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Registros recuperados : 133 | |
81. | | SILVA, J. B. C. da; MELO, W. F. de; NUTTI, M. R. Batata-doce vitaminada. In: CONGRESSO PAN-AMERICANO DE INCENTIVO AO CONSUMO DE FRUTAS E HORTALIÇAS PARA A PROMOÇÃO DA SAÚDE, 5., 2009, Brasília, DF. Um prato cheio de saúde: anais... Brasília, DF: Ministério da Saúde, Coordenação-Geral da Política de Alimentação e Nutrição, 2009. CD-ROM. Biblioteca(s): Embrapa Hortaliças. |
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86. | | SILVA, C. S.; NICOLAU PINTO, M.; SILVA, J. B. C. da. Uniformidade e eficácia da desinsfestação de substratos utilizando vapor quente. Horticultura Brasileira, Brasília, v. 22, n. 2, jul. 2004. Suplemento 2. Trabalho apresentado no 44º Congresso Brasileiro de Olericultura, 2004. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 22, n. 2, p. 401, jul. 2004. Suplemento 1. Biblioteca(s): Embrapa Hortaliças. |
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89. | | CARVALHO, P. G. B. de; MACHADO, C. M. M.; VIEIRA, J. V.; SILVA, J. B. C. da. Influência da densidade de plantas no teor de carotenóides totais das cultivares de cenoura Nantes e Alvorada. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 389, jul. 2003. Suplemento 1. Biblioteca(s): Embrapa Hortaliças. |
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91. | | OLIVEIRA, J. A.; PEREIRA, C. E.; GUIMARÃES, R. M.; VIEIRA, A. R.; SILVA, J. B. C. da. Efeito de diferentes materiais de peletização na deterioração de sementes de tomate durante o armazenamento. Revista Brasileira de Sementes, Londrina, v. 25, n. 2, p. 20-27, dez. 2003. Biblioteca(s): Embrapa Hortaliças. |
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92. | | SILVA, E. D.; CUNHA, J. F.; FONSECA, M. E. N.; BOITEUX, L. S.; SILVA, J. B. C. da. Correlação entre conteúdo de carotenóides totais e colorimetria em acessos de batata-doce com raízes de diferentes colorações de polpa. Horticultura Brasileira, Brasília, DF, v. 25, n. 1, p. S95 ago. 2007. Suplemento 2. Suplemento. Resumo 511. Trabalho apresentado no 47. Congresso Brasileiro de Olericultura, 4. Simpósio Brasileiro sobre Cucurbitáceas, 2007. Porto Seguro. Biblioteca(s): Embrapa Hortaliças. |
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93. | | CUNHA, J. F. da; SILVA, E. D. da; FONSECA, M. E. N.; BOITEUX, L. S.; SILVA, J. B. C. da. Correlação da quantidade de carotenóides específicos de folhas e teor de betacaroteno em raizes de acessos de batata-doce com diferentes colorações de polpa. Horticultura Brasileira, Brasília, DF, v. 25, n. 1, p. S95, ago. 2007. Suplemento 2. Suplemento. Resumo 512. Trabalho apresentado no 47. Congresso Brasileiro de Olericultura, 4. Simpósio Brasileiro sobre Cucurbitáceas, 2007. Porto Seguro. Biblioteca(s): Embrapa Hortaliças. |
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94. | | CARVALHO, A. M.; JUNQUEIRA, A. M. R.; VIEIRA, J. R.; REIS, A.; SILVA, J. B. C. da; TOMITA, C. Severidade da queima das folhas e prevalência de patógenos associados à doença, em cenoura cultivada em sistema orgânico e convencional no verão do Distrito Federal. Fitopatologia Brasileira, Brasília, v. 28, p. 295, ago. 2003. Suplemento. Trabalho apresentado no 36º Congresso Brasileiro de Fitopatologia, 2003, Brasília. Resumo. Biblioteca(s): Embrapa Hortaliças. |
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96. | | OLIVEIRA, J. A.; PEREIRA, C. E.; GUIMARÃES, R. M.; VIEIRA, A. R.; SILVA, J. B. C. da. Desempenho de sementes de pimentão revestidas com diferentes materiais. Revista Brasileira de Sementes, Londrina, v. 25, n. 2, p. 36-47, dez. 2003. Biblioteca(s): Embrapa Hortaliças. |
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100. | | KIMOTO, T.; SILVA, J. B. C. da; SETUBAL, J. V.; REGHIN, M. Y.; LIMA, M. M. de A.; DUARTE, R. L. R. Efeito da eliminacao do escapo floral em diferentes estadios de crescimento sobre a produtividade de alho nobre. Horticultura Brasileira, Brasilia, v.13, n.1, p.88, maio 1995. Resumo. Biblioteca(s): Embrapa Hortaliças; Embrapa Meio-Norte. |
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Registros recuperados : 133 | |
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Registro Completo
Biblioteca(s): |
Embrapa Agricultura Digital. |
Data corrente: |
21/11/2012 |
Data da última atualização: |
14/04/2020 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 5 |
Autoria: |
D'AFONSECA, V.; SOARES, S. C.; ALI, A.; SANTOS, A. R.; PINTO, A. C.; MAGALHÃES, A. A. C.; FARIA, C. de J.; BARBOSA, E.; GUIMARÃES, L. C.; ESLABÃO, M.; ALMEIDA, S. S.; ABREU, V. A. C.; ZERLOTINI, A.; CARNEIRO, A. R.; CERDEIRA, L. T.; RAMOS, R. T. J.; HIRATA JÚNIOR, R.; MATTOS-GUARALDI, A. L.; TROST, E.; TAUCH, A.; SILVA, A.; SCHNEIDER, M. P.; MIYOSHI, A.; AZEVEDO, V. |
Afiliação: |
