Data/hora: 20/06/2024 12:58:56

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	23/12/2016	
Data da última atualização: 	07/02/2017	
Autoria: 	VIDAL, Á. M.; VIEIRA, L. J.; FERREIRA, C. F.; SOUZA, F. V. D.; LEDO, C. A. da S.	
Afiliação: 	Á. M. VIDAL, UFRB; L. J. VIEIRA, UFRB; CLAUDIA FORTES FERREIRA, CNPMF; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF.	
Título: 	Genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) evaluated using ISSR markers BRS Formosa: variedade de mandioca recomendada para uso industrial nas microrregiões de Valença, Jequié e Santo Antônio de Jesus (BA).	
Ano de publicação: 	2015	
Fonte/Imprenta: 	Genetics and Molecular Research, v.14, N.3,p. 7759-7770, 2015.	
Idioma: 	Português	
Conteúdo: 	Molecular markers are efficient for assessing the genetic fidelity of various species of plants after in vitro culture. In this study, we evaluated the genetic fidelity and variability of micropropagated cassava plants (Manihot esculenta Crantz) using inter-simple sequence repeat markers. Twenty-two cassava accessions from the Embrapa Cassava & Fruits Germplasm Bank were used. For each accession, DNA was extracted from a plant maintained in the field and from 3 plants grown in vitro. For DNA amplification, 27 inter-simple sequence repeat primers were used, of which 24 generated 175 bands; 100 of those bands were polymorphic and were used to study genetic variability among accessions of cassava plants maintained in the field. Based on the genetic distance matrix calculated using the arithmetic omplement of the Jaccard?s index, genotypes were clustered using the unweighted pair group method using arithmetic averages. The number of bands per primer was 2-13, with an average of 7.3. For most micropropagated accessions, the fidelity study showed no genetic variation between plants of the same accessions maintained in the field and those maintained in vitro, confirming the high genetic fidelity of the micropropagated plants. However, genetic variability was observed among different accessions grown in the field, and clustering based on the dissimilarity matrix revealed 7 groups. Inter-simple sequence repeat markers were efficient for detecting the genetic homogeneity of cassava plants derived from meristem culture, demonstrating the reliability of this propagation system.	
Thesagro: 	Mandioca.	
Thesaurus Nal: 	Cassava.	
Categoria do assunto: 	G Melhoramento Genético	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
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