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| Registros recuperados : 222 | |
| 101. |  | POZZATTI, M.; GUERRA, C. C.; MARTINS, G.; SANTOS, I. D. dos; WAGNER, R.; FERRÃO, M. F.; MANFROI, V. Effects of winemaking on 'marselan' red wines: volatile compounds and sensory aspects. Ciência e Técnica Vitivinícola, v. 35, n. 2, p. 63-75, 2020.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 103. | | NICOLLI, K. P.; BIASOTO, A. C. T.; GUERRA, C. C.; SANTOS, H. P. dos; CORREA, L. C.; WELKE, J. E.; ZINI, A. C. Effects of Soil andVineyard Characteristics on Volatile, Phenolic Composition and Sensory Profile of Cabernet Sauvignon Wines of Campanha Gaúcha. Journal of the Brazilian Chemical Society, v. 00, n. 00, p. 1-15, 2019.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 104. | | NICOLLI, K. P.; BIASOTO, A. C. T.; GUERRA, C. C.; SANTOS, H. P. dos; CORREA, L. C.; WELKE, J. E.; ZINI, C. A. Effects of soil and vineyard characteristics on volatile, phenolic composition and sensory profile of cabernet sauvignon wines of Campanha Gaúcha. Journal Brazilian Chemical Society, v. 31, n. 6, p. 1110-1124, 2020.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 105. | | NICOLLI, K. P.; BIASOTO, A. C. T.; GUERRA, C. C.; SANTOS, H. P. dos; CORREA, L. C.; WELKE, J. E.; ZINI, C. A. Effects of soil and vineyard characteristics on volatile, phenolic composition and sensory profile of Cabernet Sauvignon wines of Campanha Gaúcha: supplementary information. Journal of the Brazilian Chemical Society, v. 31, 2020. Supplement.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 107. | | MANFROI, V.; COSTA, G. P.; GUERRA, C. C.; ZANUS, M. C.; FIALHO, F. B.; ROMBALDI, C. V. Aplicação de taninos enológicos na elaboração de vinho Cabernet Sauvignon e seus efeitos sobre a qualidade sensorial. Ciência Rural, Santa Maria, v. 40, n. 1, p. 175-181, 2010.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 109. | | PEREIRA, G. E.; SOARES, J. M.; GUERRA, C. C.; LIRA, M. M. P.; LIMA, M. D. de O.; SANTOS, J. de O. Caractérisation de vins rouges tropicaux produits au Nord-Est du Brésil. In: DEUTSCHER WEINBAUKONGRESS, 59.; INTERNATIONALES SYMPOSIUM INNOVATIONEN DER KELLERWIRTSCHAFT, 8., 2007, Stuttgart. p. 310.| Biblioteca(s): Embrapa Semiárido; Embrapa Uva e Vinho. |
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| 110. | | PEREIRA, G. E.; SOARES, J. M.; GUERRA, C. C.; LIRA, M. M. P.; LIMA, M. V. D. de O.; SANTOS, J. de O. Caractérisation de vins rouges tropicaux produits au Nord-Est du Brésil. In: DEUTSCHER WEINBAUKONGRESS, 59.; INTERNATIONALES SYMPOSIUM INNOVATIONEN DER KELLERWIRTSCHAFT, 8., 2007, Stuttgart. Np.| Biblioteca(s): Embrapa Semiárido. |
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| 111. | | MANFRÓI, V.; ZORZAN, C.; ZANUS, M. C.; GUERRA, C. C.; FIALHO, F. B.; AGNOL, I. D.; FERRI, V. C.; ROMBALDI, C. V. Capacidade antioxidante de vinhos tintos Cabernet Sauvignon tratados com taninos exógenos. In: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 11., 2007, Mendoza. Seduciendo al consumidor de hoy: anales. [Mendoza: INV: CLEIFRA], 2007. 1 CD-ROM. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 112. | | SANTOS, J. de O.; ALENCAR, Y. C. L. de; GUERRA, C. C.; LIRA, M. M. P.; PEREIRA, G. E. Avaliação do potencial enológico de uvas tintas colhidas em duas safras por ano no Vale do Sub-Médio São Francisco, Brasil. In: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 11., 2007, Mendoza. Seduciendo al consumidor de hoy: anales. [Mendoza: INV: CLEIFRA], 2007. 1 CD-ROM. Resumo.| Biblioteca(s): Embrapa Uva e Vinho. |
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| 113. | | SANTOS, J. de O.; ALENCAR, Y. C. L. de; GUERRA, C. C.; LIRA, M. M. P.; PEREIRA, G. E. Avaliação do potencial enológico de uvas tintas colhidas em duas safras por ano no Vale do Sub-Médio São Francisco, Brasil. In: CONGRESO LATINOAMERICANO DE VITICULTURA Y ENOLOGIA, 11., 2007, Mendonza. Seduciendo al concumidor de hoy. Mendonza: IVV: CLEIFRA: SECYT, 2007. 1 CD-ROM.| Biblioteca(s): Embrapa Semiárido. |
