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Registro Completo |
Biblioteca(s): |
Embrapa Amazônia Ocidental. |
Data corrente: |
29/05/2006 |
Data da última atualização: |
28/04/2022 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
SOUZA, I. C. de; SILVA, S. E. L. D. |
Afiliação: |
Pibic/CNPq; Embrapa Amazônia Ocidental.; SEBASTIAO EUDES LOPES DA SILVA, CPAA. |
Título: |
Efeito do nitrogênio, do fósforo e do potássio no desenvolvimento do porta-enxerto tangerina Cleópatra. |
Ano de publicação: |
2004 |
Fonte/Imprenta: |
In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 1., 2004, Manaus. Anais... Manaus: Embrapa Amazônia Ocidental, 2004. p. 80-85. (Embrapa Amazônia Ocidental. Documentos, 35). |
Idioma: |
Português |
Conteúdo: |
O objetivo deste trabalho foi avaliar o efeito do nitrogênio, do fósforo e do potássio no desenvolvimento do porta-enxerto tangerina Cleópatra. |
Palavras-Chave: |
Porta-enxerto. |
Thesagro: |
Fósforo; Nitrogênio; Potássio; Tangerina. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/82631/1/EfeitoNDoc-35.pdf
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Marc: |
LEADER 00832nam a2200181 a 4500 001 1678220 005 2022-04-28 008 2004 bl uuuu u00u1 u #d 100 1 $aSOUZA, I. C. de 245 $aEfeito do nitrogênio, do fósforo e do potássio no desenvolvimento do porta-enxerto tangerina Cleópatra. 260 $aIn: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AMAZÔNIA OCIDENTAL, 1., 2004, Manaus. Anais... Manaus: Embrapa Amazônia Ocidental, 2004. p. 80-85. (Embrapa Amazônia Ocidental. Documentos, 35).$c2004 520 $aO objetivo deste trabalho foi avaliar o efeito do nitrogênio, do fósforo e do potássio no desenvolvimento do porta-enxerto tangerina Cleópatra. 650 $aFósforo 650 $aNitrogênio 650 $aPotássio 650 $aTangerina 653 $aPorta-enxerto 700 1 $aSILVA, S. E. L. D.
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Registro original: |
Embrapa Amazônia Ocidental (CPAA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
12/12/2012 |
Data da última atualização: |
09/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
NASCIMENTO, C. S.; PEIXOTO, J. de O.; VERARDO, L. L.; CAMPOS, C. F.; WELLER, M. M. C.; FARIA, V. R.; BOTELHO, M. E.; MARTINS, M. F.; MACHADO, M. A.; SILVA, F. F.; LOPES, P. S.; GUIMARÃES, S. E. F. |
Afiliação: |
C. S. NASCIMENTO, UFV; JANE DE OLIVEIRA PEIXOTO, CNPSA; L. L. VERARDO, UFV; C. F. CAMPOS, UFV; M. M. C. WELLER, UFV; V. R. FARIA, UFV; M. E. BOTELHO, UFV; MARTA FONSECA MARTINS, CNPGL; MARCO ANTONIO MACHADO, CNPGL; F. F. SILVA, UFV; P. S. LOPES, UFV; S. E. F. GUIMARÃES, UFV. |
Título: |
Transcript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Genetics and Molecular Research, v. 11, n. 3, p. 3315-3328. 2012. |
DOI: |
https://doi.org/10.4238/2012.june.15.1 |
Idioma: |
Inglês |
Conteúdo: |
In general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds. MenosIn general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (... Mostrar Tudo |
Palavras-Chave: |
CDNA; Etiquetas de sequencia expressa. |
Thesagro: |
Músculo; Presunto. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/942276/1/Transcript-profiling-of-expressed-sequence-tag-from-semimembranosus-muscle.pdf
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Marc: |
LEADER 02646naa a2200313 a 4500 001 1942276 005 2024-02-09 008 2012 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.4238/2012.june.15.1$2DOI 100 1 $aNASCIMENTO, C. S. 245 $aTranscript profiling of expressed sequence tag from semimembranosus muscle of commercial and naturalized pig breeds.$h[electronic resource] 260 $c2012 520 $aIn general, genetic differences across different breeds of pig lead to variation in mature body size and slaughter age. The Commercial breeds Duroc and Large White and the local Brazilian breed Piau are ostensibly distinct in terms of growth and muscularity, commercial breeds are much leaner while local breeds grow much slower and are fat type pigs. However, the genetic factors that underlie such distinctions remain unclear. We used expressed sequence tags (ESTs) to characterize and compare transcript profiles in the semimembranosus muscle of these pig breeds. Our aim was to identify differences in breed-related gene expression that might influence growth performance and meat quality. We constructed three non-normalized cDNA libraries from semimembranosus muscle, using two samples from each one, of these three breeds; 6902 high-quality ESTs were obtained. Cluster analysis was performed and these sequences were clustered into 3670 unique sequences; 24.7% of the sequences were categorized as contigs and 75.3% of the sequences were singletons. Based on homology searches against the SwissProt protein database, we were able to assign a putative protein identity to only 1050 unique sequences. Among these, 58.5% were full-length protein sequences and 17.2% were pig-specific sequences. Muscle structural and cytoskeletal proteins, such as actin, and myosin, were the most abundant transcripts (16.7%) followed by those related to mitochondrial function (12.9%), and ribosomal proteins (12.4%). Furthermore, ESTs generated in this study provide a rich source for identification of novel genes and for the comparative analysis of gene expression patterns in divergent pig breeds. 650 $aMúsculo 650 $aPresunto 653 $aCDNA 653 $aEtiquetas de sequencia expressa 700 1 $aPEIXOTO, J. de O. 700 1 $aVERARDO, L. L. 700 1 $aCAMPOS, C. F. 700 1 $aWELLER, M. M. C. 700 1 $aFARIA, V. R. 700 1 $aBOTELHO, M. E. 700 1 $aMARTINS, M. F. 700 1 $aMACHADO, M. A. 700 1 $aSILVA, F. F. 700 1 $aLOPES, P. S. 700 1 $aGUIMARÃES, S. E. F. 773 $tGenetics and Molecular Research$gv. 11, n. 3, p. 3315-3328. 2012.
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