| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Trigo. |
|
Data corrente: |
04/09/2014 |
|
Data da última atualização: |
01/02/2015 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ZHOU, G.; PEREIRA, J. F.; DELHAIZE, E.; ZHOU, M.; MAGALHAES, J. V.; RYAN, P. R. |
|
Afiliação: |
UNIVERSITY OF TASMANIA; JORGE FERNANDO PEREIRA, CNPT; CSIRO PLANT INDUSTRY; UNIVERSITY OF TASMANIA; JURANDIR VIEIRA DE MAGALHAES, CNPMS; CSIRO PLANT INDUSTRY. |
|
Título: |
Enhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3. |
|
Ano de publicação: |
2014 |
|
Fonte/Imprenta: |
Journal of Experimental Botany, London, v. 65, n. 9, p. 2381-2390, 2014. |
|
DOI: |
10.1093/jxb/eru121 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Malate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species. MenosMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tole... Mostrar Tudo |
|
Palavras-Chave: |
Citrato; Tolerância ao alumínio; Transgênico. |
|
Thesagro: |
Cevada; Solo Ácido. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/117026/1/2014-Journal-of-Experimental-Botany-v65n9p2381.pdf
|
|
Marc: |
LEADER 02283naa a2200253 a 4500
001 2007369
005 2015-02-01
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1093/jxb/eru121$2DOI
100 1 $aZHOU, G.
245 $aEnhancing the aluminium tolerance of barley by expressing the citrate transporter genes SbMATE and FRD3.$h[electronic resource]
260 $c2014
520 $aMalate and citrate efflux from root apices is a mechanism of Al3+ tolerance in many plant species. Citrate efflux is facilitated by members of the MATE (multidrug and toxic compound exudation) family localized to the plasma membrane of root cells. Barley (Hordeum vulgare) is among the most Al3+-sensitive cereal species but the small genotypic variation in tolerance that is present is correlated with citrate efflux via a MATE transporter named HvAACT1. This study used a biotechnological approach to increase the Al3+ tolerance of barley by transforming it with two MATE genes that encode citrate transporters: SbMATE is the major Al3+-tolerance gene from sorghum whereas FRD3 is involved with Fe nutrition in Arabidopsis. Independent transgenic and null T3 lines were generated for both transgenes. Lines expressing SbMATE showed Al3+-activated citrate efflux from root apices and greater tolerance to Al3+ toxicity than nulls in hydroponic and short-term soil trials. Transgenic lines expressing FRD3 exhibited similar phenotypes except citrate release from roots occurred constitutively. The Al3+ tolerance of these lines was compared with previously generated transgenic barley lines overexpressing the endogenous HvAACT1 gene and the TaALMT1 gene from wheat. Barley lines expressing TaALMT1 showed significantly greater Al3+ tolerance than all lines expressing MATE genes. This study highlights the relative efficacy of different organic anion transport proteins for increasing the Al3+ tolerance of an important crop species.
650 $aCevada
650 $aSolo Ácido
653 $aCitrato
653 $aTolerância ao alumínio
653 $aTransgênico
700 1 $aPEREIRA, J. F.
700 1 $aDELHAIZE, E.
700 1 $aZHOU, M.
700 1 $aMAGALHAES, J. V.
700 1 $aRYAN, P. R.
773 $tJournal of Experimental Botany, London$gv. 65, n. 9, p. 2381-2390, 2014.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Trigo (CNPT) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Semiárido. |
|
Data corrente: |
17/01/2011 |
|
Data da última atualização: |
17/04/2023 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
MARTINS, R. de J.; VESCHI, J. L. A.; CARMO, F. B.; AZEVEDO, V.; SEYFFERT, N; MIYOSHI, A.; MEYER, R.; PORTELA, R.; PEIXOTO, R. M.; COSTA, M. M. da; ZAFALON, L. F.; GOUVEIA, A. M. G. |
|
Afiliação: |
RAIANE DE JESUS MARTINS, CNPq; JOSIR LAINE APARECIDA VESCHI, CPATSA; FILIPE BORGES CARMO, UFMG; VASCO AZEVEDO, UFMG; NUBIA SEYFFERT, UFMG; ANDERSON MIYOSHI, UFMG; ROBERTO MEYER, UFBA; RICARDO PORTELA, UFBA; RODOLFO M. PEIXOTO, IF SERTÃO PERNAMBUCANO; MATEUS MATIUZI DA COSTA, UNIVASF; LUIZ FRANCISCO ZAFALON, CPPSE; AURORA M. GUIMARÃES GOUVEIA, UFMG. |
|
Título: |
Avaliação da presença de anticorpos anti-Corynebacterium pseudotuberculosis em caprinos leiteiros do Território do Sisal, BA. |
|
Ano de publicação: |
2010 |
|
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 5., 2010, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2010. |
|
Páginas: |
p. 25-31. |
|
Série: |
(Embrapa Semiárido. Documentos, 228.). |
|
Idioma: |
Português |
|
Conteúdo: |
O objetivo do presente trabalho foi avaliar a presença de anticorpos anti C. pseudotuberculosis em caprinos leiteiros do Território do Sisal, Bahia. |
|
Palavras-Chave: |
Anticorpos. |
|
Thesagro: |
Bactéria; Caprino; Corynebacterium Pseudotuberculosis; Doença; Epidemiologia; Linfadenite Caseosa. |
|
Thesaurus NAL: |
Epidemiology; Goats. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25582/1/Raiane.pdf
|
|
Marc: |
LEADER 01315nam a2200373 a 4500
001 1873277
005 2023-04-17
008 2010 bl uuuu u00u1 u #d
100 1 $aMARTINS, R. de J.
245 $aAvaliação da presença de anticorpos anti-Corynebacterium pseudotuberculosis em caprinos leiteiros do Território do Sisal, BA.$h[electronic resource]
260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 5., 2010, Petrolina. Anais... Petrolina: Embrapa Semiárido$c2010
300 $ap. 25-31.
490 $a(Embrapa Semiárido. Documentos, 228.).
520 $aO objetivo do presente trabalho foi avaliar a presença de anticorpos anti C. pseudotuberculosis em caprinos leiteiros do Território do Sisal, Bahia.
650 $aEpidemiology
650 $aGoats
650 $aBactéria
650 $aCaprino
650 $aCorynebacterium Pseudotuberculosis
650 $aDoença
650 $aEpidemiologia
650 $aLinfadenite Caseosa
653 $aAnticorpos
700 1 $aVESCHI, J. L. A.
700 1 $aCARMO, F. B.
700 1 $aAZEVEDO, V.
700 1 $aSEYFFERT, N
700 1 $aMIYOSHI, A.
700 1 $aMEYER, R.
700 1 $aPORTELA, R.
700 1 $aPEIXOTO, R. M.
700 1 $aCOSTA, M. M. da
700 1 $aZAFALON, L. F.
700 1 $aGOUVEIA, A. M. G.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
