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70. | | URQUIAGA, S.; CRUZ, H. S.; BODDEY, R. M.; DOBEREINER, J. Fixação biologica de nitrogênio na cultura da cana-de-açúcar; estimativa da contribuição na cana-soca. In: , eds. In: SEMINARIO BIENAL DE PESQUISA DA UFRRJ, 4, MOSTRA COMUNITARIA DE PESQUISA, ENSINO E PRODUCAO, 1, Rio de Janeiro, 1988. Resumos... Rio de Janeiro, UFRRJ, Departamento de Pesquisa e Pos-Graduacao. p.62.. , 1988. Biblioteca(s): Embrapa Agrobiologia. |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Agrobiologia. |
Data corrente: |
20/04/1999 |
Data da última atualização: |
16/06/2008 |
Autoria: |
BODDEY, R. M.; SILVA, L. G. da; REIS, V.; ALVES, B. J. R.; URQUIAGA, S. |
Título: |
Assessment of bacterial nitrogen fixation in grass species. |
Ano de publicação: |
2000 |
Fonte/Imprenta: |
In: TRIPLETT, E. (Ed.). Prokayotic nitrogen fixation: a model system for analysis of a biological process. Wymondham: Horizon Scientific, 2000. |
Páginas: |
p. 705-726. |
Idioma: |
Inglês |
Conteúdo: |
A consensus now seems to be forming that the best strategy to develop a technology whereby cereal or grass crops will benefit significantly from plant associated biological nitrogen fixation (BNF) seems to lie in the improvement of existing plant/diazotroph associations. However, only a limited amount of research has been focussed on examining existing associations of N2-fixing micro-organisms with grasses or cereal for BNF inputs and ecological studies of the bacteria that found associated with field-grown plants. In this review we outline the techniques available to quantify BNF contributions to such associations, the problems associated with their application and discuss the existing evidence that indicates in which associations there are benefits of BNF which can be considered agronomically significant. We also discuss briefly the techniques available to enumerate populations of N2-fixing bacteria within plant tissues, giving an example from our studies using the ELISA (enzyme-linked immuno-absorbent assay) technique, the results of which showed very clearly that direct viable counts in semi-solid culture media seriously under-estimates actual bacterial numbers. |
Palavras-Chave: |
Biological nitrogen fixation; BNF; FBN; Fixação biológica de nitrogênio. |
Thesagro: |
Gramínea. |
Thesaurus NAL: |
Azospirillum; grasses. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01972naa a2200265 a 4500 001 1623848 005 2008-06-16 008 2000 bl --- 0-- u #d 100 1 $aBODDEY, R. M. 245 $aAssessment of bacterial nitrogen fixation in grass species. 260 $c2000 300 $ap. 705-726. 520 $aA consensus now seems to be forming that the best strategy to develop a technology whereby cereal or grass crops will benefit significantly from plant associated biological nitrogen fixation (BNF) seems to lie in the improvement of existing plant/diazotroph associations. However, only a limited amount of research has been focussed on examining existing associations of N2-fixing micro-organisms with grasses or cereal for BNF inputs and ecological studies of the bacteria that found associated with field-grown plants. In this review we outline the techniques available to quantify BNF contributions to such associations, the problems associated with their application and discuss the existing evidence that indicates in which associations there are benefits of BNF which can be considered agronomically significant. We also discuss briefly the techniques available to enumerate populations of N2-fixing bacteria within plant tissues, giving an example from our studies using the ELISA (enzyme-linked immuno-absorbent assay) technique, the results of which showed very clearly that direct viable counts in semi-solid culture media seriously under-estimates actual bacterial numbers. 650 $aAzospirillum 650 $agrasses 650 $aGramínea 653 $aBiological nitrogen fixation 653 $aBNF 653 $aFBN 653 $aFixação biológica de nitrogênio 700 1 $aSILVA, L. G. da 700 1 $aREIS, V. 700 1 $aALVES, B. J. R. 700 1 $aURQUIAGA, S. 773 $tIn: TRIPLETT, E. (Ed.). Prokayotic nitrogen fixation: a model system for analysis of a biological process. Wymondham: Horizon Scientific, 2000.
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