| |
|
|
| Registros recuperados : 54 | |
| 41. |  | VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|    |
| 42. | | DALTRO, P. S.; ALVES, A. A. C.; VILARINHOS, A. D.; VIEIRA, L. de J.; SOUZA, W. F.; SANTOS, V. J. dos. Avaliação da diversidade genética de genótipos de mandioca tolerantes a seca utilizando marcadores microssatélites. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 2., 2008, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2008. RESUMO_0933.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 43. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; SILVEIRA, T. C. da; ALVES, A. A. C.; SOUZA, F. V. D. Conservação e longevidade de pólen de acessos de Manihot esculenta. In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. 1 CD ROM. Melhoramento genético. Resumo n. 266.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 44. | | SILVEIRA, T. C. da; VIEIRA, L. de J.; LEDO, C. A. da S.; FERREIRA, C. F.; OLIVEIRA, G. A. F.; OLIVEIRA, E. J. de. Identificação de genótipos de Manihot sp. com alelos de resistência ao vírus do Mosaico Africano. In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. 1 CD ROM. Fitosanidade. Resumo n. 185.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 45. | | VIEIRA, L. de J.; ALVES, A. A. C.; VILARINHOS, A. D.; DALTRO, P. S.; SOUZA, W. F.; SILVA, P. H. da; SANTOS, V. J. dos. Seleção de híbridos de mandioca para resistência a vírus de mosaico africano assistida por marcadores moleculares. In: JORNADA CIENTÍFICA EMBRAPA MANDIOCA E FRUTICULTURA TROPICAL, 2., 2008, Cruz das Almas. Anais... Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 2008. RESUMO_0733.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 46. | | VIEIRA, L. de J.; SAMPAIO, E. dos S.; CONCEIÇÃO, J. Q. da; SANTOS, L. B. P. R. dos; LEDO, C. A. da S.; SOUZA, F. V. D. Uso de alporquia para propagação de espécies de Manihot de difícil enraizamento. In: CONGRESSO BRASILEIRO DE MANDIOCA, 16.; CONGRESSO LATINO-AMERICANO E CARIBENHO DE MANDIOCA, 2015, Foz do Iguaçu. Integração: segurança alimentar e geração de renda: anais. Foz do Iguaçu: SBM, 2015. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 47. | | SILVEIRA, T. C. da; VIEIRA, L. de J.; SOUZA, F. V. D.; LEDO, C. A. da S.; ALVES, A. A. C. Viabilidade de pólen de sub-espécies de Manihot esculenta. In: CONGRESSO BRASILEIRO DE MANDIOCA, 14.; FEIRA BRASILEIRA DA MANDIOCA, 1., 2011, Maceió. Mandioca: fonte de alimento e energia: anais. Maceió: ABAM: SBM, 2011. 1 CD ROM. Propagação. Resumo n. 249.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 48. | | VIEIRA, L. de J.; SOARES, T. L.; ROSSI, M. L.; ALVES, A. A. C.; SANTOS, F. de A. R. dos; SOUZA, F. V. D. Viability, production and morphology of pollen grains for diff erent species in the genus Manihot (Euphorbiaceae). Acta Botanica Brasilica, v. 26, n. 2, p. 350-356, 2012.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 49. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, A. da S.; CARVALHO, M. de J. da S.; SANTOS, E. B.; ALVES, A. A. C.; ELLIS, D. Cryopreservation of Hamilin sweet orange [(Citrus sinensis (L.) Osbeck)] embryogenic calli using a modified aluminum cryo-plate technique. Scientia Horticulturae, v.224, p.302-305, October ,2017.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 50. | | SOUZA, F. V. D.; KAYA, E.; VIEIRA, L. de J.; SOUZA, E. H. de; AMORIM, V. B. de O.; SKOGERBOE, D.; MATSUMOTO, T.; ALVES, A. A. C.; LEDO, C. A. da S.; JENDEREK, M. M. Droplet-vitrification and morphohistological studies of cryopreserved shoot tips of cultivated and wild pineapple genotypes. Plant Cell, Tissue and Organ Culture, v.123, n.2, nov. 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 51. | | VIEIRA, L. de J.; PASSOS, R. R.; OLIVIERA, T. da S.; SANTANA, J. R. F. de; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação da longevidade de grãos de pólen de de Manihot esculenta spp. flabellifolia por teste colorimétrico e fluorescência. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 52. | | OLIVEIRA, T. da S.; VIEIRA, L. de J.; PASSOS, R. R.; SANTANA, J. R. F. de; MARTINS, M. L. L.; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação da receptividade de estigmas de Manihot esculenta spp. flabellifolia. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
|  |
