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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/12/2001 |
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Data da última atualização: |
07/06/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOGUERCIO, L. L.; SANTOS, C. G.; BARRETO, M. R.; GUIMARAES, C. T.; PAIVA, E. |
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Afiliação: |
CLAUDIA TEIXEIRA GUIMARAES, CNPMS. |
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Título: |
Association of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, Oxford, v. 32, n. 5, p. 362-367, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To verify whether the presence of any of the cry1C. 1D, 1E and 1F genes could be associated with high toxicity against fall armyworm. Methods and Results: A sample of 60 strains from a large collections of tropical Bacillus thuringiensis (B.t.) isolates was subjected to feeding bioassay and gene-specific PCR. Positive amplification of cry-specific fragments, so confirmed by sequencing, revealed that cry1C was ubiquitous and distributed among high and low mortality classes, cry1D was underrepresented and showed no clear association to high toxicity, and cry1F was not detected. The presence of cry1E significantly correlated to high levels of insecticidal activity, as estimated by linear regression analysis. Conclusions: The PCR amplification of cry1E-specific fragments alone appears to be sufficient to identify B.t. strains with high mortality levels against tropical armyworm. Significance and Impact of the Study: The approach presented is promising as a simple and efficient method for first-tier, marker-assisted screening of environment-specific B.t. germplasm effective in controlling a single target pest. |
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Palavras-Chave: |
Armyworm; Lagarta do cartucho. |
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Thesagro: |
Controle Biológico; Spodoptera Frugiperda. |
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Thesaurus Nal: |
biological control. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01972naa a2200229 a 4500
001 1485179
005 2018-06-07
008 2001 bl uuuu u00u1 u #d
100 1 $aLOGUERCIO, L. L.
245 $aAssociation of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera$bNoctuidae) larvae.$h[electronic resource]
260 $c2001
520 $aAims: To verify whether the presence of any of the cry1C. 1D, 1E and 1F genes could be associated with high toxicity against fall armyworm. Methods and Results: A sample of 60 strains from a large collections of tropical Bacillus thuringiensis (B.t.) isolates was subjected to feeding bioassay and gene-specific PCR. Positive amplification of cry-specific fragments, so confirmed by sequencing, revealed that cry1C was ubiquitous and distributed among high and low mortality classes, cry1D was underrepresented and showed no clear association to high toxicity, and cry1F was not detected. The presence of cry1E significantly correlated to high levels of insecticidal activity, as estimated by linear regression analysis. Conclusions: The PCR amplification of cry1E-specific fragments alone appears to be sufficient to identify B.t. strains with high mortality levels against tropical armyworm. Significance and Impact of the Study: The approach presented is promising as a simple and efficient method for first-tier, marker-assisted screening of environment-specific B.t. germplasm effective in controlling a single target pest.
650 $abiological control
650 $aControle Biológico
650 $aSpodoptera Frugiperda
653 $aArmyworm
653 $aLagarta do cartucho
700 1 $aSANTOS, C. G.
700 1 $aBARRETO, M. R.
700 1 $aGUIMARAES, C. T.
700 1 $aPAIVA, E.
773 $tLetters in Applied Microbiology, Oxford$gv. 32, n. 5, p. 362-367, 2001.
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| 1. |  | MEYER, M. C.; YORINORI, J. T. SOUZA, N. L. A mela da soja - caracterização do patógeno, resistência genética e controle químico. In: REUNIÃO DE PESQUISA DE SOJA DA REGIÃO CENTRAL DO BRASIL, 24., 2002, São Pedro, SP. Resumos... Londrina: Embrapa Soja, 2002. p. 155156. (Embrapa Soja. Documentos, 185).| Biblioteca(s): Embrapa Soja. |
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