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Registros recuperados : 32 | |
22. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ARAGÃO, K. G.; SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; TEIXEIRA, M.; VIEIRA, L. da S.; MONTEIRO, J. P. Caracterização da resistência a benzimidazóis e frequência de gêneros de nematóides gastrintestinais em um rebanho caprino da Embrapa Caprinos e Ovinos. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 6., 2017, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2017. p. 54-55. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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23. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MONTEIRO, J. P.; VASCONCELOS, J. F.; SANTOS, J. M. L. dos; FROTA, G. A.; TEIXEIRA, M.; BEVILAQUA, C. M. L.; VIEIRA, L. da S. Oxifendazole effect on gene expression of p-glycoproteins in two Haemonchus contortus isolates selected for resistance against ivermectin and oxifendazole. In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 27., 2019, Madison. Abstract book. Madison: WAAVP, 2019. p. 225. Abstract PS01.70 Biblioteca(s): Embrapa Caprinos e Ovinos. |
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24. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; FREITAS, E. P. de; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L.; MONTEIRO, J. P. Quantitative molecular diagnosis of levamisole resistance in populations of Haemonchus contortus. Experimental Parasitology, v. 205, article 107734, Aug. 2019. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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25. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | ARAUJO, P. R. M.; SANTOS, J. M. L. dos; VASCONCELOS, J. F.; VIEIRA, L. da S.; BEVILAQUA, C. M. L.; MONTEIRO, J. P. Polimorfismos relacionados à resistência a benzimidazóis em populações de Haemonchus contortus isoladas em rebanhos de ovinos no município de Bela Cruz, Ceará. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 5., 2016, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2016. p. 40-41. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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27. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | TEODORO, A. V.; PINTO ZEVALLOS, D. M.; MENESES, M. S.; ARRIGONI BLANK, M. de F.; OLIVEIRA, E. M. C.; SAMPAIO, T. S.; VASCONCELOS, J. F.; BLANK, A. F. Toxicity and repellency of the essential oil from Lippia gracilis to the coconut mite Aceria guerreronis (Acari: Eriophyidae). International Journal of Acarology, v. 47, n.5, p. 414-417, 2021. Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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28. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MONTEIRO, N. V.; SARAIVA, W. V. A.; DIAS, N. da S.; MACIEL, G. P. DE S.; VASCONCELOS, J. F.; DUARTE, P. M.; MELO, J. W. DA S.; RODRIGUES, S. M. M. Aspectos biológicos de Chrysoperla externa visando ao controle biológico da mosca-branca-do-cajueiro. Fortaleza: Embrapa Agroindústria Tropical, 2021. (Embrapa Agroindústria Tropical. Boletim de Pesquisa e Desenvolvimento, 215). Biblioteca(s): Embrapa Agroindústria Tropical. |
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29. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SARAIVA, W. V. A.; MONTEIRO, N. V.; DIAS, N. da S.; VASCONCELOS, J. F.; ALVES FILHO, E. de G.; MELO, J. W. da S.; MACEDO, V. H. M.; MACIEL, G. P. de S.; SOUSA NETO, E. P. de. Chrysoperla externa (Hagen) (Neuroptera, Chrysopidae) is a good candidate for predation of Aleurodicus cocois (Curtis) (Hemiptera: Aleyrodidae) in cashew crop. Arthropod-Plant Interactions, v. 17, n. 1, p. 99–110. Feb. 2023. Biblioteca(s): Embrapa Agroindústria Tropical. |
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30. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; RIBEIRO, W. L. C.; ANDDRÉ, W. P. P.; VIEIRA, L. da S.; TEIXEIRA, M.; BEVILAQUA, C. M. L.; MONTEIRO, J. P. Haemonchus contortus Beta-tubulin isotype 1 gene F200Y and F167Y SNPs are both selected by ivermectin and oxfendazole treatments with differing impacts on anthelmintic resistance. Veterinary Parasitology, v. 248, p. 90-95, Dec. 2017. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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31. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MONTEIRO, J. P.; SANTOS, J. M. L. dos; VASCONCELOS, J. F.; FROTA, G. A.; ANDRÉ, W. P. P.; RIBEIRO, W. L. C.; MELO, A. C. F. L.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. Molecular development of oxfendazole resistance starting with a susceptible Haemonchus contortus isolate. In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017.p. 516. Abstract 4588. In conjunction with 53rd MSPTM Annual Conference. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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32. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MONTEIRO, J. P.; SANTOS, J. M. L. dos; ANDRÉ, W. P. P.; VASCONCELOS, J. F.; FROTA, G. A.; RIBEIRO, W. L. C.; FREITAS, E. P. de; MACEDO, I. T. F.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. Molecular diagnostic for levamisol resistance in Haemonchus contortus. In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592. In conjunction with 53rd MSPTM Annual Conference. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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Registros recuperados : 32 | |
