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Registro Completo |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros; Embrapa Unidades Centrais. |
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Data corrente: |
21/09/1994 |
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Data da última atualização: |
19/03/1998 |
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Autoria: |
PRASAD, M. V. R.; MAMEDE, F. B. F.; SILVA, F. P. da. |
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Título: |
Mutational improvement of peanut. |
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Ano de publicação: |
1985 |
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Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.20, n.4, p.439-445, abr. 1985. |
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Idioma: |
Inglês |
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Conteúdo: |
A study was initiated to improve the peanut variety Tatu (Arachis hypogaea L., ssp. fastigiata Waldron, var. fastigiata), through induction of mutations for increased pod number per plant and better pod filling, using a chemical mutagen sodium azide (NaN3). The treatment with sodium azide at a concentration of 3mM was found to be the most efficient as well as effective for induction of desirable mutants. Among the ten productive mutants selected with varying degress of improvement over the parent for the characters mentioned above, three of them Dwaf. Sd-HP and V3 consistently maintained their superiority in yield at significant levels over a period of three seasons including conditions of low rainfall. The mutants Dwarf and Sd-HP demostrated improvement in pod number per plant, seed number per pod, shelling percentage and harvest index in the M4 generation and superior yield levels. Use of these mutants in peanut breeding is discussed. |
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Palavras-Chave: |
Arachis hypogaea L; Chemical mutagem; Groundnut; Improvement; Melhoramento genético; Peanut; Peanut breeding. |
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Thesagro: |
Amendoim; Arachis Hypogaea; Mutação. |
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Thesaurus Nal: |
mutation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01676naa a2200277 a 4500
001 1104518
005 1998-03-19
008 1985 bl uuuu u00u1 u #d
100 1 $aPRASAD, M. V. R.
245 $aMutational improvement of peanut.
260 $c1985
520 $aA study was initiated to improve the peanut variety Tatu (Arachis hypogaea L., ssp. fastigiata Waldron, var. fastigiata), through induction of mutations for increased pod number per plant and better pod filling, using a chemical mutagen sodium azide (NaN3). The treatment with sodium azide at a concentration of 3mM was found to be the most efficient as well as effective for induction of desirable mutants. Among the ten productive mutants selected with varying degress of improvement over the parent for the characters mentioned above, three of them Dwaf. Sd-HP and V3 consistently maintained their superiority in yield at significant levels over a period of three seasons including conditions of low rainfall. The mutants Dwarf and Sd-HP demostrated improvement in pod number per plant, seed number per pod, shelling percentage and harvest index in the M4 generation and superior yield levels. Use of these mutants in peanut breeding is discussed.
650 $amutation
650 $aAmendoim
650 $aArachis Hypogaea
650 $aMutação
653 $aArachis hypogaea L
653 $aChemical mutagem
653 $aGroundnut
653 $aImprovement
653 $aMelhoramento genético
653 $aPeanut
653 $aPeanut breeding
700 1 $aMAMEDE, F. B. F.
700 1 $aSILVA, F. P. da
773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.20, n.4, p.439-445, abr. 1985.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
09/01/2019 |
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Data da última atualização: |
12/02/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
VASCONCELOS, M. J. V. de; ANTUNES, M. S.; OLIVEIRA, M. F. de; LOPES, M. A.; FIGUEIREDO, J. E. F. |
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Afiliação: |
MARIA JOSE VILACA DE VASCONCELOS, CNPMS; MAURICIO SCHUSTERSCHITZ ANTUNES, Colorado State University; MAURILIO FERNANDES DE OLIVEIRA, CNPMS; MAURICIO ANTONIO LOPES, DE/PR; JOSE EDSON FONTES FIGUEIREDO, CNPMS. |
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Título: |
Callus induction and plant regeneration from immature embryos culture of tropical maize. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 17, n. 3, p. 359-368, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
The development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli. MenosThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that ... Mostrar Tudo |
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Palavras-Chave: |
Calos tipo I e tipo II; Embriogênese somática. |
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Thesagro: |
Cultura de Tecido; Embrião Vegetal; Zea Mays. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/190064/1/Callus-induction.pdf
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Marc: |
LEADER 02500naa a2200229 a 4500
001 2103395
005 2019-02-12
008 2018 bl uuuu u00u1 u #d
100 1 $aVASCONCELOS, M. J. V. de
245 $aCallus induction and plant regeneration from immature embryos culture of tropical maize.$h[electronic resource]
260 $c2018
520 $aThe development of protocols to overcome the current limitations of callus induction and in vitro regeneration of highly recalcitrant tropical maize is crucial for plant genetic transformation. The ability of embryogenic callus (EC) formation of 46 tropical maize hybrids and 14 inbred lines was tested using N6 medium with the following changes: medium A (N6 + 15 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium B (N6 + 30 µM dicamba + 25 mM L-proline + 88 mM AgNO3), medium C (N6+ 30 µM dicamba + 6 mM L-proline), and medium D (N6+ 30 µM dicamba + 25 mM L-proline). Compact (Type I) and friable (Type II) callus were induced in the four media. Twenty genotypes produced callus in all media, and four genotypes (CO32, AG8012, CMS477BC4F2, and CMS-HGZ10) produced the highest number of callus (114, 134, 131, and 126 calli, respectively). All immature embryos of ten genotypes produced callus in at least one medium, while eight genotypes were highly recalcitrant and they did not produce any callus. The frequencies of EC ranged from 0% to 38%, and the highest rate of EC was observed on medium B (0.40) with a total of 865 calli, and the lowest induction rate was obtained with medium D (0.29) with 555 calli (P= 0.05). From the seventy-two EC of 26 genotypes transferred to Murashige & Skoog regeneration medium, twenty-four (66.7%) differentiated into green plants which produced seeds in R0 and R1 generations, and twelve (33.3%) developed into albino plants. The results demonstrated that the problem of the recalcitrant genotypes can be, at least partially, overcome by using immature embryos as explants together with tissue culture media formulations. Keywords: Zea mays, immature embryos, somatic embryogenesis, tissue culture, type I and type II calli.
650 $aCultura de Tecido
650 $aEmbrião Vegetal
650 $aZea Mays
653 $aCalos tipo I e tipo II
653 $aEmbriogênese somática
700 1 $aANTUNES, M. S.
700 1 $aOLIVEIRA, M. F. de
700 1 $aLOPES, M. A.
700 1 $aFIGUEIREDO, J. E. F.
773 $tRevista Brasileira de Milho e Sorgo, Sete Lagoas$gv. 17, n. 3, p. 359-368, 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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