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Registro Completo |
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Biblioteca(s): |
Embrapa Hortaliças. |
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Data corrente: |
31/03/1993 |
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Data da última atualização: |
05/03/2024 |
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Autoria: |
KITAJIMA, E. W.; RESENDE, R. de O.; AVILA, A. C. de; GOLDBACH, R. W.; PETERS, D. |
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Afiliação: |
ANTONIO CARLOS DE AVILA, CNPH. |
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Título: |
Immuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Journal of Virological Methods, v. 38, n. 3, p. 313-322, Aug./Sept. 1992. |
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DOI: |
https://doi.org/10.1016/0166-0934(92)90076-P |
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Idioma: |
Inglês |
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Conteúdo: |
Tomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not. |
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Palavras-Chave: |
Deteccao; Immunogold; Imunoflorescencia; Labelling; TSWV; Vira-Cabeca. |
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Thesaurus Nal: |
detection; Tomato spotted wilt virus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02285naa a2200277 a 4500
001 1749564
005 2024-03-05
008 1992 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/0166-0934(92)90076-P$2DOI
100 1 $aKITAJIMA, E. W.
245 $aImmuno-electron microscopical detection of tomato spotted wilt virus and its nucleocapsids in crude plant extracts.$h[electronic resource]
260 $c1992
520 $aTomato spotted wilt virus (TSWV) particles were identified in thin sections and in crude extracts of leaves from plants infected with different TSWV isolates, using gold labelled protein A and antibodies prepared against purified virus particles or against nucleocapsid preparations. In addition, both in thin sections and in dip preparations aggregates were detected using either goldlabelled antiserum. These aggregates were not detected using antisera against envelope proteins or against the non-structural protein NSs. They were the only detectably labelled material in preparations made from plants infected by morphologically defective isolates of TSWV, i.e. isolates that did not produce enveloped particles. The aggregates were discerned in dip preparations as cloudy amorphous structures having a cottonwool boll-like appearance. These ‘cotton bolls’ were interpreted as being the in vitro structures of the nucleocapsid aggregates occurring in plant cells infected by isolates maintained by mechanical inoculation and are the only structures in cells infected by morphologically defective isolates (Ie, 1982; Kitajima et al., 1992). The detection of the ‘cotton boll-like’ structures in crude extracts provides an adequate and rapid method to establish the presence of nucleocapsid aggregates in cells of infected plants, irrespective of whether they are infected by morphologically defective isolates or not.
650 $adetection
650 $aTomato spotted wilt virus
653 $aDeteccao
653 $aImmunogold
653 $aImunoflorescencia
653 $aLabelling
653 $aTSWV
653 $aVira-Cabeca
700 1 $aRESENDE, R. de O.
700 1 $aAVILA, A. C. de
700 1 $aGOLDBACH, R. W.
700 1 $aPETERS, D.
773 $tJournal of Virological Methods$gv. 38, n. 3, p. 313-322, Aug./Sept. 1992.
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| 1. |  | NASCIMENTO FILHO, I. do; VIECELI, N. C.; CARDOSO, E. M.; LOVATEL, E. R.; GONZATTI, C. F.; MARZOTTO, J. A.; MONTEZANO, D. G.; SPECHT, A. Two generations of fall armyworm (Lepidoptera: Noctuidae) contamination by Di-n-Butylphthalate. Journal of Toxicology and Environmental Health, Part A: Current Issues, v. 76, n. 16, p. 973-977, 2013.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
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