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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/09/2023 |
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Data da última atualização: |
11/09/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
AYMÉE, L.; DI AZEVEDO, M. I. N.; REIS, L.; MENDES, J.; CASTRO, F. de D. A. de; CARVALHO-COSTA, F. A. C.; SOUZA, G. N. de; LILENBAUM, W. |
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Afiliação: |
LUIZA AYMÉE, UNIVERSIDADE FEDERAL FLUMINENSE; MARIA ISABEL NOGUEIRA DI AZEVEDO, UNIVERSIDADE FEDERAL FLUMINENSE; LUIZA REIS, UNIVERSIDADE FEDERAL FLUMINENSE; JULIA MENDES, UNIVERSIDADE FEDERAL FLUMINENSE; FÚLVIA DE FÁTIMA ALMEIDA DE CASTRO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; FILIPE ANIBAL CARVALHO-COSTA, INSTITUTO OSWALDO CRUZ; GUILHERME NUNES DE SOUZA, CNPGL; WALTER LILENBAUM, UNIVERSIDADE FEDERAL FLUMINENSE. |
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Título: |
Unconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Animals, v. 13, 2832, 2023. |
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DOI: |
https://doi.org/10.3390/ani13182832 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses. MenosBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular ana... Mostrar Tudo |
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Palavras-Chave: |
Abomasal fluid; Leptospiral infection; LipL32; Molecular analysis. |
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Thesagro: |
Aborto; Bovino; Gado; Leptospirose. |
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Thesaurus Nal: |
Abortion (animals); Cattle; Necropsy. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1156556/1/Unconventional-sites-for-diagnosis-of-Leptospirosis-in-bovine-anicteric-fetuses.pdf
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Marc: |
LEADER 03302naa a2200349 a 4500
001 2156556
005 2023-09-11
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ani13182832$2DOI
100 1 $aAYMÉE, L.
245 $aUnconventional sites for diagnosis of leptospirosis in bovine anicteric fetuses.$h[electronic resource]
260 $c2023
520 $aBackground: Bovine leptospirosis is an important reproductive disease and abortion is a major sign, leading to economic impacts. Due to its multifactorial etiology, the proper diagnosis of the cause of the abortion is crucial. Necropsy of the fetuses followed by molecular analysis is recommended for diagnosis, and the investigation mainly occurs in the kidneys and liver. This study aimed to analyze unconventional sites for the presence of leptospiral DNA in bovine anicteric aborted fetuses. Methods: Five fetuses of the same herd were received for necropsy and diagnosis. Conventional lipL32-PCR was performed in the fetuses? kidneys, livers, lungs, hearts, spleens, subcapsular kidney content, abomasal fluid, and in the cavity?s hemorrhagic contents. To complete the investigation, the sera of 30 cows of the herd were collected to perform the serologic screening by Microscopic Agglutination Test. In addition, six subfertile non-pregnant cows from the same herd were selected due to their low reproductive performance, and genital samples (uterine fragment and cervicovaginal mucus) and urine were collected for lipL32-PCR. PCR-positive samples were submitted to a nested PCR of the secY gene and intended for sequencing. Results: The herd presented seroreactive animals (11/30, 36.6%), all against the Sejroe serogroup, with titers between 200 and 1600. In necropsy, four fetuses showed hemorrhagic and anicteric lesions, while one fetus had no macroscopic lesions. Regarding molecular analysis, all the fetuses were positive in lipL32-PCR and the positive sites were the heart, lungs, subcapsular kidney content, thymus, kidneys, liver, and abomasal fluid. Only one fetus presented positive results in the kidney and liver, while three fetuses were positive in the abomasal fluid. Five of six cows were positive for lipL32-PCR, all being positive only in genital samples. Of the fetuses and the cows, seven sequences were obtained and all were identified as Leptospira interrogans serogroup Sejroe serovar Hardjoprajitno. Conclusions: In order to improve the diagnosis of leptospirosis in cows, it is recommended to perform a comprehensive analysis of the samples, beyond the kidneys and liver. Thus, we highly encourage testing multiple organs by PCR to investigate abortions suspected of bovine leptospirosis, particularly in anicteric fetuses.
