| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
22/03/2007 |
|
Data da última atualização: |
25/05/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
FIGUEIREDO, J. E. F.; DEPAOLI, H. C.; COELHO, V. T. da S.; CASELA, C. R.; FERREIRA, A. da S.; GUIMARAES, C. T.; GOMES, E. A.; BRESSAN, W. |
|
Afiliação: |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; ELIANE APARECIDA GOMES, CNPMS. |
|
Título: |
Genetic diversity among Colletotrichum sublineolum pathotypes isolated from sorghum (Sorghum bicolor). |
|
Ano de publicação: |
2006 |
|
Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 5, n. 2, p. 304-318, 2006. |
|
DOI: |
http://dx.doi.org/10.18512/1980-6477/rbms.v5n2p304-318 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Sorghum anthracnose, caused by Colletotrichum sublineolum is a serious disease in Brazil. The fungus is highly variable pathogenically with many physiological races. The present study was made to evaluate the usefulness of molecular methods (SDS-PAGE, RAPD, ARDRA and rDNA sequencing) for identifying C. sublineolum pathotypes isolated from sorghum. Although protein profile (SDS-PAGE) showed a relative low level of polymorphism, differences could be observed due to presence/absence of some polypeptides as well as to intensity of some bands among pathotypes. DNA profile of arbitrarily amplified sequences (RAPD) using sixteen random primers revealed a high degree of polymorphism while amplified rDNA (ITS region and 18S gene) digested with six different restriction enzymes (ARDRA) showed similar patterns. Nucleotide sequencing analysis of the complete ITS region and partial sequencing of 18S rRNA gene were useful for revealing genetic differences among the five pathotypes. In the present study, RAPD and rDNA sequencing were the most profitable methods for identifying C. sublineolum pathotypes. A antracnose do sorgo, causada por Colletotrichum sublineolum, é séria doença, no Brasil. O fungo é altamente variável do ponto de vista patogênico e várias raças têm sido noticiadas. Este estudo foi realizado com o objetivo de avaliar a utilidade de métodos moleculares (SDS-PAGE, RAPD, ARDRA e seqüenciamento de rDNA) para identificar patótipos de C. sublineolum isolados do sorgo. Embora o perfil protéico (SDS-PAGE) tenha mostrado baixo grau de polimorfismo, foi possível observar diferenças devido à presença/ausência de alguns polipeptídios, bem como diferenças quanto à intensidade de algumas bandas. O perfil de DNA amplificado arbitrariamente (RAPD), usando dezeseis primers aleatórios, revelou elevado grau de polimorfismo entre os patótipos, enquanto que o produto da amplificação de rDNA (região ITS e gene 18S), digerido com seis enzimas de restrição diferentes (ARDRA), apresentou padrão similar. Análise de nucleotídeos da seqüência completa da região ITS e do seqüenciamento parcial do gene 18S rRNA revelaram diferenças genéticas entre os cinco patótipos. Entre os métodos testados no presente estudo, RAPD e seqüenciamento de rDNA foram mais efetivos para identificar patótipos de C. sublineolum. MenosSorghum anthracnose, caused by Colletotrichum sublineolum is a serious disease in Brazil. The fungus is highly variable pathogenically with many physiological races. The present study was made to evaluate the usefulness of molecular methods (SDS-PAGE, RAPD, ARDRA and rDNA sequencing) for identifying C. sublineolum pathotypes isolated from sorghum. Although protein profile (SDS-PAGE) showed a relative low level of polymorphism, differences could be observed due to presence/absence of some polypeptides as well as to intensity of some bands among pathotypes. DNA profile of arbitrarily amplified sequences (RAPD) using sixteen random primers revealed a high degree of polymorphism while amplified rDNA (ITS region and 18S gene) digested with six different restriction enzymes (ARDRA) showed similar patterns. Nucleotide sequencing analysis of the complete ITS region and partial sequencing of 18S rRNA gene were useful for revealing genetic differences among the five pathotypes. In the present study, RAPD and rDNA sequencing were the most profitable methods for identifying C. sublineolum pathotypes. A antracnose do sorgo, causada por Colletotrichum sublineolum, é séria doença, no Brasil. O fungo é altamente variável do ponto de vista patogênico e várias raças têm sido noticiadas. Este estudo foi realizado com o objetivo de avaliar a utilidade de métodos moleculares (SDS-PAGE, RAPD, ARDRA e seqüenciamento de rDNA) para identificar patótipos de C. sublineolum isolados do sorgo. Embora o ... Mostrar Tudo |
|
Palavras-Chave: |
ARDRA; RAPD; SDS-PAGE; seqüenciamento de rDNA. |
|
Thesagro: |
Antracnose; Doença de planta; Sorghum Bicolor. |
|
Thesaurus Nal: |
Colletotrichum sublineolum. |
|
Categoria do assunto: |
H Saúde e Patologia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35275/1/Genetic-diversity-2.pdf
|
|
Marc: |
LEADER 03338naa a2200313 a 4500
001 1490492
005 2018-05-25
008 2006 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.18512/1980-6477/rbms.v5n2p304-318$2DOI
100 1 $aFIGUEIREDO, J. E. F.
