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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
19/07/2019 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PROENÇA, D. N.; SCHWAB, S.; VIDAL, M. S.; BALDANI, J. I.; XAVIER, G. R.; MORAIES, P. V. |
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Afiliação: |
University of Coimbra; STEFAN SCHWAB, CNPAB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB; GUSTAVO RIBEIRO XAVIER, CNPAB; University of Coimbra. |
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Título: |
The nematicide Serratia plymuthica M24T3 colonizes Arabidopsis thaliana, stimulates plant growth, and presents plant beneficial potential. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, Epub ahead of print, Published online, jun 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
Nine bacterial strains were previously isolated in association with pinewood nematode (PWN) from wilted pine trees. They proved to be nematicidal in vitro, and one of the highest activities, with potential to control PWN, was showed by Serratia sp. M24T3. Its ecology in association with plants remains unclear. This study aimed to evaluate the ability of strain M24T3 to colonize the internal tissues of the model plant Arabidopsis thaliana using confocal microscopy. Plant growth-promoting bacteria (PGPB) functional traits were tested and retrieved in the genome of strain M24T3. In greenhouse conditions, the bacterial effects of all nematicidal strains were also evaluated, co-inoculated or not with Bradyrhizobium sp. 3267, on Vigna unguiculata fitness. Inoculation of strain M24T3 increased the number of A. thaliana lateral roots and the confocal analysis confirmed effective bacterial colonization in the plant. Strain M24T3 showed cellulolytic activity, siderophores production, phosphate and zinc solubilization ability, and indole acetic acid production independent of supplementation with L-tryptophan. In the genome of strain M24T3, genes involved in the interaction with the plants such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinolytic activity, and quorum sensing were also detected. The genomic organization showed ACC deaminase and its leucine-responsive transcriptional regulator, and the activity of ACC deaminase was 594.6 nmol α-ketobutyrate μg protein-1 μl-1. Strain M24T3 in co-inoculation with Bradyrhizobium sp. 3267 promoted the growth of V. unguiculata. In conclusion, this study demonstrated the ability of strain M24T3 to colonize other plants besides pine trees as an endophyte and displays PGPB traits that probably increased plant tolerance to stresses. MenosNine bacterial strains were previously isolated in association with pinewood nematode (PWN) from wilted pine trees. They proved to be nematicidal in vitro, and one of the highest activities, with potential to control PWN, was showed by Serratia sp. M24T3. Its ecology in association with plants remains unclear. This study aimed to evaluate the ability of strain M24T3 to colonize the internal tissues of the model plant Arabidopsis thaliana using confocal microscopy. Plant growth-promoting bacteria (PGPB) functional traits were tested and retrieved in the genome of strain M24T3. In greenhouse conditions, the bacterial effects of all nematicidal strains were also evaluated, co-inoculated or not with Bradyrhizobium sp. 3267, on Vigna unguiculata fitness. Inoculation of strain M24T3 increased the number of A. thaliana lateral roots and the confocal analysis confirmed effective bacterial colonization in the plant. Strain M24T3 showed cellulolytic activity, siderophores production, phosphate and zinc solubilization ability, and indole acetic acid production independent of supplementation with L-tryptophan. In the genome of strain M24T3, genes involved in the interaction with the plants such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinolytic activity, and quorum sensing were also detected. The genomic organization showed ACC deaminase and its leucine-responsive transcriptional regulator, and the activity of ACC deaminase was 594.6 nmol α-ketobutyrate μg prot... Mostrar Tudo |
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Palavras-Chave: |
Deaminase; Endophytic colonization. |
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Thesaurus Nal: |
Arabidopsis thaliana; Genome; Nematode biology; Vigna unguiculata subsp. unguiculata. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02648naa a2200253 a 4500
001 2110760
005 2023-08-28
008 2019 bl uuuu u00u1 u #d
100 1 $aPROENÇA, D. N.
245 $aThe nematicide Serratia plymuthica M24T3 colonizes Arabidopsis thaliana, stimulates plant growth, and presents plant beneficial potential.$h[electronic resource]
260 $c2019
520 $aNine bacterial strains were previously isolated in association with pinewood nematode (PWN) from wilted pine trees. They proved to be nematicidal in vitro, and one of the highest activities, with potential to control PWN, was showed by Serratia sp. M24T3. Its ecology in association with plants remains unclear. This study aimed to evaluate the ability of strain M24T3 to colonize the internal tissues of the model plant Arabidopsis thaliana using confocal microscopy. Plant growth-promoting bacteria (PGPB) functional traits were tested and retrieved in the genome of strain M24T3. In greenhouse conditions, the bacterial effects of all nematicidal strains were also evaluated, co-inoculated or not with Bradyrhizobium sp. 3267, on Vigna unguiculata fitness. Inoculation of strain M24T3 increased the number of A. thaliana lateral roots and the confocal analysis confirmed effective bacterial colonization in the plant. Strain M24T3 showed cellulolytic activity, siderophores production, phosphate and zinc solubilization ability, and indole acetic acid production independent of supplementation with L-tryptophan. In the genome of strain M24T3, genes involved in the interaction with the plants such as 1-aminocyclopropane-1-carboxylate (ACC) deaminase, chitinolytic activity, and quorum sensing were also detected. The genomic organization showed ACC deaminase and its leucine-responsive transcriptional regulator, and the activity of ACC deaminase was 594.6 nmol α-ketobutyrate μg protein-1 μl-1. Strain M24T3 in co-inoculation with Bradyrhizobium sp. 3267 promoted the growth of V. unguiculata. In conclusion, this study demonstrated the ability of strain M24T3 to colonize other plants besides pine trees as an endophyte and displays PGPB traits that probably increased plant tolerance to stresses.
