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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
28/11/1994 |
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Data da última atualização: |
28/11/1994 |
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Autoria: |
DAVIDE, L. C. |
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Título: |
Caracteristicas cromossomicas da madeira e das aciculas como uma contribuicao para a definicao taxonomica do Pinus de Tecunumani |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
Floresta, Curitiba, v.20, n.1/2, p.105-106, 1990 |
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ISSN: |
0015-3826 |
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Idioma: |
Português |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00409naa a2200121 a 4500
001 1283449
005 1994-11-28
008 1990 bl uuuu u00u1 u #d
022 $a0015-3826
100 1 $aDAVIDE, L. C.
245 $aCaracteristicas cromossomicas da madeira e das aciculas como uma contribuicao para a definicao taxonomica do Pinus de Tecunumani
260 $c1990
773 $tFloresta, Curitiba$gv.20, n.1/2, p.105-106, 1990
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Registro original: |
Embrapa Florestas (CNPF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
30/10/2020 |
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Data da última atualização: |
30/10/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VAZ, C. S. L.; RECH, D. V.; ALVES, L.; COLDEBELLA, A.; BRENTANO, L.; TREVISOL, I. M. |
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Afiliação: |
CLARISSA SILVEIRA LUIZ VAZ, CNPSA; DAIANE VOSS RECH, CNPSA; LUANA ALVES, UNC/Concórdia; ARLEI COLDEBELLA, CNPSA; LIANA BRENTANO, CNPSA; IARA MARIA TREVISOL, CNPSA. |
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Título: |
Effect of time of therapy with wild-type lytic bacteriophages on the reduction of Salmonella enteritidis in broiler chickens. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Veterinary Microbiology, v. 240, 2020. |
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DOI: |
https://doi.org/10.1016/j.vetmic.2019.108527 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Salmonella Enteritidis remains a leading cause of human foodborne disease, mostly associated with the consumption of contaminated poultry products. To more strategically implement a phage therapy scheme for S. Enteritidis control in broilers, a cocktail containing three wild-type lytic bacteriophages (LBs) previously isolated from chickens was evaluated shortly and later after a challenge. Genomic characterization, lytic spectrum and in vitro efficacy were determined for each studied LB. In independent trials, broilers challenged with S. Enteritidis on day of hatch received phage therapy from 6 to 10 days of age (early treatment), and from 31 to 35 days of age (later treatment). S. Enteritidis analyses were performed before treatment and at 1, 4, 7 and 10 days posttreatment (dpt) in both trials. Partial DNA sequence analysis of each LB revealed close similarity to the Ackermannviridae family. LBs lysed different Salmonella enterica serovars, while other tested bacteria were refractory. An in-vitro reduction of 1.49, 0.65 and 0.58 log10 CFU/mL in S. Enteritidis number was obtained after co-incubation for 3 h with each LB. Both in vivo trials showed a significant reduction in the average number of intestinal S. Enteritidis calculated after phage therapy compared with controls. However, the highest efficiency was found in the later therapy, which resulted in a reduction of 1.08 log10 CFU/g in the average from 4 to 10 dpt, showing potential for future use as a pre-harvest strategy to reduce the S. Enteritidis intestinal colonization in broilers on farms. MenosAbstract: Salmonella Enteritidis remains a leading cause of human foodborne disease, mostly associated with the consumption of contaminated poultry products. To more strategically implement a phage therapy scheme for S. Enteritidis control in broilers, a cocktail containing three wild-type lytic bacteriophages (LBs) previously isolated from chickens was evaluated shortly and later after a challenge. Genomic characterization, lytic spectrum and in vitro efficacy were determined for each studied LB. In independent trials, broilers challenged with S. Enteritidis on day of hatch received phage therapy from 6 to 10 days of age (early treatment), and from 31 to 35 days of age (later treatment). S. Enteritidis analyses were performed before treatment and at 1, 4, 7 and 10 days posttreatment (dpt) in both trials. Partial DNA sequence analysis of each LB revealed close similarity to the Ackermannviridae family. LBs lysed different Salmonella enterica serovars, while other tested bacteria were refractory. An in-vitro reduction of 1.49, 0.65 and 0.58 log10 CFU/mL in S. Enteritidis number was obtained after co-incubation for 3 h with each LB. Both in vivo trials showed a significant reduction in the average number of intestinal S. Enteritidis calculated after phage therapy compared with controls. However, the highest efficiency was found in the later therapy, which resulted in a reduction of 1.08 log10 CFU/g in the average from 4 to 10 dpt, showing potential for future use as a pre-harv... Mostrar Tudo |
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Palavras-Chave: |
Bacteriófagos. |
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Thesagro: |
Frango de Corte; Salmonella Enteritides. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02310naa a2200229 a 4500
001 2126163
005 2020-10-30
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetmic.2019.108527$2DOI
100 1 $aVAZ, C. S. L.
245 $aEffect of time of therapy with wild-type lytic bacteriophages on the reduction of Salmonella enteritidis in broiler chickens.$h[electronic resource]
260 $c2020
520 $aAbstract: Salmonella Enteritidis remains a leading cause of human foodborne disease, mostly associated with the consumption of contaminated poultry products. To more strategically implement a phage therapy scheme for S. Enteritidis control in broilers, a cocktail containing three wild-type lytic bacteriophages (LBs) previously isolated from chickens was evaluated shortly and later after a challenge. Genomic characterization, lytic spectrum and in vitro efficacy were determined for each studied LB. In independent trials, broilers challenged with S. Enteritidis on day of hatch received phage therapy from 6 to 10 days of age (early treatment), and from 31 to 35 days of age (later treatment). S. Enteritidis analyses were performed before treatment and at 1, 4, 7 and 10 days posttreatment (dpt) in both trials. Partial DNA sequence analysis of each LB revealed close similarity to the Ackermannviridae family. LBs lysed different Salmonella enterica serovars, while other tested bacteria were refractory. An in-vitro reduction of 1.49, 0.65 and 0.58 log10 CFU/mL in S. Enteritidis number was obtained after co-incubation for 3 h with each LB. Both in vivo trials showed a significant reduction in the average number of intestinal S. Enteritidis calculated after phage therapy compared with controls. However, the highest efficiency was found in the later therapy, which resulted in a reduction of 1.08 log10 CFU/g in the average from 4 to 10 dpt, showing potential for future use as a pre-harvest strategy to reduce the S. Enteritidis intestinal colonization in broilers on farms.
650 $aFrango de Corte
650 $aSalmonella Enteritides
653 $aBacteriófagos
700 1 $aRECH, D. V.
700 1 $aALVES, L.
700 1 $aCOLDEBELLA, A.
700 1 $aBRENTANO, L.
700 1 $aTREVISOL, I. M.
773 $tVeterinary Microbiology$gv. 240, 2020.
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