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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/01/2022 |
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Data da última atualização: |
20/01/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEASTRO, M. O.; VILLAR-ÁLVAREZ, D.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico, São Paulo; DAVID VILLAR-ÁLVAREZ, CSIC; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, CSIC; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, CSIC. |
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Título: |
Spontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Viruses , v.13, n.12, 2021. |
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ISSN: |
1999-4915 |
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DOI: |
https://doi.org/10.3390/v13122498 |
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Idioma: |
Inglês |
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Conteúdo: |
Previous results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements. |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02107naa a2200217 a 4500
001 2139210
005 2022-01-20
008 2021 bl uuuu u00u1 u #d
022 $a1999-4915
024 7 $ahttps://doi.org/10.3390/v13122498$2DOI
100 1 $aLEASTRO, M. O.
245 $aSpontaneous mutation in the movement protein of Citrus Leprosis Virus C2, in a heterologous virus infection context, increases cell-to-cell transport and generates fitness advantage.$h[electronic resource]
260 $c2021
520 $aPrevious results using a movement defective alfalfa mosaic virus (AMV) vector revealed that citrus leprosis virus C (CiLV-C) movement protein (MP) generates a more efficient local movement, but not more systemic transport, than citrus leprosis virus C2 (CiLV-C2) MP, MPs belonging to two important viruses for the citrus industry. Here, competition experiment assays in transgenic tobacco plants (P12) between transcripts of AMV constructs expressing the cilevirus MPs, followed by several biological passages, showed the prevalence of the AMV construct carrying the CiLV-C2 MP. The analysis of AMV RNA 3 progeny recovered from P12 plant at the second viral passage revealed the presence of a mix of progeny encompassing the CiLV-C2 MP wild type (MPWT) and two variants carrying serines instead phenylalanines at positions 72 (MPS72F) or 259 (MPS259F), respectively. We evaluated the effects of each modified residue in virus replication, and cell-to-cell and long-distance movements. Results indicated that phenylalanine at position 259 favors viral cell-to-cell transport with an improvement in viral fitness, but has no effect on viral replication, whereas mutation at position 72 (MPS72F) has a penalty in the viral fitness. Our findings indicate that the prevalence of a viral population may be correlated with its greater efficiency in cell-to-cell and systemic movements.
650 $aVírus
700 1 $aVILLAR-ÁLVAREZ, D.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tViruses$gv.13, n.12, 2021.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
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Registro |
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URL |
Voltar
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| Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
16/07/1999 |
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Data da última atualização: |
16/07/1999 |
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Autoria: |
WESTERHOF, R.; VILELA, L.; AYARZA, M. A.; ZECH, W. |
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Título: |
Characterizing labile and stable nitrogen. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: THOMAS, R.; AYARZA, M.A., ed. Sustainable land management for the oxisols of the Latin American savannas: dynamics of soil organic matter and indicators of soil quality. Cali: CIAT, 1999. |
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Páginas: |
p.141-146. |
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Idioma: |
Inglês |
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Conteúdo: |
The permanganate oxidation procedure is easily performed and may help monitor large areas for their soil organic matter (SOM) quality and nitrogen (N) availability. Knowledge of the chemical characteristics of fractions that are lost or remain after oxidation will help explain results obtained by this method. We found that amino acid N contributed 22.75% to the permanganate-extractable N fraction (PEN) and amino sugar N 9.1%, but the remaining 68.2% of the PEN was of "unknown" N. Stable N had lower amounts of amino acid N (15.5%) and amino sugar N (6.2%). Permanganate-extractable N and stable N may be spatially separated, that is, PEN is found outside microaggregates, whereas stable N is inside, where it is protected from permanganate extraction and probably also from microbial breakdown. Together with physically protected N, the stable N fraction also contained an important amount of chemically recalcitrant, not-extractable, "unknown" N with a chemical composition that is hypothesized to be different from that of the permanganate-extractable "unknown" N. |
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Thesagro: |
Cerrado; Mineralização; Nitrogênio; Oxisol; Uso da Terra. |
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Thesaurus NAL: |
Ferralsols; land use; mineralization; nitrogen. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01881naa a2200277 a 4500
001 1545406
005 1999-07-16
008 1999 bl uuuu u00u1 u #d
100 1 $aWESTERHOF, R.
245 $aCharacterizing labile and stable nitrogen.
260 $c1999
300 $ap.141-146.
520 $aThe permanganate oxidation procedure is easily performed and may help monitor large areas for their soil organic matter (SOM) quality and nitrogen (N) availability. Knowledge of the chemical characteristics of fractions that are lost or remain after oxidation will help explain results obtained by this method. We found that amino acid N contributed 22.75% to the permanganate-extractable N fraction (PEN) and amino sugar N 9.1%, but the remaining 68.2% of the PEN was of "unknown" N. Stable N had lower amounts of amino acid N (15.5%) and amino sugar N (6.2%). Permanganate-extractable N and stable N may be spatially separated, that is, PEN is found outside microaggregates, whereas stable N is inside, where it is protected from permanganate extraction and probably also from microbial breakdown. Together with physically protected N, the stable N fraction also contained an important amount of chemically recalcitrant, not-extractable, "unknown" N with a chemical composition that is hypothesized to be different from that of the permanganate-extractable "unknown" N.
650 $aFerralsols
650 $aland use
650 $amineralization
650 $anitrogen
650 $aCerrado
650 $aMineralização
650 $aNitrogênio
650 $aOxisol
650 $aUso da Terra
700 1 $aVILELA, L.
700 1 $aAYARZA, M. A.
700 1 $aZECH, W.
773 $tIn: THOMAS, R.; AYARZA, M.A., ed. Sustainable land management for the oxisols of the Latin American savannas: dynamics of soil organic matter and indicators of soil quality. Cali: CIAT, 1999.
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