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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
30/09/2009 |
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Data da última atualização: |
10/08/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CHAVES, G. de M.; LISERRE, A. M.; ZACARHENCO, P. B.; SANTOS, K. M. O. dos; MORENO, I. |
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Afiliação: |
GUSTAVO DE MIRANDA CHAVES, ITAL; ALCINA MARIA LISERRE, ITAL; PATRÍCIA BLUMER ZACARCHENCO, ITAL; KARINA MARIA OLBRICH DOS SANTOS, Embrapa Caprinos e Ovinos (CNPC); IZILDINHA MORENO, ITAL. |
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Título: |
Simulação do sistema gastrintestinal humano para avaliação da resistência de probiótico em queijo de coalho com leite de cabra. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 3., 2009, Campinas. Anais... Campinas: ITAL: IAC; Jaguariúna: Embrapa Meio Ambiente, 2009. 6 f. 1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Resumo: Neste projeto, foram produzidos queijos com leite de cabra e probióticos para avaliação da resistência desses microrganismos à passagem pelo trato gastrintestinal simulado. Os queijos foram adicionados de Lactobacillus acidophilus e após 15 e 30 dias de estocagem refrigerada, amostras do queijo foram avaliadas para quantificação de L. acidophilus e sobrevivência dos probióticos à passagem simulada pelo sistema gastrintestinal humano. O suco gástrico com pH 2,5 foi adicionado de pepsina (3g/L). O suco entérico, com a adição de bile (3g/L), foi simulado com pH 5,6 e 7,5, de acordo com as condições do sistema intestinal humano. As contagens totais de L. acidophiluspara o queijo após 15 e 30 dias foram semelhantes, 7,03 e 7,04 logUFC/g, respectivamente. Considerando para consumo porção diária de 30g de queijo, obtémse um total de 108 células, o que atende às exigências da legislação brasileira para alimentos com alegações funcionais. Após o ensaio em condições simuladas do sistema gastrintestinal, as populações de bifidobactérias foram reduzidas a 3,79 e 4,33 logUFC/g para os queijos com 15 e 30 dias, respectivamente. Conclui-se que as contagens do probiótico após ação de suco gástrico e entérico formulados com pepsina e bile foram reduzidas em aproximadamente 3 ciclos logarítmicos, apesar da contagem inicial do queijo estar de acordo com a legislação brasileira. Abstract: In this study, goat milk cheeses with probiotic were produced and the survival of these bacteria after simulated conditions of human gastrointestinal tract was evaluated. The cheeses were added of Lactobacillus acidophilus and evaluated after 15 and 30 days of refrigerated storage. The gastric juice at pH 2.5 was added of pepsin (3g/L). The enteric juice added of bile (3g/L) was simulated with pH 5.6 and 7.5 according to the conditions of the duodenum, jejunum, ileum, and the large intestine. The counts of L. acidophilus were similar in the cheeses kept at 15 and 30 days, respectively 7,03 and 7,04 log CFU/g. These results show that the portion of 30g of cheese contains 108 cells. These data agree to the Brazilian legislation requirement related to the functional foods. After the assays simulating gastrointestinal conditions, the population of L. acidophilus were reduced to 3,79 and 4,33 log CFU/g in the cheese after 15 and 30 days of storage, respectively. It was observed that the population of probiotics after simulated conditions of gastric and enteric juices formulated with pepsin and bile, respectively, was reduced in approximately three logarithmic cycles, despite the initial counts attend to the brazilian legislation requirements. MenosResumo: Neste projeto, foram produzidos queijos com leite de cabra e probióticos para avaliação da resistência desses microrganismos à passagem pelo trato gastrintestinal simulado. Os queijos foram adicionados de Lactobacillus acidophilus e após 15 e 30 dias de estocagem refrigerada, amostras do queijo foram avaliadas para quantificação de L. acidophilus e sobrevivência dos probióticos à passagem simulada pelo sistema gastrintestinal humano. O suco gástrico com pH 2,5 foi adicionado de pepsina (3g/L). O suco entérico, com a adição de bile (3g/L), foi simulado com pH 5,6 e 7,5, de acordo com as condições do sistema intestinal humano. As contagens totais de L. acidophiluspara o queijo após 15 e 30 dias foram semelhantes, 7,03 e 7,04 logUFC/g, respectivamente. Considerando para consumo porção diária de 30g de queijo, obtémse um total de 108 células, o que atende às exigências da legislação brasileira para alimentos com alegações funcionais. Após o ensaio em condições simuladas do sistema gastrintestinal, as populações de bifidobactérias foram reduzidas a 3,79 e 4,33 logUFC/g para os queijos com 15 e 30 dias, respectivamente. Conclui-se que as contagens do probiótico após ação de suco gástrico e entérico formulados com pepsina e bile foram reduzidas em aproximadamente 3 ciclos logarítmicos, apesar da contagem inicial do queijo estar de acordo com a legislação brasileira. Abstract: In this study, goat milk cheeses with probiotic were produced and the survival of these bacteria af... Mostrar Tudo |
