Data/hora: 16/06/2024 02:19:08

Biblioteca(s): | Embrapa Café.
Data corrente: | 08/01/2024
Data da última atualização: | 08/01/2024
Autoria: | CARVALHO, K.; CAMPOS, M. K. F. de; PEREIRA, L. F. P.; VIEIRA, L. G. E.
Afiliação: | K. CARVALHO, INSTITUTO AGRONÔMICO DO PARANÁ; M. K. F. DE CAMPOS, INSTITUTO AGRONÔMICO DO PARANÁ; LUIZ FILIPE PROTASIO PEREIRA, CNPCa; L. G. E. VIEIRA, INSTITUTO AGRONÔMICO DO PARANÁ.
Título: | Reference gene selection for real-time quantitative polymerase chain reaction normalization in "Swingle" citrumelo under drought stress.
Ano de publicação: | 2010
Fonte/Imprenta: | Analytical Biochemistry, v. 402, n. 2, p. 197-199, 2010.
DOI: | http://dx.doi.org/10.1016/j.ab.2010.03.038
Idioma: | Português
Conteúdo: | We describe the first systematic evaluation of reference genes for use in real-time quantitative polymerase chain reaction (qPCR) for water deficit stress studies in the citrus rootstock "Swingle" citrumelo. The expression levels of seven reference genes cyclophilin (CYP), cathepsin (CtP), actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), elongation factor 1 alpha (EF1 alpha), beta-tubulin (TUB), and ADP ribosylation factor (ADP) during drought stress were tested using geNorm and NormFinder programs. Results from four experimental conditions indicated that EF1 alpha and ADP were the most stable reference genes. Relative expression levels of Delta 1-pyrroline-5-carboxylate synthetase (P5CS) was used for reference gene validation.
Thesaurus Nal: | Cyclophilins; Plant selection guides; Quantitative polymerase chain reaction; Water stress.
Categoria do assunto: | --
Registro original: | Embrapa Café (CNPCa)
Exemplares: | CNPCa - (1776-1) - UPC - AP - DD -  -  -  - DIS