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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
11/12/2000 |
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Data da última atualização: |
17/08/2023 |
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Autoria: |
HOOKEY, J. V.; RICHARDSON, J. F.; COOKSON, B. D. |
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Título: |
Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Journal of Clinical Microbiology, v. 36, n. 4, p. 1083-1089, Apr. 1998. |
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DOI: |
10.1128/JCM.36.4.1083-1089.1998. |
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Idioma: |
Inglês |
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Conteúdo: |
A typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates. MenosA typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both stra... Mostrar Tudo |
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Palavras-Chave: |
Bacterial typing techniques; DNA bacterial; Methicillin Resistance. |
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Thesagro: |
Biologia Molecular; Microbiologia; Staphylococcus Aureus. |
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Thesaurus Nal: |
Coagulase positive staphylococci; Genotype; Polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02530naa a2200265 a 4500
001 1524190
005 2023-08-17
008 1998 bl uuuu u00u1 u #d
024 7 $a10.1128/JCM.36.4.1083-1089.1998.$2DOI
100 1 $aHOOKEY, J. V.
245 $aMolecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene.$h[electronic resource]
260 $c1998
520 $aA typing procedure for Staphylococcus aureus was developed based on improved PCR amplification of the coagulase gene and restriction fragment length polymorphism (RFLP) analysis of the product. All coagulase-positive staphylococci produced a single PCR amplification product of either 875, 660, 603, or 547 bp. Those strains of epidemic methicillin-resistant S. aureus 16 (EMRSA-16) studied all gave a product of 547 bp. PCR products were digested withAluI and CfoI, and the fragments were separated by gel electrophoresis. Ten distinct RFLP patterns were found among 85 isolates of methicillin-resistant S. aureus (MRSA) and 10 propagating strains (PS) of methicillin-sensitive S. aureus(MSSA) examined. RFLP patterns 1, 2, and 3 were specific to strains of EMRSA-3, -15, and -16, respectively. By contrast, RFLP patterns 4 and 5 were seen with a heterogeneous collection of strains, together with drug-resistant forms of S. aureus isolated in Europe and four propagating strains used for the international phage set. RFLP pattern 6 was given by the Airedale isolate and PS 95. RFLP pattern 7 encompassed EMRSA-2 (isolate 331), PS 94, and PS 96. An isolate from Germany gave RFLP pattern 8. Eight strains of MSSA gave patterns similar to those of methicillin-resistant strains (RFLP patterns 3, 4, 5, 6, and 7), but two, PS 42E and PS 71, gave unique RFLP patterns 9 and 10, respectively. The coagulase gene PCR products for 24 isolates of MRSA and two isolates of MSSA were sequenced for both strands. The sequences were aligned, and evolutionary lineages were inferred based on pairwise distances between isolates.
650 $aCoagulase positive staphylococci
650 $aGenotype
650 $aPolymerase chain reaction
650 $aBiologia Molecular
650 $aMicrobiologia
650 $aStaphylococcus Aureus
653 $aBacterial typing techniques
653 $aDNA bacterial
653 $aMethicillin Resistance
700 1 $aRICHARDSON, J. F.
700 1 $aCOOKSON, B. D.
773 $tJournal of Clinical Microbiology$gv. 36, n. 4, p. 1083-1089, Apr. 1998.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
03/11/2017 |
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Data da última atualização: |
13/04/2021 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
REIS, D. P.; VIANA, M. C. M.; SANTOS, K. K. B.; MELO, I. G.; OLIVEIRA-PAIVA, C. A.; GUIMARAES, L. J. M.; OLIVEIRA, M. C.; MARRIEL, I. E. |
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Afiliação: |
Universidade Federal de São João Del Rei; Epamig; Centro Universitário de Sete Lagoas - UNIFEMM; Universidade Federal de São João Del Rei; CHRISTIANE ABREU DE OLIVEIRA PAIVA, CNPMS; LAURO JOSE MOREIRA GUIMARAES, CNPMS; MAYCON CAMPOS OLIVEIRA, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS. |
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Título: |
Enzymatic activity in cerrado soil under crop-livestock-forest integration system in the central region of Minas Gerais. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 29.; ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 15., 2017, Foz do Iguaçu. Resumos... São Paulo: Sociedade Brasileira de Microbiologia, 2017. |
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Idioma: |
Inglês |
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Thesagro: |
Enzima; Nutriente. |
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Thesaurus NAL: |
Enzymes; Soil nutrient dynamics. |
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Categoria do assunto: |
S Ciências Biológicas |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166043/1/Enzymatic-activity-cerrado.pdf
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Marc: |
LEADER 00856nam a2200229 a 4500
001 2078804
005 2021-04-13
008 2017 bl uuuu u00u1 u #d
100 1 $aREIS, D. P.
245 $aEnzymatic activity in cerrado soil under crop-livestock-forest integration system in the central region of Minas Gerais.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 29.; ENCONTRO NACIONAL DE MICROBIOLOGIA AMBIENTAL, 15., 2017, Foz do Iguaçu. Resumos... São Paulo: Sociedade Brasileira de Microbiologia$c2017
650 $aEnzymes
650 $aSoil nutrient dynamics
650 $aEnzima
650 $aNutriente
700 1 $aVIANA, M. C. M.
700 1 $aSANTOS, K. K. B.
700 1 $aMELO, I. G.
700 1 $aOLIVEIRA-PAIVA, C. A.
700 1 $aGUIMARAES, L. J. M.
700 1 $aOLIVEIRA, M. C.
700 1 $aMARRIEL, I. E.
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Embrapa Milho e Sorgo (CNPMS) |
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