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Palavras-Chave: |
Alimento funcional; Enteric juice; Probiótico; Produto lácteo; Queijo de cabra. |
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Thesagro: |
Caprino; Leite de Cabra; Produto Derivado do Leite; Segurança Alimentar; Suco Digestivo; Suco Entérico; Suco Gástrico; Tecnologia de Alimento. |
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Thesaurus Nal: |
Digestive juices; Food safety; Food technology; Functional foods; Gastric acid; Goat cheese; Goat milk; Lactobacillus acidophilus; Probiotics. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/86463/1/AAC-Simulacao-do-sistema-gastrintestinal.pdf
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Marc: |
LEADER 04066nam a2200421 a 4500
001 1534464
005 2023-08-10
008 2009 bl uuuu u00u1 u #d
100 1 $aCHAVES, G. de M.
245 $aSimulação do sistema gastrintestinal humano para avaliação da resistência de probiótico em queijo de coalho com leite de cabra.$h[electronic resource]
260 $aIn: CONGRESSO INTERINSTITUCIONAL DE INICIAÇÃO CIENTÍFICA, 3., 2009, Campinas. Anais... Campinas: ITAL: IAC; Jaguariúna: Embrapa Meio Ambiente, 2009. 6 f. 1 CD-ROM.$c2009
520 $aResumo: Neste projeto, foram produzidos queijos com leite de cabra e probióticos para avaliação da resistência desses microrganismos à passagem pelo trato gastrintestinal simulado. Os queijos foram adicionados de Lactobacillus acidophilus e após 15 e 30 dias de estocagem refrigerada, amostras do queijo foram avaliadas para quantificação de L. acidophilus e sobrevivência dos probióticos à passagem simulada pelo sistema gastrintestinal humano. O suco gástrico com pH 2,5 foi adicionado de pepsina (3g/L). O suco entérico, com a adição de bile (3g/L), foi simulado com pH 5,6 e 7,5, de acordo com as condições do sistema intestinal humano. As contagens totais de L. acidophiluspara o queijo após 15 e 30 dias foram semelhantes, 7,03 e 7,04 logUFC/g, respectivamente. Considerando para consumo porção diária de 30g de queijo, obtémse um total de 108 células, o que atende às exigências da legislação brasileira para alimentos com alegações funcionais. Após o ensaio em condições simuladas do sistema gastrintestinal, as populações de bifidobactérias foram reduzidas a 3,79 e 4,33 logUFC/g para os queijos com 15 e 30 dias, respectivamente. Conclui-se que as contagens do probiótico após ação de suco gástrico e entérico formulados com pepsina e bile foram reduzidas em aproximadamente 3 ciclos logarítmicos, apesar da contagem inicial do queijo estar de acordo com a legislação brasileira. Abstract: In this study, goat milk cheeses with probiotic were produced and the survival of these bacteria after simulated conditions of human gastrointestinal tract was evaluated. The cheeses were added of Lactobacillus acidophilus and evaluated after 15 and 30 days of refrigerated storage. The gastric juice at pH 2.5 was added of pepsin (3g/L). The enteric juice added of bile (3g/L) was simulated with pH 5.6 and 7.5 according to the conditions of the duodenum, jejunum, ileum, and the large intestine. The counts of L. acidophilus were similar in the cheeses kept at 15 and 30 days, respectively 7,03 and 7,04 log CFU/g. These results show that the portion of 30g of cheese contains 108 cells. These data agree to the Brazilian legislation requirement related to the functional foods. After the assays simulating gastrointestinal conditions, the population of L. acidophilus were reduced to 3,79 and 4,33 log CFU/g in the cheese after 15 and 30 days of storage, respectively. It was observed that the population of probiotics after simulated conditions of gastric and enteric juices formulated with pepsin and bile, respectively, was reduced in approximately three logarithmic cycles, despite the initial counts attend to the brazilian legislation requirements.
