob níveis alto e baixo de insumos.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 81 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
10/08/2021 |
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Data da última atualização: |
10/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
C - 0 |
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Autoria: |
ROMERO TISOT, C. M.; ZANELLA, R.; SOUZA, A. P.; LÖF, L. M.; SOARES, J. C. M.; MARQUES, M. G.; ZANELLA, E. L. |
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Afiliação: |
CÂNDIDA MALDANER ROMERO TISOT, UPF; RICARDO ZANELLA, UPF; UDESC/Lages; LUCAS MALLMANN LÖF, UPF; UFSM; MARIANA GROKE MARQUES, CNPSA; ERALDO LOURENSO ZANELLA, UPF. |
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Título: |
Melatonin effect on cryopreserved sperm cells of crioulo stallions. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Open Access Journal of Veterinary Science & Research, v. 5, n. 2, 2020. |
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DOI: |
10.23880/oajvsr-16000202 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation. MenosAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addi... Mostrar Tudo |
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Palavras-Chave: |
Melatonina. |
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Thesagro: |
Criopreservação; Espermatozóide; Garanhão; Reprodução Animal. |
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Thesaurus NAL: |
Animal reproduction; Cryopreservation; Melatonin; Reactive oxygen species; Spermatozoa; Stallions. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/225036/1/final9560.pdf
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Marc: |
LEADER 02592naa a2200337 a 4500
001 2133434
005 2021-08-10
008 2020 bl uuuu u00u1 u #d
024 7 $a10.23880/oajvsr-16000202$2DOI
100 1 $aROMERO TISOT, C. M.
245 $aMelatonin effect on cryopreserved sperm cells of crioulo stallions.$h[electronic resource]
260 $c2020
520 $aAbstract: The freezing/thawing process of spermatozoa can cause cellular damage to the male gamete, decreasing the fertilization potential due to the increase in the production of reactive oxygen species (ROS). Melatonin is a potent endogenous antioxidant that protects the body against the damage caused by ROS. This study has evaluated different melatonin concentrations on the sperm viability of cryopreserved semen of Crioulo stallions. For that, three ejaculates were collected from five stallions diluted in a commercial extender followed by centrifugation and resuspension in a commercial freezing extender supplemented with 0; 1.25; 2.5. 5mM of Melatonin before the cryopreservation process. After thawing, the evaluation was performed assessing motility and flow cytometry evaluations: the plasma membrane integrity (PI), the integrity of the acrosomal membrane (FITC-PNA), mitochondrial membrane potential (JC1), and ROS generation (DCF-DA). Our results showed that sperm motility in the group without Melatonin and the 1.25mM group did not show the difference; however, the groups 2.5mM and 5mM presented a reduction in sperm motility. The 1.25 mM concentration was able to protect the plasma membrane during the cryopreservation process, in addition to showing a significant reduction in the production of ROS and increasing the percentage of sperm with integral acrosome. It can also be seen that high concentrations of Melatonin did not show beneficial effects. In conclusion, the addition of 1.25 mM of the Melatonin in Crioulo sperm cells showed to have a protective effect on the sperm cell during cryopreservation.
650 $aAnimal reproduction
650 $aCryopreservation
650 $aMelatonin
650 $aReactive oxygen species
650 $aSpermatozoa
650 $aStallions
650 $aCriopreservação
650 $aEspermatozóide
650 $aGaranhão
650 $aReprodução Animal
653 $aMelatonina
700 1 $aZANELLA, R.
700 1 $aSOUZA, A. P.
700 1 $aLÖF, L. M.
700 1 $aSOARES, J. C. M.
700 1 $aMARQUES, M. G.
700 1 $aZANELLA, E. L
773 $tOpen Access Journal of Veterinary Science & Research$gv. 5, n. 2, 2020.
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Embrapa Suínos e Aves (CNPSA) |
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