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| Registros recuperados : 8 | |
| 3. |  | GOULART, R de, S.; FORS, R. O.; FREIRE, L. R.; BALDANI, V. L. D. Acumulo de N em plantas de arroz cultivadas com adição de resíduos da indústria de cervejaria e inoculação de Azospirillum brasilense 245. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 31.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 15.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 13.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 10., 2014, Araxá. Fertilidade e biologia do solo: integração e tecnologias para todos: anais. Araxá: Núcleo Regional Leste da Sociedade Brasileira de Ciência do Solo, 2014. FertBio 2014.| Biblioteca(s): Embrapa Agrobiologia. |
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| 4. | | FORS, R. O.; IGNACIO, I. G.; BERBARA, R. L. L.; SAGGIN JUNIOR, O. J. Diversity of arbuscular mycorrhizal fungi in areas of sugarcane inoculation with nitrogen frixing bacteria. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. Seeion 2, Poster II.7| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | SAGGIN JUNIOR, O. J.; FORS, R. O.; ARÊAS, P. M. J.; SILVA, E. M. R. da; BERBARA, R. L. O. Seleção de fungos micorrízicos arbusculares para crescimento e nutrição fosfatada de cana-de-açúcar. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., FERTBIO 2016. Goiânia. Rumo aos novos desafios: anais. Goiânia: UFG, 2016.| Biblioteca(s): Embrapa Agrobiologia. |
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| 8. | | ARDITO, A. C. da S.; SAGGIN JUNIOR, O. J.; FORS, R. O.; COSTA, R. S. C. da; SILVA, E. M. R. da. Arbuscular mycorrhizal fungi selection for Coffea canephora Pierre ex A. Froehner Clonal cultivar conilon BRS Ouro Preto. Coffee Science, Lavras, v. 12, n. 4, p. 486-407, out./dez. 2017.| Biblioteca(s): Embrapa Agrobiologia; Embrapa Rondônia. |
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| Registros recuperados : 8 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
30/12/2019 |
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Data da última atualização: |
06/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SBARDELLA, A. P.; WELLER, M. M. D. C. A.; I. FONSECA; N. B. STAFUZZA; P. A. BERNARDES; F. F. e SILVA; SILVA, M. V. G. B.; MARTINS, M. F.; D. P. MUNARI. |
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Afiliação: |
A. P. SBARDELLA; M. M. D. C. A. WELLER; MARCOS VINICIUS GUALBERTO B SILVA, CNPGL; MARTA FONSECA MARTINS, CNPGL. |
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Título: |
Ribonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 102, n. 2, p. 1761-1767, 2019. |
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DOI: |
https://doi.org/10.3168/jds.2018-15516 |
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Idioma: |
Inglês |
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Conteúdo: |
The aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation. |
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Palavras-Chave: |
BaySeq; Crossbred dairy cow; Cuffdiff 2; EdgeR; RNA sequencing. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02292naa a2200289 a 4500
001 2117886
005 2024-02-06
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3168/jds.2018-15516$2DOI
100 1 $aSBARDELLA, A. P.
245 $aRibonucleic acid sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae.$h[electronic resource]
260 $c2019
520 $aThe aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein × Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumina Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation.
653 $aBaySeq
653 $aCrossbred dairy cow
653 $aCuffdiff 2
653 $aEdgeR
653 $aRNA sequencing
700 1 $aWELLER, M. M. D. C. A.
700 1 $aI. FONSECA
700 1 $aN. B. STAFUZZA
700 1 $aP. A. BERNARDES
700 1 $aF. F. e SILVA
700 1 $aSILVA, M. V. G. B.
700 1 $aMARTINS, M. F.
700 1 $aD. P. MUNARI
773 $tJournal of Dairy Science$gv. 102, n. 2, p. 1761-1767, 2019.
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