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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
07/01/2019 |
Data da última atualização: |
18/12/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
MATTOS, V. da S.; MULET, K.; CARES, J. E.; GOMES, C. B.; FERNANDEZ, D.; SA, M. F. G. de; CARNEIRO, R. M. D. G.; CASTAGNONE-SERENO, P. |
Afiliação: |
VANESSA DA SILVA MATTOS, UNB; KARINE MULET, CNRS; JUVENIL ENRIQUE CARES, UNB; CESAR BAUER GOMES, CPACT; DIANA FERNANDEZ, IRD; MARIA FATIMA GROSSI DE SA, Cenargen; REGINA MARIA DECHECHI G CARNEIRO, Cenargen; PHILIPPE CASTAGNONE-SERENO, INRA. |
Título: |
Development of Diagnostic SCAR Markers for Meloidogyne graminicola, M. Oryzae, and M. salasi associated with irrigated rice fields in Americas. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Plant Disease, v. 103, p. 83-88, Jan. 2019. |
DOI: |
https://doi.org/10.1094/PDIS-12-17-2015-RE |
Idioma: |
Inglês Português |
Conteúdo: |
Root-knot nematodes (RKN) cause important production losses of rice (Oryza sativa L.) in the world. Together with Meloidogyne graminicola Golden and Birchfield 1965, M. oryzae Maas, Sanders and Dede, 1978 and M. salasi López, 1984 have been causing damages in irrigated rice fields in Central and South America. In addition, six other RKN species may occur in rice fields in other regions of the world. Correct identification of Meloidogyne spp. is difficult but essential for the management of rice RKNs. The objective of this study was to develop some species-specific molecular markers for the diagnosis of South American RKN rice-related species. Isozyme phenotypes indicated the occurrence of some RKN species in the Brazilian samples, namely M. graminicola, M. oryzae, M. javanica, and two cryptic species designated as Meloidogyne sp. 2 and Meloidogyne sp. 3. Random amplified polymorphic DNA (RAPD) analysis of 16 isolates revealed interspecific genetic polymorphism between Meloidogyne spp., but isolates belonging to the same species (i.e., sharing the same esterase phenotype) always clustered together, whatever the species considered. Specific SCAR markers of 230, 120, and 160 bp were developed for M. graminicola, M. oryzae, and M. salasi, respectively. These SCAR markers may be potential molecular tools for application in routine diagnostic procedures subject to their validation with other rice RKN field populations in the world. |
Palavras-Chave: |
Nematóide das galhas; South American; Species-specific molecular markers. |
Thesagro: |
Marcador Molecular; Nematóide; Oryza Sativa. |
Thesaurus Nal: |
Root-knot nematodes. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189842/1/Cesar-Bauer-pdis-12-17-2015-re.pdf
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Marc: |
LEADER 02419naa a2200301 a 4500 001 2103147 005 2019-12-18 008 2019 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1094/PDIS-12-17-2015-RE$2DOI 100 1 $aMATTOS, V. da S. 245 $aDevelopment of Diagnostic SCAR Markers for Meloidogyne graminicola, M. Oryzae, and M. salasi associated with irrigated rice fields in Americas.$h[electronic resource] 260 $c2019 520 $aRoot-knot nematodes (RKN) cause important production losses of rice (Oryza sativa L.) in the world. Together with Meloidogyne graminicola Golden and Birchfield 1965, M. oryzae Maas, Sanders and Dede, 1978 and M. salasi López, 1984 have been causing damages in irrigated rice fields in Central and South America. In addition, six other RKN species may occur in rice fields in other regions of the world. Correct identification of Meloidogyne spp. is difficult but essential for the management of rice RKNs. The objective of this study was to develop some species-specific molecular markers for the diagnosis of South American RKN rice-related species. Isozyme phenotypes indicated the occurrence of some RKN species in the Brazilian samples, namely M. graminicola, M. oryzae, M. javanica, and two cryptic species designated as Meloidogyne sp. 2 and Meloidogyne sp. 3. Random amplified polymorphic DNA (RAPD) analysis of 16 isolates revealed interspecific genetic polymorphism between Meloidogyne spp., but isolates belonging to the same species (i.e., sharing the same esterase phenotype) always clustered together, whatever the species considered. Specific SCAR markers of 230, 120, and 160 bp were developed for M. graminicola, M. oryzae, and M. salasi, respectively. These SCAR markers may be potential molecular tools for application in routine diagnostic procedures subject to their validation with other rice RKN field populations in the world. 650 $aRoot-knot nematodes 650 $aMarcador Molecular 650 $aNematóide 650 $aOryza Sativa 653 $aNematóide das galhas 653 $aSouth American 653 $aSpecies-specific molecular markers 700 1 $aMULET, K. 700 1 $aCARES, J. E. 700 1 $aGOMES, C. B. 700 1 $aFERNANDEZ, D. 700 1 $aSA, M. F. G. de 700 1 $aCARNEIRO, R. M. D. G. 700 1 $aCASTAGNONE-SERENO, P. 773 $tPlant Disease$gv. 103, p. 83-88, Jan. 2019.
