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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
27/09/2023 |
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Data da última atualização: |
27/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CÔRTES, M. V. de C. B.; BARBOSA, E. T.; OLIVEIRA, M. I. de S.; MACIEL, L. H. R.; LOBO JUNIOR, M.; CONTESINI, F. J.; FILIPPI, M. C. C. de; SILVA-LOBO, V. L. |
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Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ELDER TADEU BARBOSA, CNPAF; MAYTHSULENE INACIO DE SOUSA OLIVEIRA; LIRIEL HELEN RODRIGUES MACIEL, UNIVERSIDADE FEDERAL DE GOIÁS; MURILLO LOBO JUNIOR, CNPAF; FABIANO JARES CONTESINI, UNIVERSITY OF DENMARK; MARTA CRISTINA CORSI DE FILIPPI, CNPAF; VALACIA LEMES DA SILVA LOBO, CNPAF. |
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Título: |
Trichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system: A helpful tool for the study of biological control agents. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biological Control, v. 184, 105281, Sept. 2023. |
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ISSN: |
1049-9644 |
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DOI: |
https://doi.org/10.1016/j.biocontrol.2023.105281 |
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Idioma: |
Inglês |
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Conteúdo: |
This article reports the first successful marker-free genetic engineering of Trichoderma harzianum, a widely studied biocontrol agent with great potential for use in sustainable agriculture. T. harzianum has been shown to control plant diseases, promote plant growth, and improve plant tolerance to abiotic stress. A specific CRISPR/Cas9 gene-editing system, based in self-replicative AMA1-plasmid, was used to generate T. harzianum mutant strains, targeting the albA (pks4) and ku70 genes, involved in melanin synthesis and non-homologous end joining DNA repair pathway, respectively. The resulting albA-deficient strain displayed a loss of pigmentation in their conidia, confirming phenotypically and genotypically the efficacy of the CRISPR/Cas9 system in generating targeted gene knockouts in T. harzianum. A marker-free ?ku70 strain was also constructed, with the maintenance of its original fitness, providing a helpful tool for precise genetic engineering in T. harzianum, due to NHEJ-deficiency, able to be used in in vitro and in vivo (in planta) studies. The study highlights the potential of using CRISPR/Cas9 technology to facilitate the study of the molecular processes involved in the biocontrol and plant growth-promoting properties of T. harzianum. |
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Palavras-Chave: |
AMA1-plasmid; Biocontrol; Gene edition; Molecular technology; NHEJ. |
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Thesagro: |
Genética Molecular; Trichoderma Harzianum. |
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Thesaurus Nal: |
Abiotic stress; Genetic engineering; Plant growth-promoting rhizobacteria. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02401naa a2200349 a 4500
001 2156940
005 2023-09-27
008 2023 bl uuuu u00u1 u #d
022 $a1049-9644
024 7 $ahttps://doi.org/10.1016/j.biocontrol.2023.105281$2DOI
100 1 $aCÔRTES, M. V. de C. B.
245 $aTrichoderma harzianum marker-free strain construction based on efficient CRISPR/Cas9 recyclable system$bA helpful tool for the study of biological control agents.$h[electronic resource]
260 $c2023
520 $aThis article reports the first successful marker-free genetic engineering of Trichoderma harzianum, a widely studied biocontrol agent with great potential for use in sustainable agriculture. T. harzianum has been shown to control plant diseases, promote plant growth, and improve plant tolerance to abiotic stress. A specific CRISPR/Cas9 gene-editing system, based in self-replicative AMA1-plasmid, was used to generate T. harzianum mutant strains, targeting the albA (pks4) and ku70 genes, involved in melanin synthesis and non-homologous end joining DNA repair pathway, respectively. The resulting albA-deficient strain displayed a loss of pigmentation in their conidia, confirming phenotypically and genotypically the efficacy of the CRISPR/Cas9 system in generating targeted gene knockouts in T. harzianum. A marker-free ?ku70 strain was also constructed, with the maintenance of its original fitness, providing a helpful tool for precise genetic engineering in T. harzianum, due to NHEJ-deficiency, able to be used in in vitro and in vivo (in planta) studies. The study highlights the potential of using CRISPR/Cas9 technology to facilitate the study of the molecular processes involved in the biocontrol and plant growth-promoting properties of T. harzianum.
650 $aAbiotic stress
650 $aGenetic engineering
650 $aPlant growth-promoting rhizobacteria
650 $aGenética Molecular
650 $aTrichoderma Harzianum
653 $aAMA1-plasmid
653 $aBiocontrol
653 $aGene edition
653 $aMolecular technology
653 $aNHEJ
700 1 $aBARBOSA, E. T.
700 1 $aOLIVEIRA, M. I. de S.
700 1 $aMACIEL, L. H. R.
700 1 $aLOBO JUNIOR, M.
700 1 $aCONTESINI, F. J.
700 1 $aFILIPPI, M. C. C. de
700 1 $aSILVA-LOBO, V. L.