650 $aDigestive juices
650 $aFood safety
650 $aFood technology
650 $aFunctional foods
650 $aGastric acid
650 $aGoat cheese
650 $aGoat milk
650 $aLactobacillus acidophilus
650 $aProbiotics
650 $aCaprino
650 $aLeite de Cabra
650 $aProduto Derivado do Leite
650 $aSegurança Alimentar
650 $aSuco Digestivo
650 $aSuco Entérico
650 $aSuco Gástrico
650 $aTecnologia de Alimento
653 $aAlimento funcional
653 $aEnteric juice
653 $aProbiótico
653 $aProduto lácteo
653 $aQueijo de cabra
700 1 $aLISERRE, A. M.
700 1 $aZACARHENCO, P. B.
700 1 $aSANTOS, K. M. O. dos
700 1 $aMORENO, I.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
11/11/2015 |
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Data da última atualização: |
17/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MUNERATO, M. S.; MARQUES, J. A.; CAULKETT, N. A.; TOMAS, W. M.; ZANETTI, E. S.; TROVATI, R. G.; PEREIRA, G. T.; PALME, R. |
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Afiliação: |
FCAV-UNESP; FCAV-UNESP; University of Calgary; WALFRIDO MORAES TOMAS, CPAP; FCAV-UNESP; ESALQ-USP; FCAV-UNESP; University of Veterinary Medicine Vienna. |
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Título: |
Hormonal and behavioural stress responses to capture and radio-collar fitting in free-ranging pampas deer (Ozotoceros bezoarticus). |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Animal Welfare, v. 24, p. 437-446, 2015. |
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DOI: |
10.7120/09627286.24.4.437 |
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Idioma: |
Inglês |
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Conteúdo: |
This study evaluated the short-term hormonal and behavioural responses to capture and radio-collar fitting in free-ranging pampas deer (Ozotoceros bezoarticus). Twenty adult deer (eleven females and nine males) were captured in the South Pantanal wetland (Brazil) and equipped with VHF radio-collars (marked deer). Untreated adult deer of the same sex were randomly chosen as the control group (nine females and nine males). On the day following capture, an observer followed all deer for faecal collection and behaviour evaluation. Faecal samples were immediately refrigerated and frozen at ?20°C within a maximum of 12 h. Faecal glucocorticoid metabolites (FGM) were measured using an 11-oxoaetiocholanolone enzyme immunoassay. A qualitative behaviour assessment and the consequences of capture were evaluated using pre-defined terminologies and scores. Flight distance was recorded using a range finder. FGM increased from 19?22!h after capture onwards and peak concentrations were five times (median) higher as the respective baseline values. FGM values of marked deer were significantly higher at 22?25 and 25-28!h compared with controls. Marked male but not female deer had significantly higher FGM values at 22?25 and 25?28!h compared with their baseline values. Marked deer were significantly more fearful, less sociable and defensive than controls. The absences of significant increases of FGM in the captured female deer may indicate that females are less prone to capture stress. The significantly more fearful, and less sociable and defensive patterns observed in marked deer may be relevant during capture of lactating females or in areas with high predator pressure. MenosThis study evaluated the short-term hormonal and behavioural responses to capture and radio-collar fitting in free-ranging pampas deer (Ozotoceros bezoarticus). Twenty adult deer (eleven females and nine males) were captured in the South Pantanal wetland (Brazil) and equipped with VHF radio-collars (marked deer). Untreated adult deer of the same sex were randomly chosen as the control group (nine females and nine males). On the day following capture, an observer followed all deer for faecal collection and behaviour evaluation. Faecal