| 53. | | VIEIRA, L. de J.; OLIVEIRA, T. da S.; PASSOS, R. R.; SANTANA, J. R. F. de; LEDO, C. A. da S.; ALVES, A. A. C.; SOUZA, F. V. D. Avaliação de métodos para desidratação de grãos de pólen de M. esculenta. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 2., 2012, Belém, PA. Anais... Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2012. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| 54. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; VIDAL, A. M.; ALVES, A. A. C.; SOUZA, A. da S.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation. African Journal of Biotechnology, February, v.14, n.5, p. 419-424, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
| |
| Registros recuperados : 54 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
|
Data corrente: |
30/10/2015 |
|
Data da última atualização: |
30/05/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
B - 1 |
|
Autoria: |
VIEIRA, L. de J.; SANTANA, J. R. F. de; ALVES, A. A. C.; LEDO, C. A. da S.; SOUZA, F. V. D. |
|
Afiliação: |
LÍVIA DE JESUS VIEIRA, UEFS; JOSÉ RANIERE FERREIRA DE SANTANA, UEFS; ALFREDO AUGUSTO CUNHA ALVES, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
|
Título: |
Use of aniline blue stain to observing pollen tubes development in different Manihot Mill. species. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
African Journal of Agricultural Research, v. 10, n. 15, p. 1805-1809, 9 April, 2015. |
|
Idioma: |
Inglês |
|
Conteúdo: |
The lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability. MenosThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The ... Mostrar Tudo |
|
Thesagro: |
Mandioca; Melhoramento vegetal; Recurso genético. |
|
Thesaurus NAL: |
Breeding methods; Cassava; Genetic resources. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02645naa a2200241 a 4500
001 2027633
005 2023-05-30
008 2015 bl uuuu u00u1 u #d
100 1 $aVIEIRA, L. de J.
245 $aUse of aniline blue stain to observing pollen tubes development in different Manihot Mill. species.$h[electronic resource]
260 $c2015
520 $aThe lack of a reliable test to evaluate pollen viability in species of the genus Manihot is one of the primary limitations for cassava genetic improvement. Thus, the objective of the present study was to apply aniline blue stain to observing pollen tubes development in different Manihot Mill. species, in order to estimate the crossability, pollen germination time, pollen tube size and pollen longevity. Pollen grains from 2 accessions of the cultivated species M. esculenta spp. esculenta and 4 accessions of 3 wild species (M. esculenta ssp. flabellifolia (Pohl) Cif, M. anomala Pohl, and M. irwinii D. J. Roger and Appan) were used. To visualize the pollen tubes, pollinated stigmas was deposited on a glass slide with 3 drops of aniline blue stain and transferred to an optical fluorescence microscope, where they were visualized and photographed using the 10x ocular lens. The pollen crossability rate was estimated by the ercentage of germinated pollen, with well-developed pollen tubes in relation to the total number of pollen grains counted per stigma. The experimental design was entirely random with 3 repetitions, each repetition consisting of 100 pollen grains. In addition to the estimation of crossability, the germination time after pollination, pollen tube size, and pollen longevity were also determined to the accession FLA 029V-01. Significant difference was observed between the crossings. Pollen tubes developed after 45 min of contact between the pollen and the stigma. The average pollen tube size 24 h after pollination was 2.9 mm. The studies examining pollen longevity showed that germination did not occur when the pollen grains were evaluated 24 h after anthesis. Thus, the use of aniline blue stain can be considered an effective method for observing pollen tubes development and studying the pollen morphology, longevity, viability and Manihot crossability.
650 $aBreeding methods
650 $aCassava
650 $aGenetic resources
650 $aMandioca
650 $aMelhoramento vegetal
650 $aRecurso genético
700 1 $aSANTANA, J. R. F. de
700 1 $aALVES, A. A. C.
700 1 $aLEDO, C. A. da S.
700 1 $aSOUZA, F. V. D.
773 $tAfrican Journal of Agricultural Research$gv. 10, n. 15, p. 1805-1809, 9 April, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