245 $aGenetic diversity among Colletotrichum sublineolum pathotypes isolated from sorghum (Sorghum bicolor).$h[electronic resource]
260 $c2006
520 $aSorghum anthracnose, caused by Colletotrichum sublineolum is a serious disease in Brazil. The fungus is highly variable pathogenically with many physiological races. The present study was made to evaluate the usefulness of molecular methods (SDS-PAGE, RAPD, ARDRA and rDNA sequencing) for identifying C. sublineolum pathotypes isolated from sorghum. Although protein profile (SDS-PAGE) showed a relative low level of polymorphism, differences could be observed due to presence/absence of some polypeptides as well as to intensity of some bands among pathotypes. DNA profile of arbitrarily amplified sequences (RAPD) using sixteen random primers revealed a high degree of polymorphism while amplified rDNA (ITS region and 18S gene) digested with six different restriction enzymes (ARDRA) showed similar patterns. Nucleotide sequencing analysis of the complete ITS region and partial sequencing of 18S rRNA gene were useful for revealing genetic differences among the five pathotypes. In the present study, RAPD and rDNA sequencing were the most profitable methods for identifying C. sublineolum pathotypes. A antracnose do sorgo, causada por Colletotrichum sublineolum, é séria doença, no Brasil. O fungo é altamente variável do ponto de vista patogênico e várias raças têm sido noticiadas. Este estudo foi realizado com o objetivo de avaliar a utilidade de métodos moleculares (SDS-PAGE, RAPD, ARDRA e seqüenciamento de rDNA) para identificar patótipos de C. sublineolum isolados do sorgo. Embora o perfil protéico (SDS-PAGE) tenha mostrado baixo grau de polimorfismo, foi possível observar diferenças devido à presença/ausência de alguns polipeptídios, bem como diferenças quanto à intensidade de algumas bandas. O perfil de DNA amplificado arbitrariamente (RAPD), usando dezeseis primers aleatórios, revelou elevado grau de polimorfismo entre os patótipos, enquanto que o produto da amplificação de rDNA (região ITS e gene 18S), digerido com seis enzimas de restrição diferentes (ARDRA), apresentou padrão similar. Análise de nucleotídeos da seqüência completa da região ITS e do seqüenciamento parcial do gene 18S rRNA revelaram diferenças genéticas entre os cinco patótipos. Entre os métodos testados no presente estudo, RAPD e seqüenciamento de rDNA foram mais efetivos para identificar patótipos de C. sublineolum.
650 $aColletotrichum sublineolum
650 $aAntracnose
650 $aDoença de planta
650 $aSorghum Bicolor
653 $aARDRA
653 $aRAPD
653 $aSDS-PAGE
653 $aseqüenciamento de rDNA
700 1 $aDEPAOLI, H. C.
700 1 $aCOELHO, V. T. da S.
700 1 $aCASELA, C. R.
700 1 $aFERREIRA, A. da S.
700 1 $aGUIMARAES, C. T.
700 1 $aGOMES, E. A.
700 1 $aBRESSAN, W.
773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 5, n. 2, p. 304-318, 2006.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Unidades Centrais. |
|
Data corrente: |
23/08/2001 |
|
Data da última atualização: |
13/09/2004 |
|
Autoria: |
VIEIRA, S. R.; CASTRO, O. M. de; TOPP, G. C. |
|
Título: |
Spatial Variability of Some Soil Physical Properties in Three Soils of Sao Paulo, Brazil. |
|
Ano de publicação: |
1992 |
|
Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.27, n.2, p.333-341, fev.1992 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Volumetric core samples and loose soil samples were collected at 1 m interval in 10 m long transects, on three major soil groups in São Paulo, Brazil. The soil cores were used for soil desorption curves and the bulk density, and the loose soil was used for particle size analysis. The parameters obtained from the soil water retention model, bulk density and particle size values were submitted to geostatistical analysis to study the spatial variability through the examination of semivariograms and cross-semivariogram. The results showed that the scale of variation changes from soil to soil, with maximum variation for the Podzolic Soil with clay surface texture, from the northeast part of the state. For this soil also, the spatial autocorrelation was the most expressive. The variability of the soil desorption parameters was related to the particle size parameters. The geostatistical analysis results allowed establishing sample spacing for the soils studied, to allow for estimation at any finer spacing, with no bias and minimum variance. |
|
Palavras-Chave: |
soil hydraulic conductivity; soil water desorption. |
|
Thesaurus NAL: |
bulk density; particle size; soil; soil water retention. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/20622/1/pab16_fev_92.pdf
|
|
Marc: |
LEADER 01697naa a2200217 a 4500
001 1105546
005 2004-09-13
008 1992 bl uuuu u00u1 u #d
100 1 $aVIEIRA, S. R.
245 $aSpatial Variability of Some Soil Physical Properties in Three Soils of Sao Paulo, Brazil.
260 $c1992
520 $aVolumetric core samples and loose soil samples were collected at 1 m interval in 10 m long transects, on three major soil groups in São Paulo, Brazil. The soil cores were used for soil desorption curves and the bulk density, and the loose soil was used for particle size analysis. The parameters obtained from the soil water retention model, bulk density and particle size values were submitted to geostatistical analysis to study the spatial variability through the examination of semivariograms and cross-semivariogram. The results showed that the scale of variation changes from soil to soil, with maximum variation for the Podzolic Soil with clay surface texture, from the northeast part of the state. For this soil also, the spatial autocorrelation was the most expressive. The variability of the soil desorption parameters was related to the particle size parameters. The geostatistical analysis results allowed establishing sample spacing for the soils studied, to allow for estimation at any finer spacing, with no bias and minimum variance.
650 $abulk density
650 $aparticle size
650 $asoil
650 $asoil water retention
653 $asoil hydraulic conductivity
653 $asoil water desorption
700 1 $aCASTRO, O. M. de
700 1 $aTOPP, G. C.
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.27, n.2, p.333-341, fev.1992
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
