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| Registros recuperados : 33 | |
| 23. |  | ALVES, W. C.; ROSSI, G. A. M.; LOPES, W. D. Z.; ALMEIDA, H. M. S.; MATHIAS, L. A.; VIDAL, A. M. C.; SOARES, V. E. Geospatial distribution and risk factors for bovine cysticercosis in the state of Rondônia, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 9, p. 931-936, setembro. 2017. Título em português: Distribuição espacial e fatores de risco para a cisticercose bovina no Estado de Rondônia, Brasil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 24. | | VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; SOUZA, A. da S.; LEDO, C. A. da S. Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers. Genetics and Molecular Research, v.14, n. 3, p. 7759-7770, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 25. | | VIDAL, Á. M.; SANTOS, K. C. F. dos; CARVALHO, M. de J. da S. de; CONCEIÇÃO, J. P. S.; SOUZA, A. da S.; MENEZES, M. C. Influência da posição de cultivo da microestaca na micropropagação da mandioca (Manihot esculenta Crantz). In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 28. | | SOUZA, K. A. de; SOUZA, A. da S.; MENEZES, M. C.; SANTOS, E. B.; SILVEIRA, D. M. de S.; VIDAL, Á. M. Efeito de concentrações de um fertilizante solúvel no cultivo in vitro da variedade de mandioca (Manihot Esculenta Crantz) batata (BGM 1660). In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 29. | | SANTOS, E. B.; SOUZA, A. da S.; SILVEIRA, D. M. de S.; MENDES, M. I. de S.; SOUZA, K. A. de; VIDAL, Á. M. Acúmulo de matérias fresca e seca em plantas de mandioca (Manihot esculenta Crantz) BGM 340 (CM-305/5) micropropagadas sob diferentes concentrações de um fertilizante solúvel. In: CONGRESSO BRASILEIRO DE MANDIOCA, 15., 2013, Salvador. Inovação e sustentabilidade: da raiz ao amido: trabalhos apresentados. Salvador: CBM: Embrapa, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 30. | | VIDAL, Á. M.; COSTA, M. A. P. de C.; SOUZA, A. da S.; ALMEIDA, W. A. B. de; SOUZA, F. V. D. In vitro regeneration and morphogenesis of somatic embryos of cassava. Revista Ciência Agronômica, v. 45, n. 3, p. 558-565, jul-set, 2014.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 31. | | VIEIRA, L. de J.; SANTANA, J. R. F. de; VIDAL, A. M.; ALVES, A. A. C.; SOUZA, A. da S.; LEDO, C. A. da S.; SOUZA, F. V. D. Use of multivariate analysis to evaluate the effect of sucrose on in vitro cassava conservation. African Journal of Biotechnology, February, v.14, n.5, p. 419-424, 2015.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 32. | | VIDAL, A. M. C.; SARAN NETTO, A. S.; VAZ, A. C. N.; CAPODIFÓGLIO, E.; GONÇALVES, A. C. S.; ROSSI, G. A. M.; FIGUEIREDO, A. S.; RUIZ, V. L. A. Pseudomonas spp.: contamination sources in bulk tanks of dairy farms. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 9, p. 941-948, setembro. 2017. Título em português: Pseudomonas spp.: fontes de contaminação em tanques de expansão em fazendas leiteiras.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 33. | | ROSSI, G. A. M.; ALMEIDA, H. M. S.; GUIMARÃES-PEIXOTO, R. P. M.; ACEVEDO-NIETO, E. C.; PINTO, P. S. A.; VIDAL, A. M. C.; MATHIAS, L. A.; OLIVEIRA, L. G. Testing pigs of non-technified rearing farms for seum antibodies against Taenia solium in a region of the state of São Paulo, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 3, p. 141-144, mar. 2016.| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 33 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
27/10/2015 |
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Data da última atualização: |
18/05/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; SOUZA, A. da S.; LEDO, C. A. da S. |
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Afiliação: |
Á. M. VIDAL, UFRB; L. J. VIEIRA; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF. |
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Título: |
Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v.14, n. 3, p. 7759-7770, 2015. |
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ISSN: |
1676-5680 |
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Idioma: |
Inglês |
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Conteúdo: |
Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system. MenosMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava p... Mostrar Tudo |
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Palavras-Chave: |
Marcado molecular. |
|
Thesagro: |
Anatomia vegetal; Banco de germoplasma; Mandioca; Meristema. |
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Thesaurus NAL: |
Cassava; Genetic markers; Germplasm. |
|
Categoria do assunto: |
G Melhoramento Genético |
|
Marc: |
LEADER 02450naa a2200289 a 4500
001 2027402
005 2023-05-18
008 2015 bl uuuu u00u1 u #d
022 $a1676-5680
100 1 $aVIDAL, Á. M.
245 $aGenetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers.$h[electronic resource]
260 $c2015
520 $aMolecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic complement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.
650 $aCassava
650 $aGenetic markers
650 $aGermplasm
650 $aAnatomia vegetal
650 $aBanco de germoplasma
650 $aMandioca
650 $aMeristema
653 $aMarcado molecular
700 1 $aVIEIRA, L. J.
700 1 $aFERREIRA, C. F.
700 1 $aSOUZA, F. V. D.
700 1 $aSOUZA, A. da S.
700 1 $aLEDO, C. A. da S.
773 $tGenetics and Molecular Research$gv.14, n. 3, p. 7759-7770, 2015.
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