Data/hora: 24/06/2024 18:42:24

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 27/10/2015
Data da última atualização: | 18/05/2023
Autoria: | VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; SOUZA, A. da S.; LEDO, C. A. da S.
Afiliação: | Á. M. VIDAL, UFRB; L. J. VIEIRA; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF.
Título: | Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.
Ano de publicação: | 2015
Fonte/Imprenta: | Genetics and Molecular Research, v.14, n. 3, p. 7759-7770, 2015.
ISSN: | 1676-5680
Idioma: | Inglês
Conteúdo: | Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.
Palavras-Chave: | Marcado molecular.
Thesagro: | Anatomia vegetal; Banco de germoplasma; Mandioca; Meristema.
Thesaurus Nal: | Cassava; Genetic markers; Germplasm.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (30885-1) - UPC - AP - DD - Publicação digital -  -  - DIS