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| 163. |  | VIDAL, M. S.; TEIXEIRA, K. R. dos S.; SOARES, C. P.; FERNANDES, S. M. A.; SILVA, R. J. Seleção de mutantes de gluconacetobacter diazotrophicus defectivos na síntese de ácidos Orgãnicos In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25.,2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Bras. de Microbiologia, 2009. 01 01 de 01| Biblioteca(s): Embrapa Agrobiologia. |
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| 166. | | FERREIRA, V. L.; GALISA, P. S.; MACEDO, A. V. M.; VIDAL, M. S.; ARAÚJO, J. L S. Uso de diferentes fontes de carbono no Isolamento de genes em Gluconacetobacter diazotrophicus estirpe PAL 5 In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009. 01 01| Biblioteca(s): Embrapa Agrobiologia. |
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| 168. | | SOARES, C. de P.; TERRA, L. A.; TADRA-SFEIR, M. Z.; VIDAL, M. S.; BALDANI, J. I. Transcriptome analysis of Gluconacetobacter diazotrophicus PAL5 strain in response to iron. In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION, 16., LATINAMERICAN WORKSHOP OF PGPR, 4., RELARE, 19., 2018, Foz do Iguaçu. Book of abstract....Foz do Iguaçu: Emprapa, 2018. p. 50 Session 4, Poster IV.1| Biblioteca(s): Embrapa Agrobiologia. |
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| 169. | | SILVA, J. R. da; GALISA, P. de S.; GONÇALO, T. P.; VIDAL, M. S.; ARAÚJO, J. L. S. de. Uso de marcador molecular na avaliação de genótipos de feijão com capacidade de fixação biológica de N. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2009. p.32| Biblioteca(s): Embrapa Agrobiologia. |
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| 170. | | GALVÃO, P. Q.; RODRIGUES, E. P.; ROUWS, L. F. M.; ARAUJO, J. L. S. de; VIDAL, M. S.; BALDANI, J. I. Analysis of Gluconacetobacter diazotrophicus PAL5 nitB mutant. In: INTERNATIONAL SYMPOSIUM ON BIOLOGICAL NITROGEN WITH NON-LEGUMES, 12.; INTERNATIONAL INCT SYMPOSIUM ON NITROGEN BIOLOGICAL FIXATION, 2., 03 a 8 de outubro, 2010, Búzios, RJ. Book of abstracts... Seropédica: Embrapa Agrobiologia, 2010. SI 13| Biblioteca(s): Embrapa Agrobiologia. |
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| 171. | | GALVÃO, P. G.; MELO, L. H. V. de; GITAHY, P. M.; VIDAL, M. S.; SILVA, F. R. da; SIMÕES-ARAÚJO, J. L.; BALDANI, J. I. Ajuste metodológico para construção de bibliotecas e seqüenciamento de plasmídeos de Bacillus thuringiensis subsp. kurstaki estirpe S76. In: CONGRESSO BRASILEIRO DE GENÉTICA, 54., 2008, Salvador. Anais... Salvador: SBG, 2008. p. 78.| Biblioteca(s): Embrapa Agrobiologia. |
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| 174. | | CORDEIRO, T. F.; GALVÃO, P. G.; GUEDES, H.; MENESES, C. H. G.; VIDAL, M. S.; ARAÚJO, J. L. S. de; BALDANI, J. I. Avaliação da expressão gênica em Gluconacetobacter diazotrophicus durante a interação com cana-de-açúcar. In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009, Seropédica. Ciência no Brasil: desafios, avanços e aplicações. Seropédica: Embrapa Agrobiologia, 2009. 6| Biblioteca(s): Embrapa Agrobiologia. |
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| 175. | | CORDEIRO, T. F.; GALVÃO, P. G.; GUEDES, H.; MENESES, C. H. G.; VIDAL, M. S.; ARAÚJO, J. L. S.; BALDANI, J. I. Avaliação da Expressão gênica em Gluconacetobacter diazotropicus durante a interação com cana-de-açúcar In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009. Seropédica: Embrapa Agrobiologia, 2009 01 01| Biblioteca(s): Embrapa Agrobiologia. |
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| 176. | | GALISA, P. S.; ALVES, G. C.; FERREIRA, V. L.; GONÇALO, T. P.; VIDAL, M. S.; BALDANI, J. I.; ARAUJO, J. L. S. de. Avaliação do efeito do caldo de cana-de-açúcar no perfil de expressão Gênica da bactéria gluconacetobacter diazotrophicus In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 25.,2009, Porto de Galinhas, PE. Anais... [São Paulo]: Sociedade Bras. de Microbiologia, 2009. 01 p. 01 de 01| Biblioteca(s): Embrapa Agrobiologia. |
