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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
20/09/2013 |
Data da última atualização: |
19/05/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
GARITA, L. C.; TASSI, A. D.; CALEGARIO, R. F.; KITAJIMA, E. W.; CARBONELL, S. A. M.; ASTUA, J. de F. |
Afiliação: |
L. C. GARITA, ESALQ; A. D. TASSI, ESALQ; R. F. CALEGARIO, ESALQ; E. W. KITAJIMA, ESALQ; S. A. M. CARBONELL, IAC; JULIANA DE FREITAS ASTUA, CNPMF. |
Título: |
Common bean: experimental indicator plant for Citrus leprosis virus C and some other Cytoplasmic-type brevipalpus-transmitted viruses. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Plant Disease, v. 97, n. 10, p. 1346, 2013. |
Idioma: |
Inglês |
Conteúdo: |
Citrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus vector host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean IAC Una was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with necrotic local lesions and may serve as test plants for these viruses. Two nuclear types of BrTV (Coffee ringspot virus and Clerodendrum chlorotic spot virus) were unable to produce symptoms on common bean. MenosCitrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus vector host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean IAC Una was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with n... Mostrar Tudo |
Thesagro: |
Brevipalpus Phoenicis; Phaseolus Vulgaris. |
Thesaurus Nal: |
Beans; Citrus leprosis virus C. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02426naa a2200229 a 4500 001 1966750 005 2023-05-19 008 2013 bl uuuu u00u1 u #d 100 1 $aGARITA, L. C. 245 $aCommon bean$bexperimental indicator plant for Citrus leprosis virus C and some other Cytoplasmic-type brevipalpus-transmitted viruses.$h[electronic resource] 260 $c2013 520 $aCitrus leprosis (CL) caused by Citrus leprosis virus C (CiLV-C) is present in Latin America from Mexico to Argentina, where citrus plants are grown. CiLV-C is transmitted by the tenuipalpid mite, Brevipalpus phoenicis, causing localized lesions on citrus leaves, fruit, and stems. One limitation to study of the virus vector host relationship in this pathosystem is the lack of a suitable assay plant. On Citrus spp. used as susceptible hosts, symptoms may take weeks or months to appear after experimental inoculation by viruliferous mites. Common bean (Phaseolus vulgaris) was found to respond with localized necrotic lesions after inoculation with viruliferous B. phoenicis in 5 days. Thus far, 113 tested common bean varieties and lines and some recent accessions of varied genetic background behaved in a similar way. Black bean IAC Una was adopted as a standard test variety. When inoculated leaves were left at 28 to 30°C, the period for the lesion appearance was reduced to only 2 days. Confirmation that the lesions on common bean leaves are caused by CiLV-C were made by transmission electron microscopy, immunofluorescence, enzyme-linked immunosorbent assay, and reverse-transcription polymerase chain reaction specific for CiLV-C. Common bean plants mite-inoculated with some other cytoplasmic-type Brevipalpus-transmitted viruses (BrTVs) (Passion fruit green spot virus, Solanum violaefolium ringspot virus, Ligustrum ringspot virus, and Hibiscus green spot virus) also responded with necrotic local lesions and may serve as test plants for these viruses. Two nuclear types of BrTV (Coffee ringspot virus and Clerodendrum chlorotic spot virus) were unable to produce symptoms on common bean. 650 $aBeans 650 $aCitrus leprosis virus C 650 $aBrevipalpus Phoenicis 650 $aPhaseolus Vulgaris 700 1 $aTASSI, A. D. 700 1 $aCALEGARIO, R. F. 700 1 $aKITAJIMA, E. W. 700 1 $aCARBONELL, S. A. M. 700 1 $aASTUA, J. de F. 773 $tPlant Disease$gv. 97, n. 10, p. 1346, 2013.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
Data corrente: |
17/12/2013 |
Data da última atualização: |
15/06/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 2 |
Autoria: |
McCLURE, M. C.; SONSTEGARD, T. S.; WIGGANS, G. R.; EENENNAAM, A. L. V.; WEBER, K. L.; PENEDO, C. T.; BERRY, D. P.; FLYNN, J.; GARCIA, J. F.; CARMO, A. S.; REGITANO, L. C. de A.; ALBUQUERQUE, M.; SILVA, M. V. G. B.; MACHADO, M. A.; COFFEY, M.; MOORE, K.; BOSCHER, M. Y.; GENESTOUT, L.; MAZZA, R.; TAYLOR, J. F.; SCHNABEL, R. D.; SIMPSON, B.; MARQUES, E.; McEWAN, J. C.; CROMIE, A.; COUTINHO, L. L.; KUEHN, L. A.; KEELE, J. W.; PIPER, E. K.; COOK, J.; WILLIAMS, R.; TASSELL, C. P. V. |
