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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
13/03/2024 |
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Autoria: |
TENOVER, F. C.; ARBEIT, R.; ARCHER, G.; BIDDLE, J.; BYRNE, S.; GOERING, R.; HANCOCK, G.; ANN HEBERT, G.; HILL, B.; HOLLIS, R.; JARVIS, W. R.; KREISWIRTH, B.; EISNER, W.; MASLOW, J.; McDOUGAL, L. K.; MILLER, J. M.; MULLIGAN, M.; PFALLER, M. A. |
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Título: |
Comparison of traditional and molecular methods of typing isolates of Staphylococcus aureus. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 32, n. 2, p.407-415, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Fifty-nine Staphylococcus aureus isolates and 1 isolate of Staphylococcus intermedius were typed by investigators at eight institutions by using either antibiograms, bacteriophage typing, biotyping, immunoblotting, insertion sequence typing with IS257/431, multilocus enzyme electrophoresis, restriction analysis of plasmid DNA, pulsed-field or field inversion gel electrophoresis, restriction analysis of PCR-amplified coagulase gene sequences, restriction fragment length polymorphism typing by using four staphylococcal genes as probes, or ribotyping. Isolates from four well-characterized outbreaks (n = 29) and a collection of organisms from two nursing homes were mixed with epidemiologically unrelated stock strains from the Centers for Disease Control and Prevention. Several isolates were included multiple times either within or between the sets of isolates to analyze the reproducibilities of the typing systems. Overall, the DNA-based techniques and immunoblotting were most effective in grouping outbreak-related strains, recognizing 27 to 29 of the 29 outbreak-related strains; however, they also tended to include 3 to 8 epidemiologically unrelated isolates in the same strain type. Restriction fragment length polymorphism methods with mec gene-associated loci were less useful than other techniques for typing oxacillin-susceptible isolates. Phage typing, plasmid DNA restriction analysis, and antibiogram analysis, the techniques most readily available to clinical laboratories, identified 23 to 26 of 29 outbreak-related isolates and assigned 0 to 6 unrelated isolates to outbreak strain types. No single technique was clearly superior to the others; however, biotyping, because it produced so many subtypes, did not effectively group outbreak-related strains of S. aureus. MenosAbstract: Fifty-nine Staphylococcus aureus isolates and 1 isolate of Staphylococcus intermedius were typed by investigators at eight institutions by using either antibiograms, bacteriophage typing, biotyping, immunoblotting, insertion sequence typing with IS257/431, multilocus enzyme electrophoresis, restriction analysis of plasmid DNA, pulsed-field or field inversion gel electrophoresis, restriction analysis of PCR-amplified coagulase gene sequences, restriction fragment length polymorphism typing by using four staphylococcal genes as probes, or ribotyping. Isolates from four well-characterized outbreaks (n = 29) and a collection of organisms from two nursing homes were mixed with epidemiologically unrelated stock strains from the Centers for Disease Control and Prevention. Several isolates were included multiple times either within or between the sets of isolates to analyze the reproducibilities of the typing systems. Overall, the DNA-based techniques and immunoblotting were most effective in grouping outbreak-related strains, recognizing 27 to 29 of the 29 outbreak-related strains; however, they also tended to include 3 to 8 epidemiologically unrelated isolates in the same strain type. Restriction fragment length polymorphism methods with mec gene-associated loci were less useful than other techniques for typing oxacillin-susceptible isolates. Phage typing, plasmid DNA restriction analysis, and antibiogram analysis, the techniques most readily available to clinical labora... Mostrar Tudo |
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Palavras-Chave: |
Base Sequence; Epidemiologic Methods; Metodos diagnosticos; Staphylococcal Infections. |
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Thesagro: |
Epidemiologia; Staphylococcus Aureus. |
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Thesaurus Nal: |
Disease outbreaks; DNA primers; Epidemiology; Polymerase chain reaction. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03036naa a2200445 a 4500
001 1524203
005 2024-03-13
008 1994 bl uuuu u00u1 u #d
100 1 $aTENOVER, F. C.
245 $aComparison of traditional and molecular methods of typing isolates of Staphylococcus aureus.$h[electronic resource]
260 $c1994
520 $aAbstract: Fifty-nine Staphylococcus aureus isolates and 1 isolate of Staphylococcus intermedius were typed by investigators at eight institutions by using either antibiograms, bacteriophage typing, biotyping, immunoblotting, insertion sequence typing with IS257/431, multilocus enzyme electrophoresis, restriction analysis of plasmid DNA, pulsed-field or field inversion gel electrophoresis, restriction analysis of PCR-amplified coagulase gene sequences, restriction fragment length polymorphism typing by using four staphylococcal genes as probes, or ribotyping. Isolates from four well-characterized outbreaks (n = 29) and a collection of organisms from two nursing homes were mixed with epidemiologically unrelated stock strains from the Centers for Disease Control and Prevention. Several isolates were included multiple times either within or between the sets of isolates to analyze the reproducibilities of the typing systems. Overall, the DNA-based techniques and immunoblotting were most effective in grouping outbreak-related strains, recognizing 27 to 29 of the 29 outbreak-related strains; however, they also tended to include 3 to 8 epidemiologically unrelated isolates in the same strain type. Restriction fragment length polymorphism methods with mec gene-associated loci were less useful than other techniques for typing oxacillin-susceptible isolates. Phage typing, plasmid DNA restriction analysis, and antibiogram analysis, the techniques most readily available to clinical laboratories, identified 23 to 26 of 29 outbreak-related isolates and assigned 0 to 6 unrelated isolates to outbreak strain types. No single technique was clearly superior to the others; however, biotyping, because it produced so many subtypes, did not effectively group outbreak-related strains of S. aureus.
650 $aDisease outbreaks
650 $aDNA primers
650 $aEpidemiology
650 $aPolymerase chain reaction
650 $aEpidemiologia
650 $aStaphylococcus Aureus
653 $aBase Sequence
653 $aEpidemiologic Methods
653 $aMetodos diagnosticos
653 $aStaphylococcal Infections
700 1 $aARBEIT, R.
700 1 $aARCHER, G.
700 1 $aBIDDLE, J.
700 1 $aBYRNE, S.
700 1 $aGOERING, R.
700 1 $aHANCOCK, G.
700 1 $aANN HEBERT, G.
700 1 $aHILL, B.
700 1 $aHOLLIS, R.
700 1 $aJARVIS, W. R.
700 1 $aKREISWIRTH, B.
700 1 $aEISNER, W.
700 1 $aMASLOW, J.
700 1 $aMcDOUGAL, L. K.
700 1 $aMILLER, J. M.
700 1 $aMULLIGAN, M.
700 1 $aPFALLER, M. A.
773 $tJournal of Clinical Microbiology$gv. 32, n. 2, p.407-415, 1994.
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