650 $aArabidopsis thaliana
650 $aGenome
650 $aNematode biology
650 $aVigna unguiculata subsp. unguiculata
653 $aDeaminase
653 $aEndophytic colonization
700 1 $aSCHWAB, S.
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
700 1 $aXAVIER, G. R.
700 1 $aMORAIES, P. V.
773 $tBrazilian Journal of Microbiology, Epub ahead of print, Published online, jun 2019
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 26 | |
| 8. |  | RIBEIRO, H. F. L; LAU, H. D.; SILVA, A. O. A.; SOUSA, J. S.; VALE, W. G. Preliminary report on artificial insemination in buffaloes of the Amazon region: through imported semen. In: WORLD BUFFALO CONGRESS, 4., 1994, São Paulo. Proceedings... [S.l.: s.n., 1994?]. v. 3, p. 591-593.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | SILVA, C. da; SOUZA, M. M. de; FILIMBERTI, E. L.; VALE, W. G. do; SILVA, A. F. da. Períodos de interferência de plantas daninhas na cultura do sorgo sacarino In: JORNADA CIENTÍFICA DA EMBRAPA AGROSSILVIPASTORIL, 2.,2013, Sinop. Anais... Sinop, MT: Embrapa Agrossilvipastoril, 2013. 1 CD.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| 11. | | SILVA, C.; SILVA, A. F. da; VALE, W. G. do; GALON, L.; PETTER, F. A.; MAY, A.; KARAM, D. Interferência de plantas daninhas na cultura do sorgo sacarino. Bragantia, Campinas, v. 73, n. 4, p. 1-8, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 12. | | RIBEIRO, H. F. L.; LAU, H. D.; SOUSA, J. S.; SILVA, A. O. A.; OHASHI, O. M.; VALE, W. G. Manual de inseminação artificial em bubalinos: alguns aspectos e recomendações básicas para a utilização vantajosa da inseminação artificial na espécie bubalina. Revista dos Criadores, v. 64, n. 770, p. 7-17, 1994. Caderno Especial.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 14. | | SHIRATSUCHI, L. S.; VALE, W. G. do; MALACARNE, T. J.; SCHUCK, C. M.; SILVA, R. G. da; OLIVEIRA JUNIOR, O. L. de. Algoritmos para aplicações de doses variáveis de nitrogênio em tempo real para produção de milho safra e safrinha no Cerrado. In: BERNARDI, A. C. de C.; NAIME, J. de M.; RESENDE, A. V. de; BASSOI, L. H.; INAMASU, R. Y. (Ed.). Agricultura de precisão: resultados de um novo olhar. Brasília, DF: Embrapa, 2014. cap. 17, p. 224-230.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Agrossilvipastoril; Embrapa Milho e Sorgo. |
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| 15. | | VALE, W. G.; MARQUES, J. R. F.; OHASHI, O. M.; SOUSA, J. S.; SILVA, A. O.; SOUZA, H. E. M. de; RIBEIRO, H. F. L. Buffalo reproduction and breeding in Brazil. In: SIPAR FALLOW-UP SEMINAR ON ANIMAL REPRODUCTION AND BIOTECHNOLOGY, 4., 1998, Belém, PA, Castanhal. Proceedings. [S.l.]: Swedish University of Agricultural Sciences/UFPA, [1998?]. v. 1, p. 130-150. il.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 16. | | CARDOSO, E. da C.; TEIXEIRA NETO, J. F.; SILVA, A. W. C. da; VEIGA, J. B. da; VALE, W. G.; ALENCAR, N. X. de. Deficiência de cálcio e fósforo em bubalinos no município de Portel, Estado do Pará. Belém, PA: EMBRAPA-CPATU, 1992. 20 p. il. (EMBRAPA-CPATU. Boletim de pesquisa, 135).| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 17. | | GARCIA, O. S.; VALE, W. G.; GARCIA, A. R.; RIBEIRO, H. F. L.; FERRO, R. S.; ROLIM FILHO, S. T.; SOUSA, E. M. Experimental study of testicular insulation in buffalo. Revista Veterinaria, v. 21, supl. 1, p. 889-891, 2010. Proceeding of World Buffalo Congress, 9., 2010, Buenos Aires.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 18. | | VALE, W. G.; RIBEIRO, H. F. L.; SOUZA, H. E. M.; SOUSA, J. S.; SILVA, A. O. A.; OHASHI, O. M. Factors affecting the efficiency of artificial insemination in buffaloes. In: WORLD BUFFALO CONGRESS, 5., 1997, Caserta, Italy. Proceedings. Caserta Italy: [s.n.], 1997. p. 812.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 19. | | VALE, W. G.; OHASHI, O. M.; SOUSA, J. S.; SILVA, A. O. A.; SOUZA, H. E. M. de; RIBEIRO, H. F. L. Factors affecting fertility of cattle and buffaloes in tropical environments. In: SIPAR FALLOW-UP SEMINAR ON ANIMAL REPRODUCTION AND BIOTECHNOLOGY, 4., 1998, Belém, PA, Castanhal. Proceedings. [S.l.]: Swedish University of Agricultural Sciences: UFPA, [1998?]. v. 1, p. 110-129.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 26 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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