Data/hora: 17/06/2024 11:20:42

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 12/09/2014
Data da última atualização: | 29/04/2024
Autoria: | MÜLLER, B. S. de F.; SAKAMOTO, T.; SILVEIRA, R. D. D.; ZAMBUSSI-CARVALHO, P. F.; PEREIRA, M.; PAPPAS JUNIOR, G. J.; COSTA, M. M. do C.; GUIMARÃES, C. M.; PEREIRA, W. J.; BRONDANI, C.; VIANELLO-BRONDANI, R. P.
Afiliação: | BÁRBARA SALOMÃO DE FARIA MÜLLER, BIOAGRO - UFV; TETSU SAKAMOTO, UFMG; RICARDO DIÓGENES DIAS SILVEIRA; PATRICIA FERNANDA ZAMBUSSI-CARVALHO, UFG; MARISTELA PEREIRA, UFG; GEORGIOS JOANIS PAPPAS JUNIOR, UNB; MARCOS MOTA DO CARMO COSTA, CENARGEN; CLEBER MORAIS GUIMARAES, CNPAF; WENDELL JACINTO PEREIRA; CLAUDIO BRONDANI, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Título: | Differentially expressed genes during flowering and grain filling in common bean (Phaseolus vulgaris) grown under drought stress conditions.
Ano de publicação: | 2014
Fonte/Imprenta: | Plant Molecular Biology Reporter, Athens, v. 32, p. 438-451, 2014.
DOI: | 10.1007/s11105-013-0651-7
Idioma: | Inglês
Conteúdo: | Drought stress, particularly during the flowering and grain-filling stages of growth, contributes to serious yield loss in common bean (Phaseolus vulgaris L.). The aim of this study was to identify genes induced in response to drought stress using transcriptome analysis of contrasting genotypes. Using leaf tissues of tolerant (BAT 477) and susceptible common bean genotypes (Pérola), collected at the flowering and grain-filling stages, four complementary deoxyribonucleic acid representational difference analysis subtractive libraries were constructed and then sequenced. A total of 7,203 (77.6 %) sequences with an average sequence size of 570 bp were considered valid, for a combined 4 Mbp sequence. According to a differential display analysis, 802 expressed sequence tags, distributed across 67 contigs, were differentially expressed by the tolerant (37 contigs) and susceptible genotypes (30 contigs) after identification under drought conditions during the two investigated plant developmental stages. Of these differential contigs, the 13 most frequent genes were exclusive to the tolerant genotype. Based on BLAST2GO, 73 % of the gene sequences were annotated and 12 % showed mapping results, with the highest similarity rate corresponding to Glycine max (41 %). According to gene ontology functional analysis, 48 % of the sequences were attributed to cell metabolic processes. Overall, 8.3 % of the transcribed sequences exhibited similarity to transcription factors, predominantly those of the AP2-EREBP family (97.8 %). Of the target sequences validated by quantitative real-time polymerase chain reaction, most genes showed an expression level that agreed with that predicted by in silico analysis. Thus, the drought transcriptome dataset is a valuable resource on the variation in these gene sequences, offering the opportunity to identify robust molecular markers tightly linked to trait-controlling loci for use in marker-assisted breeding.
Thesagro: | Deficiência hídrica; Feijão; Phaseolus vulgaris.
Thesaurus Nal: | Abiotic stress; Beans; Water stress.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/180004/1/Muller2014-Article-DifferentiallyExpressedGenesDu.pdf
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)

Exemplares:

CENARGEN - (35102-1) - UPC - AP - DD -  -  -  - DIS
CNPAF - (32985-1) - UPC - AP - DD - 2014.054 -  - 2014.054 - DIS