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| Registros recuperados : 63 | |
| 4. |  | BRUMAT, A. E. L.; MOTTA, A. C. V.; MAEDA, S. Crescimento de Pinus taeda clonal influenciado pela fertilização e aplicação de resíduo de celulose. CONGRESSO FLORESTAL BRASILEIRO, 9., 2022, Brasília, DF. Inovações, desafios e oportunidades para o uso sustentável, a conservação das florestas brasileiras e o desenvolvimento social: anais. Brasília, DF: SBEF, 2022. p. 481-484.| Biblioteca(s): Embrapa Florestas. |
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| 7. | | BRUMAT, A. E. L.; MOTTA, A. C. V.; MAEDA, S. Diagnose nutricional em plantações clonais de P. taeda após fertilização mineral e aplicação de resíduo celulósico. CONGRESSO FLORESTAL BRASILEIRO, 9., 2022, Brasília, DF. Inovações, desafios e oportunidades para o uso sustentável, a conservação das florestas brasileiras e o desenvolvimento social: anais. Brasília, DF: SBEF, 2022. p. 199-202.| Biblioteca(s): Embrapa Florestas. |
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| 9. | | BARBOSA, J. Z.; ZAMBON, L. M.; MOTTA, A. C. V.; WENDLING, I. Composition, hot-water solubility of elements and nutritional value of fruits and leaves of yerba mate. Ciência e Agrotecnologia, Lavras, v. 39, n. 6, p. 593-603, Nov./Dec. 2015.| Biblioteca(s): Embrapa Florestas. |
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| 10. | | FAVARETTO, N.; MORAES, A. de; MOTTA, A. C. V.; PREVEDELLO, B. M. S. Efeito da revegetação e da adubação de area degradada na fertilidade do solo e nas características da palhada. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 2, p. 289-297, fev. 2000. Título em inglês: Effect of revegetation and of fertilization of degraded area on soil fertility and on characteristic of straw mulch.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 12. | | NICK, J. A.; YORINORI, G. T.; MOTTA, A. C. V.; SCOPEL, I.; FERNANDES, J. S. C. Efeitos de 11 anos de cultivo de café, em le, sobre parâmetros químicos do solo e crescimento de raíz, no municipio de Tomazina-PR. IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995. v.2, p. 633-635.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 20. | | BRUMAT, A. E. L.; MOTTA, A. C. V.; MAEDA, S.; GOMES, J. B. V. Crescimento inicial de plantio clonal de Pinus taeda após fertilização. Série Técnica IPEF, v. 26, n. 48, p. 574-579, 2023. Anais do Congresso de Plantações Florestais, 2023, Piracicaba.| Biblioteca(s): Embrapa Florestas. |
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| Registros recuperados : 63 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
27/07/2005 |
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Data da última atualização: |
11/05/2022 |
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Autoria: |
INGLIS, P. W.; RIGDEN, D. J.; MELLO, L. V.; LOUIS, E. J.; VALADARES-INGLIS, M. C. |
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Título: |
Monomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene. |
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Ano de publicação: |
2005 |
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Fonte/Imprenta: |
Molecular Genetics and Genomics, v. 274, p. 79-90, 2005. |
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Idioma: |
Inglês |
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Notas: |
Published online: 2 June 2005. |
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Conteúdo: |
In most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated. MenosIn most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses ... Mostrar Tudo |
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Palavras-Chave: |
Recombination; RecQ helicase; Telomere associated sequence. |
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Thesagro: |
Fungo; Metarhizium Anisopliae. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178387/1/ID-253071.pdf
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Marc: |
LEADER 02462naa a2200241 a 4500
001 1185898
005 2022-05-11
008 2005 bl uuuu u00u1 u #d
100 1 $aINGLIS, P. W.
245 $aMonomorphic subtelomeric DNA in the filamentous fungus, Metarhizium anisopliae, contains a RecQ helicase-like gene.$h[electronic resource]
260 $c2005
500 $aPublished online: 2 June 2005.
520 $aIn most filamentous fungi, telomere-associated sequences (TASs) are polymorphic, and the presence of restriction fragment length polymorphisms (RFLPs) may permit the number of chromosome ends to be estimated from the number of telomeric bands obtained by restriction digestion. Here, we describe strains of Metarhizium, Gliocladium and Paecilomyces species in which only one or a few telomeric bands of unequal intensity are detectable by Southern hybridization, indicating that interchromosomal TAS exchange occurs. We also studied an anomalous strain of Metarhizium anisopliae, which produces polymorphic telomeric bands larger than 8 kb upon digestion of genomic DNA with XhoI. In this case, the first XhoI site in from the chromosome end must lie beyond the presumed monomorphic region. Cloned telomeres from this strain comprise 18?26 TTAGGG repeats, followed at the internal end of the telomere tract by five repeats of the telomere-like sequence TAAACGCTGG. An 8.1-kb TAS clone also contains a gene for a RecQ-like helicase, designated TAH1, suggesting that this TAS is analogous to the Y elements in yeast and the subtelomeric helicase ORFs of Ustilago maydis (UTASRecQ) and Magnaporthe grisea (TLH1). The TAS in the anomalous strain of M. anisopliae, however, appears distinct from these in that it is found at most telomeres and its predicted protein product possesses a significantly longer N-terminal region in comparison to the M. grisea and U. maydis helicases. Hybridization analyses showed that TAH1 homologues are present in all other anomalous M. anisopliae strains studied, as well as in some other polymorphic strains, where the recQ-like gene also appears to be telomere-associated.
650 $aFungo
650 $aMetarhizium Anisopliae
653 $aRecombination
653 $aRecQ helicase
653 $aTelomere associated sequence
700 1 $aRIGDEN, D. J.
700 1 $aMELLO, L. V.
700 1 $aLOUIS, E. J.
700 1 $aVALADARES-INGLIS, M. C.
773 $tMolecular Genetics and Genomics$gv. 274, p. 79-90, 2005.
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