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Registro original: |
Embrapa Clima Temperado (CPACT) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
15/01/2018 |
Data da última atualização: |
27/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
RODRIGUES, E. V.; DAHER, R. F.; SANTOS, A. dos; VIVAS, M.; MACHADO, J. C.; GRAVINA, G. D. A.; SOUZA, Y. P. de; VIDAL, A. K.; ROCHA, A. D. S.; FREITAS, R. S. |
Afiliação: |
E. V. Rodrigues, Embrapa Agroenergia; R. F. Daher, Universidade Estadual do Norte Fluminense; A. dos Santos, Universidade Estadual Norte Fluminense; M. Vivas, Universidade Estadual Norte Fluminense; JUAREZ CAMPOLINA MACHADO, CNPGL; G. D. A. Gravina, Universidade Estadual Norte Fluminense; Y. P. de Souza, Universidade Estadual Norte Fluminense; A. K. Vida, Universidade Federal Norte Fluminense; A. D. S. Rocha, Universidade Federal Norte Fluminense; R. S. Freitas, Universidade Federal Norte Fluminense. |
Título: |
Selecting elephant grass families and progenies to produce bioenergy through mixed models (REML/BLUP). |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 16, n. 2, 2017. |
Páginas: |
12 p. |
Idioma: |
Inglês |
Conteúdo: |
Abstract Brazil has great potential to produce bioenergy since it is located in a tropical region that receives high incidence of solar energy and presents favorable climatic conditions for such purpose. However, the use of bioenergy in the country is below its productivity potential. The aim of the current study was to select full-sib progenies and families of elephant grass (Pennisetum purpureum S.) to optimize phenotypes relevant to bioenergy production through mixed models (REML/BLUP). The circulating diallel-based crossing of ten elephant grass genotypes was performed. An experimental design using the randomized block methodology, with three repetitions, was set to assess both the hybrids and the parents. Each plot comprised 14-m rows, 1.40 m spacing between rows, and 1.40 m spacing between plants. The number of tillers, plant height, culm diameter, fresh biomass production, dry biomass rate, and the dry biomass production were assessed. Genetic-statistical analyses were performed through mixed models (REML/BLUP). The genetic variance in the assessed families was explained through additive genetic effects and dominance genetic effects; the dominance variance was prevalent. Families such as Capim Cana D'África x Guaçu/I.Z.2, Cameroon x Cuba-115, CPAC x Cuba-115, Cameroon x Guaçu/I.Z.2, and IAC-Campinas x CPAC showed the highest dry biomass production. The family derived from the crossing between Cana D'África and Guaçu/I.Z.2 showed the largest number of potential individuals for traits such as plant height, culm diameter, fresh biomass production, dry biomass production, and dry biomass rate. The individual 5 in the family Cana D'África x Guaçu/I.Z.2, planted in blocks 1 and 2, showed the highest dry biomass production. MenosAbstract Brazil has great potential to produce bioenergy since it is located in a tropical region that receives high incidence of solar energy and presents favorable climatic conditions for such purpose. However, the use of bioenergy in the country is below its productivity potential. The aim of the current study was to select full-sib progenies and families of elephant grass (Pennisetum purpureum S.) to optimize phenotypes relevant to bioenergy production through mixed models (REML/BLUP). The circulating diallel-based crossing of ten elephant grass genotypes was performed. An experimental design using the randomized block