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| 118. | | OGLIARI, T. de C.; GUERRA, C. C.; CHALAÇA, M. Z.; GUGEL, G. M. Caracterização do perfil antociânico de vinhos tintos elaborados com diferentes fases sólidas na maceração. In: CONGRESSO LATINO-AMERICANO DE VITICULTURA E ENOLOGIA, 10.; CONGRESSO BRASILEIRO DE VITICULTURA E ENOLOGIA, 11.; SEMINÁRIO FRANCO-BRASILEIRO DE VITICULTURA E ENOLOGIA, 2., 2005, Bento Gonçalves. Anais... Bento Gonçalves: Embrapa Uva e Vinho, 2005. p. 319.| Biblioteca(s): Embrapa Uva e Vinho. |
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| Registros recuperados : 222 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
02/02/2023 |
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Data da última atualização: |
24/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
VANEGAS K. G.; RENDSVIG, J. K. H.; JARCZYSKA, Z. D.; CÔRTES, M. V. de C. B.; VAN ESCH, A. P.; MORERA-GÓMEZ, M.; CONTESINI, F. J.; MORTENSEN, U. H. |
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Afiliação: |
KATHERINA GARCIA VANEGAS, UNIVERSITY OF DENAMARK; JAKOB KRÆMMER HAAR RENDSVIG, UNIVERSITY OF DENMARK; ZOFIA DOROTA JARCZYNSKA, UNIVERSITY OF DENMARK; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; ABEL PETER VAN ESCH, UNIVERSITY OF DENAMARK; MARTÍ MORERA-GÓMEZ, UNIVERSITY OF DENAMARK; FABIANO JARES CONTESINI, UNIVERSITY OF DENAMARK; UFFE HASBRO MORTENSEN, UNIVERSITY OF DENAMARK. |
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Título: |
A Mad7 system for genetic engineering of filamentous fungi. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Journal of Fungi, v. 9, n. 1, 16, 2023. |
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ISSN: |
2309-608X |
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DOI: |
https://doi.org/10.3390/jof9010016 |
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Idioma: |
Inglês |
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Conteúdo: |
The introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories. |
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Palavras-Chave: |
CRISPR; Filamentous fungi; Fungal strain engineering; Mad7. |
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Thesagro: |
Fungo. |
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Thesaurus NAL: |
Aspergillus; Fungi; Genetic engineering. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151460/1/jof-2023.pdf
|
|
Marc: |
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022 $a2309-608X
024 7 $ahttps://doi.org/10.3390/jof9010016$2DOI
100 1 $aVANEGAS K. G.
245 $aA Mad7 system for genetic engineering of filamentous fungi.$h[electronic resource]
260 $c2023
520 $aThe introduction of CRISPR technologies has revolutionized strain engineering in filamentous fungi. However, its use in commercial applications has been hampered by concerns over intellectual property (IP) ownership, and there is a need for implementing Cas nucleases that are not limited by complex IP constraints. One promising candidate in this context is the Mad7 enzyme, and we here present a versatile Mad7-CRISPR vector-set that can be efficiently used for the genetic engineering of four different Aspergillus species: Aspergillus nidulans, A. niger, A. oryzae and A. campestris, the latter being a species that has never previously been genetically engineered. We successfully used Mad7 to introduce unspecific as well as specific template-directed mutations including gene disruptions, gene insertions and gene deletions. Moreover, we demonstrate that both single-stranded oligonucleotides and PCR fragments equipped with short and long targeting sequences can be used for efficient marker-free gene editing. Importantly, our CRISPR/Mad7 system was functional in both non-homologous end-joining (NHEJ) proficient and deficient strains. Therefore, the newly implemented CRISPR/Mad7 was efficient to promote gene deletions and integrations using different types of DNA repair in four different Aspergillus species, resulting in the expansion of CRISPR toolboxes in fungal cell factories.
650 $aAspergillus
650 $aFungi
650 $aGenetic engineering
650 $aFungo
653 $aCRISPR
653 $aFilamentous fungi
653 $aFungal strain engineering
653 $aMad7
700 1 $aRENDSVIG, J. K. H.
700 1 $aJARCZYSKA, Z. D.
700 1 $aCÔRTES, M. V. de C. B.
700 1 $aVAN ESCH, A. P.
700 1 $aMORERA-GÓMEZ, M.
700 1 $aCONTESINI, F. J.
700 1 $aMORTENSEN, U. H.
773 $tJournal of Fungi$gv. 9, n. 1, 16, 2023.
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Embrapa Arroz e Feijão (CNPAF) |
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