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| 177. | | NASCIMENTO, W. B. A.; SANTOS, C. M. dos; CESAR, M. J. da S. C.; BALDANI, J. I.; VIDAL, M. S.; SCHWAB, S. Avaliação do potencial de novos isolados de bactérias do gênero Sphingomonas para uso como inoculante na cultura da cana-de-açúcar. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER 21., 2021, Seropédica. A transversalidade da ciência, tecnologia e inovações para o planeta: caderno de resumos. Seropédica: Embrapa Agrobiologia, 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 178. | | BALDANI, J. I.; VIDEIRA, S. S.; QUESSADA, D.; BALDANI, V. L. D.; VIDAL, M. S.; SCHWAB, S.; ARAUJO, J. L. S. de; URQUIAGA, S. BNF in the bioenergy Pennisetum purpureum In: SYMPOSIUM ON BIOLOGICAL NITROGEN FIXATION WITH NON-LEGUMES, 15.; SYMPOSIUM OF THE EUROPEAN NITROGEN FIXATION CONFERENCE, 12., 2016, Hungary. Book of abstracts. Hungary: Biological Research Center, 2016.| Biblioteca(s): Embrapa Agrobiologia. |
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| 179. | | FERREIRA, V. de L.; GALISA, P. de S.; SCHWAB, S.; VIDAL, M. S.; BALDANI, J. I.; ARAUJO, J. L. S. de. Caracterização da função de MatE em Gluconacetobacter diazotrophicus. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 10., 18 a 24 de outubro de 2010, Seropédica. Ciência para o Desenvolvimento Sustentável: anais... Seropédica: Embrapa Agrobiologia, 2010.| Biblioteca(s): Embrapa Agrobiologia. |
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| 180. | | CARVALHO, P. A.; MENESES, C. H. S. G.; ROUWS, L. F. M.; ARAÚJO, J. S. A.; VIDAL, M. S.; BALDANI, J. I. caracterização funcional de uma provável B-1,4- endoglucanase de gluconacetobacter dia Diazotrophicus PAL5T In: SEMANA CIENTÍFICA JOHANNA DOBEREINER, 9., 19 a 23 de outubro de 2009. Seropédica: Embrapa Agrobiologia, 2009 01 01| Biblioteca(s): Embrapa Agrobiologia. |
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Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
18/05/2023 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
SHIOSE, L.; VIDAL, M. S.; HERINGER, A. S; VESPOLI, L. S.; SILVEIRA, V.; BALDANI, J. I.; JESUS, E. da C. |
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Afiliação: |
LUMI SHIOSE, UFRJ; MARCIA SOARES VIDAL, CNPAB; ANGELO S. HERINGER, UENF; LUCIANO S. VESPOLI, UENF; VANILDO SILVEIRA, UENF; JOSE IVO BALDANI, CNPAB; EDERSON DA CONCEICAO JESUS, CNPAB. |
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Título: |
Proteomic analysis of common bean (Phaseolus vulgaris L.) leaves showed a more stable metabolism in a variety responsive to biological nitrogen fixation. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Symbiosis, Published: 10 May 2023. |
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ISSN: |
0334-5114 |
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DOI: |
https://doi.org/10.1007/s13199-023-00921-z |
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Idioma: |
Inglês |
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Conteúdo: |
The common bean (Phaseolus vulgaris L.) can supply part of its N demand through biological nitrogen fixation (BNF) in symbiosis with rhizobia. However, the responses to inoculation and BNF's contribution to plant nutrition vary with the plant genotype. Here, we examined the proteome of two common bean genotypes (Ouro Negro and BRS Radiante) to determine shifts in leaf metabolism with two N sources: mineral N and inoculation with Rhizobium tropici CIAT899 (= SEMIA 4077 = BR 322). Data are available via ProteomeXchange with the identifier PXD034817. Through UPLC-MS/MS analysis, we iden- tified 917 proteins, 338 of which are differentially expressed. Here we focus on the 64 and 22 proteins differentially expressed in BRS Radiante and Ouro Negro, respectively, when comparing the proteome profiles of inoculated and N-fertilized plants. Different pathways in P. vulgaris were affected positively and negatively in BRS Radiante during symbiosis: glutathione metabolism, cysteine and methionine metabolism, phenylpropanoid biosynthesis, sulfur metabolism, peroxisome pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, carbon metabolism, and phagosome were the identified pathways. However, only three pathways were affected in Ouro Negro: peroxisome, endocytosis, and sulfur metabolism. Ouro Negro shows a less affected metabolism, which may lead to a more successful symbiosis. Proteins related to defense response were also induced in BRS Radiante, implying that their activation in this genotype may be partially responsible for its less efficient symbiosis. It is important to consider the difference in the life cycles of the two cultivars, as Ouro Negro had more time to adapt to the symbiosis than BRS Radiante. These results provide a basis for future studies to identify and explore biochemical mechanisms of nitrogen fixation in common beans beyond the