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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
09/11/2017 |
Data da última atualização: |
23/09/2019 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
MONTEIRO, J. P.; SANTOS, J. M. L. dos; ANDRÉ, W. P. P.; VASCONCELOS, J. F.; FROTA, G. A.; RIBEIRO, W. L. C.; FREITAS, E. P. de; MACEDO, I. T. F.; TEIXEIRA, M.; VIEIRA, L. da S.; BEVILAQUA, C. M. L. |
Afiliação: |
JOMAR PATRICIO MONTEIRO, CNPC; Jessica Maria Leite dos Santos, Pós-graduação - Universidade Estadual do Ceará (UECe) - Fortaleza, CE, Brazil; Weibson Paz Pinheiro Andre, UECe - Fortaleza, CE, Brazil; Janaelia Ferreira Vasconcelos, Universidade Estadual do Vale do Acaraú (UVA) - Sobral, CE, Brazil; Gracielle Araújo Frota, UVA - Sobral, CE, Brazil; Wesley Lyeverton Correia Ribeiro, UECe - Fortaleza, CE, Brazil; Edilson Pereira de Freitas; Iara Tersia Freitas Macedo; MARCEL TEIXEIRA, CNPC; LUIZ DA SILVA VIEIRA, CNPC; Claudia Maria Leal Bevilaqua, UECe - Fortaleza, CE, Brazil. |
Título: |
Molecular diagnostic for levamisol resistance in Haemonchus contortus. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592. |
Idioma: |
Inglês |
Notas: |
In conjunction with 53rd MSPTM Annual Conference. |
Conteúdo: |
Abstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast detection of levamisole resistance in H. contortus. MenosAbstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast ... Mostrar Tudo |
Palavras-Chave: |
Resistance to anthelmintics. |
Thesagro: |
Anti-Helmíntico; Caprino; Haemonchus Contortus; Ovino; Resistência a produtos químicos. |
Thesaurus NAL: |
Anthelmintics; Brazil; Drug resistance; Goats; Levamisole; Sheep; Small ruminants. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166470/1/cnpc-2017-Molecular-diagnostic.pdf
|
Marc: |
LEADER 02847nam a2200397 a 4500 001 2079274 005 2019-09-23 008 2017 bl uuuu u00u1 u #d 100 1 $aMONTEIRO, J. P. 245 $aMolecular diagnostic for levamisol resistance in Haemonchus contortus.$h[electronic resource] 260 $aIn: INTERNATIONAL CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENT OF VETERINARY PARASITOLOGY, 26., 2017, Kuala Lumpur, Malaysia. Abstract book. Perth: WAAVP, 2017. p. 381. Abstract 4592.$c4592 500 $aIn conjunction with 53rd MSPTM Annual Conference. 520 $aAbstract: In tropical areas, Haemonchus contortus is the leading cause of production losses in small ruminant herds and its control is traditionally done through the utilization of synthetic anthelmintics. Levamisole, an imidazothiazole derivative, is widely used in Brazil and the occurrence of resistance is not uncommon. The genetic base for levamisole resistance in H. contortus is still under investigation, but it has been recently associated with a 63 bp deletion in the Hco-acr-8 gene, which codes for one of the subunits of a levamisole-sensistive acetylcholine receptor. Here we describe a real time PCR test for the detection and quantification of the presence and absence of this deletion. Reactions contained 12.5ul 2 × Fast Start Universal SYBR Green Master Mix (Roche, West Sussex, UK), 0.3 pmol/ul of each primer (forward and reverse), 50 ng of DNA and water for a total volume of 25?l. Amplification conditions for were: 95 oC for 10 min and 35 cycles at 95 oC for 15 s and at 56 ºC for 30 s. We tested the assay in two known H. contortus isolates, one resistant (Kokstad isolate - KOK) and another susceptible (Inbred-susceptible-Edinburgh - ISE). We also used the test to characterize a local H. contortus population previously exposed to levamisole. Preliminay results are in agreement with previously reported data as only the resistant allele was detected in the KOK isolate and both alleles were detected in the ISE isolate suggesting that this test may be useful in the fast detection of levamisole resistance in H. contortus. 650 $aAnthelmintics 650 $aBrazil 650 $aDrug resistance 650 $aGoats 650 $aLevamisole 650 $aSheep 650 $aSmall ruminants 650 $aAnti-Helmíntico 650 $aCaprino 650 $aHaemonchus Contortus 650 $aOvino 650 $aResistência a produtos químicos 653 $aResistance to anthelmintics 700 1 $aSANTOS, J. M. L. dos 700 1 $aANDRÉ, W. P. P. 700 1 $aVASCONCELOS, J. F. 700 1 $aFROTA, G. A. 700 1 $aRIBEIRO, W. L. C. 700 1 $aFREITAS, E. P. de 700 1 $aMACEDO, I. T. F. 700 1 $aTEIXEIRA, M. 700 1 $aVIEIRA, L. da S. 700 1 $aBEVILAQUA, C. M. L.
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Embrapa Caprinos e Ovinos (CNPC) |
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