Afiliação: |
MATTHEW C. McCLURE, USDA; TAD S. SONSTEGARD, USDA; GEORGE R. WIGGANS, USDA; ALISON L. VAN EENENNAAM, University of California - Davis; KRISTINA L. WEBER, University of California-Davis; CECILIA T. PENEDO, University of California-Davis; DONAGH P. BERRY, Animal and Grassland Research and Innovation Centre, Ireland; JOHN FLYNN, Weatherbys DNA Laboratory, Ireland; JOSÉ F. GARCIA, UNESP; ADRIANA S. CARMO, Deoxi Biotecnologia, Araçatuba; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; MILLA ALBUQUERQUE, USP; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARCO ANTONIO MACHADO, CNPGL; MIKE COFFEY, SRUC, Scotland; KIRSTY MOORE, SRUC, Scotland; MARIE-YVONNE BOSCHER, LABOGENA, France; LUCIE GENESTOUT, LABOGENA, France; RAFFAELE MAZZA, Italian Breeders Association, Roma; JEREMY F. TAYLOR, University of Missouri-Columbia; ROBERT D. SCHNABEL, University of Missouri-Columbia; BARRY SIMPSON, GeneSeek, Neogen Company, Lincoln, USA; ELISA MARQUES, GeneSeek, Neogen Company, Lincoln, USA; JOHN C. McEWAN, AgResearch, Invermay Agricultural Centre, New Zealand; ANDREW CROMIE, Irish Cattle Breeding Federation, Ireland; LUIZ L. COUTINHO, ESALQ/USP; LARRY A. KUEHN, USDA-ARS; JOHN W. KEELE, USDA-ARS; EMILY K. PIPER, University of Queensland, Australia; JIM COOK, University of New England, Australia; ROBERT WILLIAMS, American-International Charolais Association, USA; CURTIS P. VAN TASSELL, USDA. |
Título: |
Imputation of microsatellite alleles from dense SNP genotypes for parentage verification across multiple Bos taurus and Bos indicus breeds. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Frontiers in Genetics, v. 4, n. 176, 2013. |
Páginas: |
11 p. |
Idioma: |
Inglês |
Conteúdo: |
To assist cattle producers transition from microsatellite (MS) to single nucleotide polymorphism (SNP) genotyping for parental verification we previously devised an effective and inexpensive method to impute MS alleles from SNP haplotypes. While the reported method was verified with only a limited data set (N = 479) from Brown Swiss, Guernsey, Holstein, and Jersey cattle, some of the MS-SNP haplotype associations were concordant across these phylogenetically diverse breeds. This implied that some haplotypes predate modern breed formation and remain in strong linkage disequilibrium. To expand the utility of MS allele imputation across breeds, MS and SNP data from more than 8000 animals representing 39 breeds (Bos taurus and B. indicus) were used to predict 9410 SNP haplotypes, incorporating an average of 73 SNPs per haplotype, for which alleles from 12 MS markers could be accurately be imputed. Approximately 25% of the MS-SNP haplotypes were present in multiple breeds (N = 2 to 36 breeds). These shared haplotypes allowed for MS imputation in breeds that were not represented in the reference population with only a small increase in Mendelian inheritance inconsistancies. Our reported reference haplotypes can be used for any cattle breed and the reported methods can be applied to any species to aid the transition from MS to SNP genetic markers. While ~91% of the animals with imputed alleles for 12 MS markers had ≤1 Mendelian inheritance conflicts with their parents' reported MS genotypes, this figure was 96% for our reference animals, indicating potential errors in the reported MS genotypes. The workflow we suggest autocorrects for genotyping errors and rare haplotypes, by MS genotyping animals whose imputed MS alleles fail parentage verification, and then incorporating those animals into the reference dataset. MenosTo assist cattle producers transition from microsatellite (MS) to single nucleotide polymorphism (SNP) genotyping for parental verification we previously devised an effective and inexpensive method to impute MS alleles from SNP haplotypes. While the reported method was verified with only a limited data set (N = 479) from Brown Swiss, Guernsey, Holstein, and Jersey cattle, some of the MS-SNP haplotype associations were concordant across