roots. MenosThe common bean (Phaseolus vulgaris L.) can supply part of its N demand through biological nitrogen fixation (BNF) in symbiosis with rhizobia. However, the responses to inoculation and BNF's contribution to plant nutrition vary with the plant genotype. Here, we examined the proteome of two common bean genotypes (Ouro Negro and BRS Radiante) to determine shifts in leaf metabolism with two N sources: mineral N and inoculation with Rhizobium tropici CIAT899 (= SEMIA 4077 = BR 322). Data are available via ProteomeXchange with the identifier PXD034817. Through UPLC-MS/MS analysis, we iden- tified 917 proteins, 338 of which are differentially expressed. Here we focus on the 64 and 22 proteins differentially expressed in BRS Radiante and Ouro Negro, respectively, when comparing the proteome profiles of inoculated and N-fertilized plants. Different pathways in P. vulgaris were affected positively and negatively in BRS Radiante during symbiosis: glutathione metabolism, cysteine and methionine metabolism, phenylpropanoid biosynthesis, sulfur metabolism, peroxisome pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, carbon metabolism, and phagosome were the identified pathways. However, only three pathways were affected in Ouro Negro: peroxisome, endocytosis, and sulfur metabolism. Ouro Negro shows a less affected metabolism, which may lead to a more successful symbiosis. Proteins related to defense response were also induced in BRS Radiante, implying... Mostrar Tudo |
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Palavras-Chave: |
Protein expression; Symbiotic Nitrogen Fixation. |
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Thesagro: |
Phaseolus Vulgaris. |
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Thesaurus NAL: |
Mass spectrometry. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02761naa a2200265 a 4500
001 2153835
005 2023-05-18
008 2023 bl uuuu u00u1 u #d
022 $a0334-5114
024 7 $ahttps://doi.org/10.1007/s13199-023-00921-z$2DOI
100 1 $aSHIOSE, L.
245 $aProteomic analysis of common bean (Phaseolus vulgaris L.) leaves showed a more stable metabolism in a variety responsive to biological nitrogen fixation.$h[electronic resource]
260 $c2023
520 $aThe common bean (Phaseolus vulgaris L.) can supply part of its N demand through biological nitrogen fixation (BNF) in symbiosis with rhizobia. However, the responses to inoculation and BNF's contribution to plant nutrition vary with the plant genotype. Here, we examined the proteome of two common bean genotypes (Ouro Negro and BRS Radiante) to determine shifts in leaf metabolism with two N sources: mineral N and inoculation with Rhizobium tropici CIAT899 (= SEMIA 4077 = BR 322). Data are available via ProteomeXchange with the identifier PXD034817. Through UPLC-MS/MS analysis, we iden- tified 917 proteins, 338 of which are differentially expressed. Here we focus on the 64 and 22 proteins differentially expressed in BRS Radiante and Ouro Negro, respectively, when comparing the proteome profiles of inoculated and N-fertilized plants. Different pathways in P. vulgaris were affected positively and negatively in BRS Radiante during symbiosis: glutathione metabolism, cysteine and methionine metabolism, phenylpropanoid biosynthesis, sulfur metabolism, peroxisome pentose and glucuronate interconversions, amino sugar and nucleotide sugar metabolism, carbon metabolism, and phagosome were the identified pathways. However, only three pathways were affected in Ouro Negro: peroxisome, endocytosis, and sulfur metabolism. Ouro Negro shows a less affected metabolism, which may lead to a more successful symbiosis. Proteins related to defense response were also induced in BRS Radiante, implying that their activation in this genotype may be partially responsible for its less efficient symbiosis. It is important to consider the difference in the life cycles of the two cultivars, as Ouro Negro had more time to adapt to the symbiosis than BRS Radiante. These results provide a basis for future studies to identify and explore biochemical mechanisms of nitrogen fixation in common beans beyond the roots.
650 $aMass spectrometry
650 $aPhaseolus Vulgaris
653 $aProtein expression
653 $aSymbiotic Nitrogen Fixation
700 1 $aVIDAL, M. S.
700 1 $aHERINGER, A. S
700 1 $aVESPOLI, L. S.
700 1 $aSILVEIRA, V.
700 1 $aBALDANI, J. I.
700 1 $aJESUS, E. da C.
773 $tSymbiosis, Published: 10 May 2023.
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