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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
17/06/2008 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BOMFETI, C. A.; SOUZA-PACCOLA, E. A.; MASSOLA JUNIOR, N. S.; MARRIEL, I. E.; MEIRELLES, W. F.; CASELA, C. R.; PACCOLA-MEIRELLES, L. D. |
Afiliação: |
Cleide A. Bomfeti, UEL; Edneia A. Sopuza-Paccola, UEL; Nelson S. Massola Junior, Esalq; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; Carlos Roberto Casela, CNPMS; Luzia D. Paccola-Meirelles, UEL. |
Título: |
Localization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques. |
Ano de publicação: |
2008 |
Fonte/Imprenta: |
Tropical Plant Pathology, Brasília, v. 33, n. 1, p. 63-66, Jan./Feb. 2008. |
DOI: |
10.1590/S1982-56762008000100010 |
Idioma: |
Inglês |
Conteúdo: |
The etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. MenosThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. T... Mostrar Tudo |
Palavras-Chave: |
Doença bacteriana do milho; doenças foliares do milho; Maize bacterial disease; Maize leaf disease. |
Thesagro: |
Milho; Zea Mays. |
Categoria do assunto: |
H Saúde e Patologia |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32277/1/Localization-pantoea.pdf
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Marc: |
LEADER 02604naa a2200277 a 4500 001 1491172 005 2018-05-24 008 2008 bl uuuu u00u1 u #d 024 7 $a10.1590/S1982-56762008000100010$2DOI 100 1 $aBOMFETI, C. A. 245 $aLocalization of Pantoea ananatis inside lesions of maize white spot disease using transmission electron microscopy and molecular techniques.$h[electronic resource] 260 $c2008 520 $aThe etiological agent of maize white spot (MWS) disease has been a subject of controversy and discussion. Initially the disease was described as Phaeosphaeria leaf spot caused by Phaeosphaeria maydis. Other authors have suggested the existence of different fungal species causing similar symptoms. Recently, a bacterium, Pantoea ananatis, was described as the causal agent of this disease. The purpose of this study was to offer additional information on the correct etiology of this disease by providing visual evidence of the presence of the bacterium in the interior of the MWS lesions by using transmission electron microscopy (TEM) and molecular techniques. The TEM allowed visualization of a large amount of bacteria in the intercellular spaces of lesions collected from both artificially and naturally infected plants. Fungal structures were not visualized in young lesions. Bacterial primers for the 16S rRNA and rpoB genes were used in PCR reactions 10 amplify DNA extracted from water-soaked (young) and necrotic lesions. The universal fungal oligonucleotide ITS4 was also included to identify the possible presence of funga! structures inside lesions. Positive PCR products from water-soaked lesions, both from naturally and artificially inoculated plants, were produced with bacterial primers, whereas no amplification was observed when ITS4 oligonucleotide was used. On the other hand, DNA amplification with ITS4 primer was observed when DNA was isolated from necrotic (old) lesions. These results reinforced previous report of P. ananatis as the primary pathogen and the hypothesis that fungal species may colonize lesions pre-established by P. ananatis. 650 $aMilho 650 $aZea Mays 653 $aDoença bacteriana do milho 653 $adoenças foliares do milho 653 $aMaize bacterial disease 653 $aMaize leaf disease 700 1 $aSOUZA-PACCOLA, E. A. 700 1 $aMASSOLA JUNIOR, N. S. 700 1 $aMARRIEL, I. E. 700 1 $aMEIRELLES, W. F. 700 1 $aCASELA, C. R. 700 1 $aPACCOLA-MEIRELLES, L. D. 773 $tTropical Plant Pathology, Brasília$gv. 33, n. 1, p. 63-66, Jan./Feb. 2008.
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Embrapa Milho e Sorgo (CNPMS) |
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Biblioteca(s): |
Embrapa Clima Temperado; Embrapa Hortaliças. |
Data corrente: |
02/08/2022 |
Data da última atualização: |
11/08/2022 |
Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
Circulação/Nível: |
B - 1 |
Autoria: |
SILVA, G. O. da; AZEVEDO, F. Q.; MELO, J. W. P. de; PEREIRA, G. E.; PATIÑO-TORRES, A. J.; CARVALHO, A. D. F. de; RAGASSI, C. F.; PEREIRA, A. da S. |
Afiliação: |
GIOVANI OLEGARIO DA SILVA, CNPH; FERNANDA QUINTANILHA AZEVEDO, CPACT; JACKSON W. P. DE MELO, UNB; GABRIEL E. PEREIRA, UNB; ALBANIA J. PATIÑO-TORRES, Universidade de São Paulo; AGNALDO D FERREIRA DE CARVALHO, CNPH; CARLOS FRANCISCO RAGASSI, CNPH; ARIONE DA SILVA PEREIRA, CPACT. |
Título: |
Growth, fresh mass accumulation and distribution in new Brazilian potato cultivars. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Horticultura Brasileira, v. 40, n. 2, p. 208-213, Apr./June 2022. |
DOI: |
https://doi.org/10.1590/s0102-0536-20220210 |
Idioma: |
Inglês |
Conteúdo: |
O presente estudo objetivou avaliar a curva de crescimento das plantas de duas novas cultivares de batata, para melhor conhecer a dinâmica do desenvolvimento das plantas ao longo do ciclo de crescimento vegetativo, e para subsidiar eventuais estratégias diferenciadas de manejo. |
Palavras-Chave: |
Cultivar BRS F183 Potira; Cultivar BRS F50 Cecília. |
Thesagro: |
Batata; Crescimento. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145386/1/Artigo-BRS-F183.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1145126/1/Artigo-BRS-F183.pdf
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Marc: |
LEADER 01113naa a2200265 a 4500 001 2145386 005 2022-08-11 008 2022 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1590/s0102-0536-20220210$2DOI 100 1 $aSILVA, G. O. da 245 $aGrowth, fresh mass accumulation and distribution in new Brazilian potato cultivars.$h[electronic resource] 260 $c2022 520 $aO presente estudo objetivou avaliar a curva de crescimento das plantas de duas novas cultivares de batata, para melhor conhecer a dinâmica do desenvolvimento das plantas ao longo do ciclo de crescimento vegetativo, e para subsidiar eventuais estratégias diferenciadas de manejo. 650 $aBatata 650 $aCrescimento 653 $aCultivar BRS F183 Potira 653 $aCultivar BRS F50 Cecília 700 1 $aAZEVEDO, F. Q. 700 1 $aMELO, J. W. P. de 700 1 $aPEREIRA, G. E. 700 1 $aPATIÑO-TORRES, A. J. 700 1 $aCARVALHO, A. D. F. de 700 1 $aRAGASSI, C. F. 700 1 $aPEREIRA, A. da S. 773 $tHorticultura Brasileira$gv. 40, n. 2, p. 208-213, Apr./June 2022.
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Embrapa Hortaliças (CNPH) |
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