methodology, with three repetitions, was set to assess both the hybrids and the parents. Each plot comprised 14-m rows, 1.40 m spacing between rows, and 1.40 m spacing between plants. The number of tillers, plant height, culm diameter, fresh biomass production, dry biomass rate, and the dry biomass production were assessed. Genetic-statistical analyses were performed through mixed models (REML/BLUP). The genetic variance in the assessed families was explained through additive genetic effects and dominance genetic effects; the dominance variance was prevalent. Families such as Capim Cana D'África x Guaçu/I.Z.2, Cameroon x Cuba-115, CPAC x Cuba-115, Cameroon x Guaçu/I.Z.2, and IAC-Campinas x CPAC showed the highest dry biomass production. The family derived from the crossing between Cana D'África and Guaçu/I.Z.2 showed the largest number of potential individ... Mostrar Tudo |
Palavras-Chave: |
Pennisetum purpureum S. |
Thesaurus NAL: |
biomass production; plant breeding. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/171044/1/Cnpgl-2017-GMR-Selecting-elephant-grass-families.pdf
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Marc: |
LEADER 02565naa a2200277 a 4500 001 2085329 005 2023-01-27 008 2017 bl uuuu u00u1 u #d 100 1 $aRODRIGUES, E. V. 245 $aSelecting elephant grass families and progenies to produce bioenergy through mixed models (REML/BLUP).$h[electronic resource] 260 $c2017 300 $a12 p. 520 $aAbstract Brazil has great potential to produce bioenergy since it is located in a tropical region that receives high incidence of solar energy and presents favorable climatic conditions for such purpose. However, the use of bioenergy in the country is below its productivity potential. The aim of the current study was to select full-sib progenies and families of elephant grass (Pennisetum purpureum S.) to optimize phenotypes relevant to bioenergy production through mixed models (REML/BLUP). The circulating diallel-based crossing of ten elephant grass genotypes was performed. An experimental design using the randomized block methodology, with three repetitions, was set to assess both the hybrids and the parents. Each plot comprised 14-m rows, 1.40 m spacing between rows, and 1.40 m spacing between plants. The number of tillers, plant height, culm diameter, fresh biomass production, dry biomass rate, and the dry biomass production were assessed. Genetic-statistical analyses were performed through mixed models (REML/BLUP). The genetic variance in the assessed families was explained through additive genetic effects and dominance genetic effects; the dominance variance was prevalent. Families such as Capim Cana D'África x Guaçu/I.Z.2, Cameroon x Cuba-115, CPAC x Cuba-115, Cameroon x Guaçu/I.Z.2, and IAC-Campinas x CPAC showed the highest dry biomass production. The family derived from the crossing between Cana D'África and Guaçu/I.Z.2 showed the largest number of potential individuals for traits such as plant height, culm diameter, fresh biomass production, dry biomass production, and dry biomass rate. The individual 5 in the family Cana D'África x Guaçu/I.Z.2, planted in blocks 1 and 2, showed the highest dry biomass production. 650 $abiomass production 650 $aplant breeding 653 $aPennisetum purpureum S 700 1 $aDAHER, R. F. 700 1 $aSANTOS, A. dos 700 1 $aVIVAS, M. 700 1 $aMACHADO, J. C. 700 1 $aGRAVINA, G. D. A. 700 1 $aSOUZA, Y. P. de 700 1 $aVIDAL, A. K. 700 1 $aROCHA, A. D. S. 700 1 $aFREITAS, R. S. 773 $tGenetics and Molecular Research$gv. 16, n. 2, 2017.
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