VÍVIAN D’AFONSECA, UFMG; SIOMAR C. SOARES, UFMG; AMJAD ALI, UFMG; ANDERSON R. SANTOS, UFMG; ANNE C. PINTO, UFMG; ARYANE A. C. MAGALHÃES, UFMG; CÁSSIO DE JESUS FARIA, UFMG; EUDES BARBOSA, UFMG; LUIS C. GUIMARÃES, UFMG; MARCUS ESLABÃO, UFPel; SINTIA S. ALMEIDA, UFMG; VINICIUS A. C. ABREU, UFMG; ADHEMAR ZERLOTINI NETO, CNPTIA; ADRIANA R. CARNEIRO, UFPA; LOUISE T. CERDEIRA, UFPA; ROMMEL T. J. RAMOS, UFPA; RAPHAEL HIRATA JÚNIOR, UERJ; ANA L. MATTOS-GUARALDI, UERJ; EVA TROST, Bielefeld University; ANDREAS TAUCH, Bielefeld University; ARTUR SILVA, UFPA; MARIA P. SCHNEIDER, UFPA; ANDERSON MIYOSHI, UFMG; VASCO AZEVEDO, UFMG. |
Título: |
Reannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Open Access Bioinformatics, v. 4, p. 1-13, 2012. |
Idioma: |
Inglês |
Conteúdo: |
Background: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl. MenosBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already des... Mostrar Tudo |
Palavras-Chave: |
Bioinformática. |
Thesagro: |
Genoma; Patogenicidade. |
Thesaurus NAL: |
Bioinformatics; Corynebacterium diphtheriae; Genome; Pathogenicity islands. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/70533/1/OAB-25500-re-annotation-of-the-corynebacterium-diphtheriae-nctc13129-g-022412.pdf
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Marc: |
LEADER 03832naa a2200481 a 4500 001 1940203 005 2020-04-14 008 2012 bl uuuu u00u1 u #d 100 1 $aD'AFONSECA, V. 245 $aReannotation of the Corynebacterium diphtheriae NCTC13129 genome as a new approach to studying gene targets connected to virulence and pathogenicity in diphtheria.$h[electronic resource] 260 $c2012 520 $aBackground: The reannotation of genomes already on file is a new approach to discovering new genetic elements and to make the genomes more descriptive and current with relevant features regarding the organism?s lifestyle. Within this approach, the present study aimed to reannotate the genome of the Gram-positive human pathogen Corynebacterium diphtheriae, which causes diphtheria. The deposit of massive amounts of information linked to other species of the genus Corynebacterium has facilitated the updating of the genomic interpretation of this microorganism. Additionally, the emergence of invasive disease by nontoxigenic strains of C. diphtheriae and the reemergence of diphtheria in partially immunized populations have given impetus to new studies in relation to its structural and functional genome. Results: In relation to structural genomics, 23 coding regions (coding sequences) were deleted and 71 new genes were added to the genome annotation. Nevertheless, all the pseudogenes were validated and ten new pseudogenes were created. In relation to functional genomics, about 57% of the genome annotation was updated and became functionally more informative. The product descriptions of 41% (973 proteins) were updated. Among them, 370 that were previously annotated as ?hypothetical proteins,? now have more informative descriptions. With the new annotation, the plasticity of the genome became evident, which shows improvements in the annotation of 13 pathogenicity islands already described in the literature. In addition, the large number of transposases and the presence of structural genes of bacteriophages make their genomic versatility evident. Contrasting with this reality, it also allowed the clarification of some aspects concerned with mechanisms used by C. diphtheriae to stop the invasion of the genome by bacteriophages, mediated by the clustered regularly interspaced short palindromic repeats region. Conclusion: The reannotation of the C. diphtheriae genome provided an improvement in annotation of the C. diphtheriae genome in several aspects, such as virulence characteristics and plasticity events. Moreover, the protocol used here can be extended to various other pathogens in order to improve the genomic information already on file in public databases and to minimize propagating errors. The reannotated archive and updated archive are available at: http://lgcm.icb.ufmg.br/pub/C_diphtheriae_reannotation.embl. 650 $aBioinformatics 650 $aCorynebacterium diphtheriae 650 $aGenome 650 $aPathogenicity islands 650 $aGenoma 650 $aPatogenicidade 653 $aBioinformática 700 1 $aSOARES, S. C. 700 1 $aALI, A. 700 1 $aSANTOS, A. R. 700 1 $aPINTO, A. C. 700 1 $aMAGALHÃES, A. A. C. 700 1 $aFARIA, C. de J. 700 1 $aBARBOSA, E. 700 1 $aGUIMARÃES, L. C. 700 1 $aESLABÃO, M. 700 1 $aALMEIDA, S. S. 700 1 $aABREU, V. A. C. 700 1 $aZERLOTINI, A. 700 1 $aCARNEIRO, A. R. 700 1 $aCERDEIRA, L. T. 700 1 $aRAMOS, R. T. J. 700 1 $aHIRATA JÚNIOR, R. 700 1 $aMATTOS-GUARALDI, A. L. 700 1 $aTROST, E. 700 1 $aTAUCH, A. 700 1 $aSILVA, A. 700 1 $aSCHNEIDER, M. P. 700 1 $aMIYOSHI, A. 700 1 $aAZEVEDO, V. 773 $tOpen Access Bioinformatics$gv. 4, p. 1-13, 2012.
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