these phylogenetically diverse breeds. This implied that some haplotypes predate modern breed formation and remain in strong linkage disequilibrium. To expand the utility of MS allele imputation across breeds, MS and SNP data from more than 8000 animals representing 39 breeds (Bos taurus and B. indicus) were used to predict 9410 SNP haplotypes, incorporating an average of 73 SNPs per haplotype, for which alleles from 12 MS markers could be accurately be imputed. Approximately 25% of the MS-SNP haplotypes were present in multiple breeds (N = 2 to 36 breeds). These shared haplotypes allowed for MS imputation in breeds that were not represented in the reference population with only a small increase in Mendelian inheritance inconsistancies. Our reported reference haplotypes can be used for any cattle breed and the reported methods can be applied to any species to aid the transition from MS to SNP genetic markers. While ~91% of the animals with imputed alleles for 12 MS markers had ≤1 Mendelian inheritance conflicts with their parents' reported MS ... Mostrar Tudo |
Palavras-Chave: |
Imputation; Microsatellite; Parentage verification; SNP; STR. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 03360naa a2200565 a 4500 001 2154448 005 2023-06-15 008 2013 bl uuuu u00u1 u #d 100 1 $aMcCLURE, M. C. 245 $aImputation of microsatellite alleles from dense SNP genotypes for parentage verification across multiple Bos taurus and Bos indicus breeds.$h[electronic resource] 260 $c2013 300 $a11 p. 520 $aTo assist cattle producers transition from microsatellite (MS) to single nucleotide polymorphism (SNP) genotyping for parental verification we previously devised an effective and inexpensive method to impute MS alleles from SNP haplotypes. While the reported method was verified with only a limited data set (N = 479) from Brown Swiss, Guernsey, Holstein, and Jersey cattle, some of the MS-SNP haplotype associations were concordant across these phylogenetically diverse breeds. This implied that some haplotypes predate modern breed formation and remain in strong linkage disequilibrium. To expand the utility of MS allele imputation across breeds, MS and SNP data from more than 8000 animals representing 39 breeds (Bos taurus and B. indicus) were used to predict 9410 SNP haplotypes, incorporating an average of 73 SNPs per haplotype, for which alleles from 12 MS markers could be accurately be imputed. Approximately 25% of the MS-SNP haplotypes were present in multiple breeds (N = 2 to 36 breeds). These shared haplotypes allowed for MS imputation in breeds that were not represented in the reference population with only a small increase in Mendelian inheritance inconsistancies. Our reported reference haplotypes can be used for any cattle breed and the reported methods can be applied to any species to aid the transition from MS to SNP genetic markers. While ~91% of the animals with imputed alleles for 12 MS markers had ≤1 Mendelian inheritance conflicts with their parents' reported MS genotypes, this figure was 96% for our reference animals, indicating potential errors in the reported MS genotypes. The workflow we suggest autocorrects for genotyping errors and rare haplotypes, by MS genotyping animals whose imputed MS alleles fail parentage verification, and then incorporating those animals into the reference dataset. 653 $aImputation 653 $aMicrosatellite 653 $aParentage verification 653 $aSNP 653 $aSTR 700 1 $aSONSTEGARD, T. S. 700 1 $aWIGGANS, G. R. 700 1 $aEENENNAAM, A. L. V. 700 1 $aWEBER, K. L. 700 1 $aPENEDO, C. T. 700 1 $aBERRY, D. P. 700 1 $aFLYNN, J. 700 1 $aGARCIA, J. F. 700 1 $aCARMO, A. S. 700 1 $aREGITANO, L. C. de A. 700 1 $aALBUQUERQUE, M. 700 1 $aSILVA, M. V. G. B. 700 1 $aMACHADO, M. A. 700 1 $aCOFFEY, M. 700 1 $aMOORE, K. 700 1 $aBOSCHER, M. Y. 700 1 $aGENESTOUT, L. 700 1 $aMAZZA, R. 700 1 $aTAYLOR, J. F. 700 1 $aSCHNABEL, R. D. 700 1 $aSIMPSON, B. 700 1 $aMARQUES, E. 700 1 $aMcEWAN, J. C. 700 1 $aCROMIE, A. 700 1 $aCOUTINHO, L. L. 700 1 $aKUEHN, L. A. 700 1 $aKEELE, J. W. 700 1 $aPIPER, E. K. 700 1 $aCOOK, J. 700 1 $aWILLIAMS, R. 700 1 $aTASSELL, C. P. V. 773 $tFrontiers in Genetics$gv. 4, n. 176, 2013.
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