650 $aAbortion (animals)
650 $aCattle
650 $aNecropsy
650 $aAborto
650 $aBovino
650 $aGado
650 $aLeptospirose
653 $aAbomasal fluid
653 $aLeptospiral infection
653 $aLipL32
653 $aMolecular analysis
700 1 $aDI AZEVEDO, M. I. N.
700 1 $aREIS, L.
700 1 $aMENDES, J.
700 1 $aCASTRO, F. de D. A. de
700 1 $aCARVALHO-COSTA, F. A. C.
700 1 $aSOUZA, G. N. de
700 1 $aLILENBAUM, W.
773 $tAnimals$gv. 13, 2832, 2023.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
09/05/2012 |
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Data da última atualização: |
09/05/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MÖLLER, P. A.; WENDLAND, A.; PEREIRA, H. S.; COSTA, J. G. C. da; MELO, L. C. |
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Afiliação: |
PRISCILLA AGUIAR MÖLLER, bolsista CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; HELTON SANTOS PEREIRA, CNPAF; JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF; LEONARDO CUNHA MELO, CNPAF. |
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Título: |
Reação de genótipos de feijoeiro comum a Fusarium oxysporum f. sp phaseoli. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SEMINÁRIO JOVENS TALENTOS, 5., 2011, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2011. |
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Páginas: |
p. 63. |
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Série: |
(Embrapa Arroz e Feijão. Documentos, 270). |
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Idioma: |
Português |
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Conteúdo: |
O feijoeiro comum (Phaseolus vulgaris L.) tem sido muito cultivado em áreas irrigadas, sobretudo sob o sistema de pivô central. O microclima proveniente desse sistema aliado à falta de adoção de métodos preventivos de controle e ao cultivo sucessivo na mesma área, convergem para um aumento de doenças como a murcha de fusarium, provocada pelo patógeno de solo Fusarium oxysporum f.sp. phaseoli (Fop). Foram avaliados 31 genótipos de feijoeiro comum em VCU (Valor de Cultivo e Uso); 138 em EPL (Ensaio Preliminar de Linhagens) e 54 no CER-PROAGRO (ensaio de observação da Comissão Especial de Recursos ? Programa de Garantia da Atividade Agropecuária), quanto à expressão de resistência a Fop em ensaios de campo na Embrapa Arroz e Feijão sob irrigação por pivô central. |
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Thesagro: |
Doença de planta; Feijão; Phaseolus vulgaris. |
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Categoria do assunto: |
H Saúde e Patologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/59018/1/63.pdf
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Marc: |
LEADER 01540nam a2200217 a 4500
001 1924166
005 2012-05-09
008 2011 bl uuuu u00u1 u #d
100 1 $aMÖLLER, P. A.
245 $aReação de genótipos de feijoeiro comum a Fusarium oxysporum f. sp phaseoli.$h[electronic resource]
260 $aIn: SEMINÁRIO JOVENS TALENTOS, 5., 2011, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão$c2011
300 $ap. 63.
490 $a(Embrapa Arroz e Feijão. Documentos, 270).
520 $aO feijoeiro comum (Phaseolus vulgaris L.) tem sido muito cultivado em áreas irrigadas, sobretudo sob o sistema de pivô central. O microclima proveniente desse sistema aliado à falta de adoção de métodos preventivos de controle e ao cultivo sucessivo na mesma área, convergem para um aumento de doenças como a murcha de fusarium, provocada pelo patógeno de solo Fusarium oxysporum f.sp. phaseoli (Fop). Foram avaliados 31 genótipos de feijoeiro comum em VCU (Valor de Cultivo e Uso); 138 em EPL (Ensaio Preliminar de Linhagens) e 54 no CER-PROAGRO (ensaio de observação da Comissão Especial de Recursos ? Programa de Garantia da Atividade Agropecuária), quanto à expressão de resistência a Fop em ensaios de campo na Embrapa Arroz e Feijão sob irrigação por pivô central.
650 $aDoença de planta
650 $aFeijão
650 $aPhaseolus vulgaris
700 1 $aWENDLAND, A.
700 1 $aPEREIRA, H. S.
700 1 $aCOSTA, J. G. C. da
700 1 $aMELO, L. C.
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Embrapa Arroz e Feijão (CNPAF) |
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