773 $tBiological Control$gv. 184, 105281, Sept. 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
04/11/2019 |
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Data da última atualização: |
04/11/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
BOIAGO, M. M.; DILKIN, J. D.; KOLM, M. A.; BARRETA, M.; SOUZA, C. F.; BALDISSERA, M. D.; SANTOS, I. D. dos; WAGNER, R.; TAVERNARI, F. de C.; SILVA, M. L. B. da; ZAMPAR, A.; STIVANIN, T. E.; SILVA, A. S. da. |
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Afiliação: |
MARCEL MANENTE BOIAGO, UDESC/Chapecó; JÉSSICA D. DILKIN, UDESC/Chapecó; MARINDIA A. KOLM, UDESC/Chapecó; MAURÍCIO BARRETA, UDESC/Chapecó; CARINE F. SOUZA, UFSM; MATHEUS D. BALDISSERA, UFSM; INGRID D. DOS SANTOS, UFSM; ROGER WAGNER, UFSM; FERNANDO DE CASTRO TAVERNARI, CNPSA; MARCIO LUIS BUSI DA SILVA; ALINE ZAMPAR, UDESC/Chapecó; TADIA E. STIVANIN; ALEKSANDRO SCHAEFER DA SILVA, UDESC/Chapecó. |
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Título: |
Spirulina platensis in Japanese quail feeding alters fatty acid profiles and improves egg quality: benefits to consumers. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Food Biochemistry, v. 43, n. 7, e12860, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The aim of this study was to investigate whether microalgae in Japanese quail feed al‐ters performance, fatty acid profiles in the eggs and egg quality. One hundred quails were distributed in four groups and five replicates of five birds per experimental group. The treatments consisted of four levels of Spirulina platensis microalgae (0%, 5%, 10%, and 15%) in the diets. We evaluated the productive performance and chemical‐physical characteristics of eggs, the oxidant/antioxidant status in egg yolks, and the fatty acid profile in the diet and egg yolks. Microalgae in the diet did not influence egg production; however, it increased the yolk index as well as the color intensity of the yolk. Saturated and polyunsaturated fatty acid levels decreased in egg yolks, and monounsaturated fatty acid levels increased in the yolks. Lipid peroxidation levels were lower and total antioxidant capacity was higher in egg yolks of quails receiving microalgae in the diet. Practical applications: Microalgae in quail diets improves egg quality and provides benefits to consumer health, acting as an antioxidant and immune‐stimulant. Microalgae in quail diets had positive effects on egg quality. This is because it reduced levels of saturated fatty acids that are undesirable, and increased monounsaturated fatty acid levels that are beneficial to the health of consumers. Finally, antioxidants increased in egg yolks, consequently reducing lipid peroxidation. |
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Palavras-Chave: |
Japanese quail; Qualidade do ovo. |
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Thesagro: |
Codorna; Dieta; Microalga. |
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Thesaurus NAL: |
Carotenoids; Coturnix japonica; Fatty acids; Food quality; Spirulina platensis; Yolk proteins. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02599naa a2200397 a 4500
001 2113844
005 2019-11-04
008 2019 bl uuuu u00u1 u #d
100 1 $aBOIAGO, M. M.
245 $aSpirulina platensis in Japanese quail feeding alters fatty acid profiles and improves egg quality$bbenefits to consumers.$h[electronic resource]
260 $c2019
520 $aAbstract: The aim of this study was to investigate whether microalgae in Japanese quail feed al‐ters performance, fatty acid profiles in the eggs and egg quality. One hundred quails were distributed in four groups and five replicates of five birds per experimental group. The treatments consisted of four levels of Spirulina platensis microalgae (0%, 5%, 10%, and 15%) in the diets. We evaluated the productive performance and chemical‐physical characteristics of eggs, the oxidant/antioxidant status in egg yolks, and the fatty acid profile in the diet and egg yolks. Microalgae in the diet did not influence egg production; however, it increased the yolk index as well as the color intensity of the yolk. Saturated and polyunsaturated fatty acid levels decreased in egg yolks, and monounsaturated fatty acid levels increased in the yolks. Lipid peroxidation levels were lower and total antioxidant capacity was higher in egg yolks of quails receiving microalgae in the diet. Practical applications: Microalgae in quail diets improves egg quality and provides benefits to consumer health, acting as an antioxidant and immune‐stimulant. Microalgae in quail diets had positive effects on egg quality. This is because it reduced levels of saturated fatty acids that are undesirable, and increased monounsaturated fatty acid levels that are beneficial to the health of consumers. Finally, antioxidants increased in egg yolks, consequently reducing lipid peroxidation.
650 $aCarotenoids
650 $aCoturnix japonica
650 $aFatty acids
650 $aFood quality
650 $aSpirulina platensis
650 $aYolk proteins
650 $aCodorna
650 $aDieta
650 $aMicroalga
653 $aJapanese quail
653 $aQualidade do ovo
700 1 $aDILKIN, J. D.
700 1 $aKOLM, M. A.
700 1 $aBARRETA, M.
700 1 $aSOUZA, C. F.
700 1 $aBALDISSERA, M. D.
700 1 $aSANTOS, I. D. dos
700 1 $aWAGNER, R.
700 1 $aTAVERNARI, F. de C.
700 1 $aSILVA, M. L. B. da
700 1 $aZAMPAR, A.
700 1 $aSTIVANIN, T. E.
700 1 $aSILVA, A. S. da
773 $tJournal of Food Biochemistry$gv. 43, n. 7, e12860, 2019.
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