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| Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
Registro Completo |
Biblioteca(s): |
Embrapa Café. |
Data corrente: |
14/03/2011 |
Data da última atualização: |
14/03/2011 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ALMEIDA, J. D. de; BARROS, L. M. G.; SANTOS, D. B. M.; COTTA, M. G.; BARBOSA, E. A.; CAÇÃO, S. B.; EIRA, M. T. S. da; ALVES, G. S. C.; VINECKY, F.; PEREIRA, L. F. P.; SILVA, F. R. da; ANDRADE, A. C.; MARRACCINI, P.; CARNEIRO, M. |
Afiliação: |
JULIANA DANTAS DE ALMEIDA, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; IAPAR; MIRIAN THEREZINHA SOUZA DA EIRA, SAPC; LUIZ FILIPE PROTASIO PEREIRA, SAPC; ALAN CARVALHO ANDRADE, CENARGEN; CIRAD UMR-DAP; MAURO CARNEIRO, CENARGEN. |
Título: |
Prospection of tissue specific promoters in coffee. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil. |
Idioma: |
Inglês |
Conteúdo: |
The majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. MenosThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its resp... Mostrar Tudo |
Palavras-Chave: |
Coffee. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02510nam a2200277 a 4500 001 1880640 005 2011-03-14 008 2008 bl uuuu u00u1 u #d 100 1 $aALMEIDA, J. D. de 245 $aProspection of tissue specific promoters in coffee.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE ON COFFEE SCIENCE, 22. 2008, Campinas, São Paulo, Brazil.$c2008 520 $aThe majority of transgenic organisms reported in the literature have been made using constitutive promoters. However, there are economic, environmental and biosecurity related restrictions involving indiscriminate (constitutive) expression of heterologous genes. The usage of tissue-specific and induced promoters can resolve those issues by limiting the expression of a transgene to the necessary tissues and conditions. The promoters currently used at Embrapa are transnational properties, burdening the research and causing technological dependence. Therefore, the objective of this work was to find and characterize tissue and organ specific promoter in Coffea spp. We have used the Coffee genome database in silico tools to find genes preferentially expressed in root, leaf and fruit. In this way we found 72 organ-specific candidates: 18 apparently preferentially expressed on leaves, 14 on roots and 40 on fruits. Some of those candidates were tested in vitro using RT-PCR, semiquantitative PCR, northern blotting and qPCR assays. All four leaf-specific candidates tested (GCFo1, GCFo2, GCFo3 and GCFo4) and at least one of the two fruit-specific candidates tested (GCFr1 e GCFr2) where confirmed to be preferentially expressed on their respective organs. Temporal and spatial expression assays showed that GCFr2 has its expression peak at the endosperm, 180 days after flowering. The highest expressed genes of leaf (GCFo3 and GCFo4) and fruit (GCFr2) were used as probes to isolate its respective promoter through a BAC libraries screening or using the Genome Walker Universal Kit (Clontech). Results concerning gene expression and the molecular characterization of these genes will be presented. 653 $aCoffee 700 1 $aBARROS, L. M. G. 700 1 $aSANTOS, D. B. M. 700 1 $aCOTTA, M. G. 700 1 $aBARBOSA, E. A. 700 1 $aCAÇÃO, S. B. 700 1 $aEIRA, M. T. S. da 700 1 $aALVES, G. S. C. 700 1 $aVINECKY, F. 700 1 $aPEREIRA, L. F. P. 700 1 $aSILVA, F. R. da 700 1 $aANDRADE, A. C. 700 1 $aMARRACCINI, P. 700 1 $aCARNEIRO, M.
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Embrapa Café (CNPCa) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte. |
Data corrente: |
18/01/2012 |
Data da última atualização: |
05/03/2012 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
NASCIMENTO, R. K. do; KUNINARI-NASCIMENTO, R.; BLOCH JUNIOR, C.; VERBISCK, N. V. |
Afiliação: |
RENATA KUNINARI DO NASCIMENTO, Graduando em Química da UFMS; UFMS; CARLOS BLOCH JUNIOR, CENARGEN; NEWTON VALERIO VERBISCK, CNPGC. |
Título: |
Sequenciamento de novo de peptídeos por espectrometria de massas MALDI-TOF. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
In: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 7., 2011, CAMPO GRANDE, MS. [Anais da]... Campo Grande, MS: Embrapa Gado de Corte, 2011. |
Idioma: |
Português |
Conteúdo: |
Neste trabalho apresentam-se os resultados do estabelecimento dessa técnica no Laboratório de Proteômica e Espectrometria de Massas da Sanidade Animal/Embrapa Gado de Corte (PEMSA). No espectrômetro obtêm-se espectros com séries de massas, que por sua vez correspondem aos produtos da quebra ordenada de determinadas ligações presentes em cada molécula ionizada. |
Palavras-Chave: |
Aminoácidos; Macromoléculas biológicas; MALDI-TOF/TOF; Sequenciamento. |
Thesagro: |
Peptídeo; Proteína. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01127naa a2200229 a 4500 001 1912829 005 2012-03-05 008 2011 bl uuuu u00u1 u #d 100 1 $aNASCIMENTO, R. K. do 245 $aSequenciamento de novo de peptídeos por espectrometria de massas MALDI-TOF.$h[electronic resource] 260 $c2011 520 $aNeste trabalho apresentam-se os resultados do estabelecimento dessa técnica no Laboratório de Proteômica e Espectrometria de Massas da Sanidade Animal/Embrapa Gado de Corte (PEMSA). No espectrômetro obtêm-se espectros com séries de massas, que por sua vez correspondem aos produtos da quebra ordenada de determinadas ligações presentes em cada molécula ionizada. 650 $aPeptídeo 650 $aProteína 653 $aAminoácidos 653 $aMacromoléculas biológicas 653 $aMALDI-TOF/TOF 653 $aSequenciamento 700 1 $aKUNINARI-NASCIMENTO, R. 700 1 $aBLOCH JUNIOR, C. 700 1 $aVERBISCK, N. V. 773 $tIn: JORNADA CIENTÍFICA EMBRAPA GADO DE CORTE, 7., 2011, CAMPO GRANDE, MS. [Anais da]... Campo Grande, MS: Embrapa Gado de Corte, 2011.
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