Data/hora: 16/06/2024 19:16:33

Biblioteca(s): | Embrapa Semiárido.
Data corrente: | 21/06/2017
Data da última atualização: | 18/01/2018
Autoria: | SILVA, L. B.; TORRES, E. B.; NOBREGA, R. A. S.; LOPES, G. N.; VOGADO, R. F.; PAVAN, B. E.; FERNANDES JUNIOR, P. I.
Afiliação: | L. B. SILVA, Graduate Program in Agronomy-Crop Science, Federal University of Piaui; E. B. TORRES, Graduate Program in Agronomy-Crop Science, Federal University of Piaui; R. A. S. NÓBREGA, Federal University of Recôncavo da Bahia (UFRB), Center for Agrarian,Environmental and Biological Sciences; G. N. LOPES, Graduate Program in Agronomy-Crop Science, Federal University of Piaui; R. F. VOGADO, Graduate Program in Agronomy-Crop Science, Federal University of Piaui; B. E. PAVAN, Departament of Plant Science Socio-Economics and Food Technology of Ilha Solteira UNESP-FEIS Faculty of Engineering,State University of São Paulo; PAULO IVAN FERNANDES JUNIOR, CPATSA.
Título: | Biochemical studies of amylase, lipase and protease in Callosobruchus maculatus (Coleoptera: Chrysomelidae) populations fed with Vigna unguiculata grain cultivated with diazotrophic bacteria strains.
Ano de publicação: | 2017
Fonte/Imprenta: | Bulletin of Entomological Research, v. 107, p. 820-827, 2017.
DOI: | 10.1017/S0007485317000463
Idioma: | Inglês
Conteúdo: | The objective of this study was to evaluate the enzymatic activity of homogenates of insects fed on grain of cowpea, Vigna unguiculata (L.), cultivars grown with different nitrogen sources. For the experiment we used aliquots of the homogenate of 100 unsexed adult insects, emerged from 10 g of grain obtained from four cowpea cultivars: 'BRS Acauã', 'BRS Carijó', 'BRS Pujante', and 'BRS Tapaihum' grown under different regimes of nitrogen sources: mineral fertilizer, inoculation with strains of diazotrophs (BR 3267, BR 3262, BR 3299; INPA 03-11B, 03-84 UFLA, as well as the control (with soil nitrogen). The parameters evaluated were enzymatic activities of insect protease, amylase and lipase and the starch content of the grains. There were differences in the enzymatic activity of amylase, lipase and protease of insect homogenate according to the food source. A lower activity of the enzyme amylase from C. maculatus homogenate was observed when insects were fed grain of the cultivar BRS Carijó. A lower activity of lipase enzyme from C. maculatus homogenate was observed when the insects fed on grain from the interaction of the cultivar Tapaihum inoculated with BR 3262 diazotrophs. The lowest proteolytic activity was observed in homogenate of insects fed on interaction of 'BRS Carijó' inoculated with BR 3262 diazotrophs. Starch content correlated positively with the amylase activity of C. maculatus homogenate. The cultivar BRS Carijó had a different behavior from the other cultivars, according to the cluster analysis.
Palavras-Chave: | Adubação nitrogenada; Cowpea; Cowpea weevil; Digestive enzyme; Feijão caupi; Nitrogen fertilization.
Thesagro: | Feijão de corda; Fixação de nitrogênio; Inoculação; Vigna Unguiculata.
Thesaurus Nal: | Carbohydrate metabolism; Nitrogen fixation.
Categoria do assunto: | A Sistemas de Cultivo
Registro original: | Embrapa Semiárido (CPATSA)
Exemplares: | CPATSA - (56638-1) - UPC - AP - DD -  -  -  - DIS