samples were immediately refrigerated and frozen at ?20°C within a maximum of 12 h. Faecal glucocorticoid metabolites (FGM) were measured using an 11-oxoaetiocholanolone enzyme immunoassay. A qualitative behaviour assessment and the consequences of capture were evaluated using pre-defined terminologies and scores. Flight distance was recorded using a range finder. FGM increased from 19?22!h after capture onwards and peak concentrations were five times (median) higher as the respective baseline values. FGM values of marked deer were significantly higher at 22?25 and 25-28!h compared with controls. Marked male but not female deer had significantly higher FGM values at 22?25 and 25?28!h compared with their baseline values. Marked deer were significantly more fearful, less sociable and defensive than controls. The absences of significant increases of FGM in the captured female deer may indicate that females are less prone to capture stress. The sign... Mostrar Tudo |
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Palavras-Chave: |
Enzyme immunoassay; Pampas deer; Qualitative behaviour assessment; Southern Pantanal wetland. |
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Thesagro: |
Hormônio animal. |
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Thesaurus NAL: |
Animal behavior; animal welfare; glucocorticoids; Hormones. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02643naa a2200325 a 4500
001 2028397
005 2016-03-17
008 2015 bl --- 0-- u #d
024 7 $a10.7120/09627286.24.4.437$2DOI
100 1 $aMUNERATO, M. S.
245 $aHormonal and behavioural stress responses to capture and radio-collar fitting in free-ranging pampas deer (Ozotoceros bezoarticus).
260 $c2015
520 $aThis study evaluated the short-term hormonal and behavioural responses to capture and radio-collar fitting in free-ranging pampas deer (Ozotoceros bezoarticus). Twenty adult deer (eleven females and nine males) were captured in the South Pantanal wetland (Brazil) and equipped with VHF radio-collars (marked deer). Untreated adult deer of the same sex were randomly chosen as the control group (nine females and nine males). On the day following capture, an observer followed all deer for faecal collection and behaviour evaluation. Faecal samples were immediately refrigerated and frozen at ?20°C within a maximum of 12 h. Faecal glucocorticoid metabolites (FGM) were measured using an 11-oxoaetiocholanolone enzyme immunoassay. A qualitative behaviour assessment and the consequences of capture were evaluated using pre-defined terminologies and scores. Flight distance was recorded using a range finder. FGM increased from 19?22!h after capture onwards and peak concentrations were five times (median) higher as the respective baseline values. FGM values of marked deer were significantly higher at 22?25 and 25-28!h compared with controls. Marked male but not female deer had significantly higher FGM values at 22?25 and 25?28!h compared with their baseline values. Marked deer were significantly more fearful, less sociable and defensive than controls. The absences of significant increases of FGM in the captured female deer may indicate that females are less prone to capture stress. The significantly more fearful, and less sociable and defensive patterns observed in marked deer may be relevant during capture of lactating females or in areas with high predator pressure.
650 $aAnimal behavior
650 $aanimal welfare
650 $aglucocorticoids
650 $aHormones
650 $aHormônio animal
653 $aEnzyme immunoassay
653 $aPampas deer
653 $aQualitative behaviour assessment
653 $aSouthern Pantanal wetland
700 1 $aMARQUES, J. A.
700 1 $aCAULKETT, N. A.
700 1 $aTOMAS, W. M.
700 1 $aZANETTI, E. S.
700 1 $aTROVATI, R. G.
700 1 $aPEREIRA, G. T.
700 1 $aPALME, R.
773 $tAnimal Welfare$gv. 24, p. 437-446, 2015.
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