Data/hora: 20/06/2024 21:43:42

Biblioteca(s): | Embrapa Mandioca e Fruticultura.
Data corrente: | 23/11/2010
Data da última atualização: | 25/11/2010
Autoria: | VIDAL, A. M.; SOUZA, F. V. D.; SOUZA, A. da S.; COSTA, M. A. P. C.
Afiliação: | Ádila Melo Vidal, UFRB; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; ANTONIO DA SILVA SOUZA, CNPMF; Maria Angélica Pereira de Carvalho Costa, UFRB.
Título: | Somatic embryogenesis in explants from cassava in vitro plants.
Ano de publicação: | 2010
Fonte/Imprenta: | In: INTERNATIONAL HORTICULTURAL CONGRESS, 28., 2010, Lisboa. Science and horticulture for people: programme & book of abstracts. Lisboa: ISHS, 2010. v. 1, p. 228. 1 CD-ROM.
Idioma: | Inglês
Notas: | T12.006, pdf.
Conteúdo: | Cassava is an important food for millions of people in Africa, Asia and Latin America. Despite its great importance, this crop still has some limiting factors regarding nutritional aspects, as well as its cultivation, because some varieties are quite affected by biotic and non biotic factors. To help to overcome those obstacles, several research groups are integrating an entire scientific knowledge to generate more productive cassava varieties and to improve the existing ones. As an alternative, Biotechnology is contributing with the use of different techniques, standing out the micropropagation for the production of healthy material for propagation, providing a high number of plants in a short period of time, and the somatic embryogenesis as a system of regeneration of plants, that is a basic requirement for the improvement of a selected variety through genetic transformation. The somatic embryogenesis can be an interesting in vitro plant regeneration system for cassava. The aim of this work was to study somatic embryogenesis and in vitro plant regeneration of Cigana Preta variety from shoot tip and young leaves from in vitro plants. The explants were cultivated in MS media with 8,0 and 12,0 mg·L-1 of 2,4-D or Picloram. Two media cultures were tested to the embryos development. Embryos in cotyledonal stage were inoculated in germination media constituted of vitamins and salts of MS, 2 ?M of cupper sulfate, 2,4 g·L-1 of Phytagel®, and 1,77 ?M of BAP. The major frequency of callus and number of embryos per explant was obtained with 8 mg·L-1 of auxin Picloram. The regenerated plants presented normal development and in a minimum period of four weeks were cultivated in specific media to Micropropagation showing a normal behavior in this media.
Thesagro: | Mandioca; Propagação Vegetativa; Variedade.
Thesaurus Nal: | cassava; Manihot; micropropagation; varieties.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Mandioca e Fruticultura (CNPMF)
Exemplares: | CNPMF - (27015-1) - UPC - PL - PP -  -  -  - PCT