| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
|
Data corrente: |
19/02/2021 |
|
Data da última atualização: |
07/02/2022 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Autoria: |
ARRAES, F. B. M.; MARTINS-DE-SA, D; VASQUEZ, D. D. N.; MELO, B. P.; FAHEEM, M.; MACEDO, L. L. P. de; MORGANTE, C. V.; BARBOSA, J. A. R. G.; TOGAWA, R. C.; MOREIRA, V. J. V.; DANCHIN, E. G. J.; SA, M. F. G. de. |
|
Afiliação: |
FABRICIO BARBOSA MONTEIRO ARRAES; DIOGO MARTINS-DE-SA; DANIEL D. NORIEGA VASQUEZ; BRUNO PAES MELO; MUHAMMAD FAHEEM; LEONARDO LIMA PEPINO DE MACEDO; CAROLINA VIANNA MORGANTE, CPATSA; JOÃO ALEXANDRE R. G. BARBOSA; ROBERTO COITI TOGAWA; VALDEIR JUNIO VAZ MOREIRA; ETIENNE G. J. DANCHIN; MARIA FATIMA GROSSI DE SA. |
|
Título: |
Dissecting protein domain variability in the core rna interference machinery of five insect orders. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
RNA Biology, v. 18, n. 11, p. 1653-1681, 2021. |
|
DOI: |
https://doi.org/10.1080/15476286.2020.1861816 |
|
Idioma: |
Inglês |
|
Conteúdo: |
RNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plants and viruses. In conclusion, our results highlight several variability hotspots that deserve further investigation in order to improve the application of RNAi technology in the control of insect pests. MenosRNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plant... Mostrar Tudo |
|
Palavras-Chave: |
Argonaute; DCR1; Dicer; Drosha; DsRBDs; Evolução da proteína; In silico analysis; Loquacious; Pasha; Protein evolution; R2D2; RIIID II; Sequência de proteínas inteira; Structure-function relationship. |
|
Thesagro: |
Controle Genético; Genoma; Inseto; Praga; Proteína. |
|
Thesaurus Nal: |
Insect control; Pest control; Protein structure; Proteins. |
|
Categoria do assunto: |
--
G Melhoramento Genético |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/230917/1/Dissecting-protein-domain-variability-2021.pdf
|
|
Marc: |
LEADER 03184naa a2200541 a 4500
001 2130159
005 2022-02-07
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1080/15476286.2020.1861816$2DOI
100 1 $aARRAES, F. B. M.
245 $aDissecting protein domain variability in the core rna interference machinery of five insect orders.$h[electronic resource]
260 $c2021
520 $aRNA interference (RNAi)-mediated gene silencing can be used to control specific insect pest populations. Unfortunately, the variable efficiency in the knockdown levels of target genes has narrowed the applicability of this technology to a few species. Here, we examine the current state of knowledge regarding the miRNA (micro RNA) and siRNA (small interfering RNA) pathways in insects and investigate the structural variability at key protein domains of the RNAi machinery. Our goal was to correlate domain variability with mechanisms affecting the gene silencing efficiency. To this end, the protein domains of 168 insect species, encompassing the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera, were analysed using our pipeline, which takes advantage of meticulous structure-based sequence alignments. We used phylogenetic inference and the evolutionary rate coefficient (K) to outline the variability across domain regions and surfaces. Our results show that four domains, namely dsrm, Helicase, PAZ and Ribonuclease III, are the main contributors of protein variability in the RNAi machinery across different insect orders. We discuss the potential roles of these domains in regulating RNAi-mediated gene silencing and the role of loop regions in fine-tuning RNAi efficiency. Additionally, we identified several order-specific singularities which indicate that lepidopterans have evolved differently from other insect orders, possibly due to constant coevolution with plants and viruses. In conclusion, our results highlight several variability hotspots that deserve further investigation in order to improve the application of RNAi technology in the control of insect pests.
650 $aInsect control
650 $aPest control
650 $aProtein structure
650 $aProteins
650 $aControle Genético
650 $aGenoma
650 $aInseto
650 $aPraga
650 $aProteína
653 $aArgonaute
653 $aDCR1
653 $aDicer
653 $aDrosha
653 $aDsRBDs
653 $aEvolução da proteína
653 $aIn silico analysis
653 $aLoquacious
653 $aPasha
653 $aProtein evolution
653 $aR2D2
653 $aRIIID II
653 $aSequência de proteínas inteira
653 $aStructure-function relationship
700 1 $aMARTINS-DE-SA, D
700 1 $aVASQUEZ, D. D. N.
700 1 $aMELO, B. P.
700 1 $aFAHEEM, M.
700 1 $aMACEDO, L. L. P. de
700 1 $aMORGANTE, C. V.
700 1 $aBARBOSA, J. A. R. G.
700 1 $aTOGAWA, R. C.
700 1 $aMOREIRA, V. J. V.
700 1 $aDANCHIN, E. G. J.
700 1 $aSA, M. F. G. de
773 $tRNA Biology$gv. 18, n. 11, p. 1653-1681, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Semiárido (CPATSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Suínos e Aves. |
|
Data corrente: |
26/07/2021 |
|
Data da última atualização: |
27/07/2021 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 2 |
|
Autoria: |
TEIXEIRA, S. A.; MARQUES, D. B. D.; COSTA, T. C.; OLIVEIRA, H. C.; COSTA, K. A.; CARRARA, E. R.; SILVA, W. da; GUIMARÃES, J. D.; NEVES, M. N.; IBELLI, A. M. G.; CANTAO, M. E.; LEDUR, M. C.; PEIXOTO, J. de O.; GUIMARÃES, S. E. F. |
|
Afiliação: |
SUSANA A. TEIXEIRA, UFV; DANIELE BOTELHO DINIZ MARQUES, UFV; THAÍS C. COSTA, UFV; HANIEL CEDRAZ DE OLIVEIRA, UFV; KARINE A. COSTA, UFV; EULA R. CARRARA, UFV; WALMIR DA SILVA, UFV; JOSÉ D. GUIMARÃES, UFV; MARIANA M. NEVES, UFV; ADRIANA MERCIA GUARATINI IBELLI, CNPSA; MAURICIO EGIDIO CANTAO, CNPSA; MONICA CORREA LEDUR, CNPSA; JANE DE OLIVEIRA PEIXOTO, CNPSA; SIMONE ELIZA FACIONI GUIMARÃES, UFV. |
|
Título: |
Transcription landscape of the early developmental biology in pigs. |
|
Ano de publicação: |
2021 |
|
Fonte/Imprenta: |
Animals, v. 11, n. 1443, 2021. |
|
DOI: |
https://doi.org/10.3390/ani11051443 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Abstract: Since pre- and postnatal development are programmed during early prenatal life, studies addressing the complete transcriptional landscape during organogenesis are needed. Therefore, we aimed to disentangle differentially expressed (DE) genes between fetuses (at 35 days old) and embryos (at 25 days old) through RNA-sequencing analysis using the pig as model. In total, 1705 genes were DE, including the top DE IBSP, COL6A6, HBE1, HBZ, HBB, and NEUROD6 genes, which are associated with developmental transition from embryos to fetuses, such as ossification, skeletal muscle development, extracellular matrix organization, cardiovascular system, erythrocyte differentiation, and neuronal system. In pathway analysis, embryonic development highlighted those mainly related to morphogenic signaling and cell interactions, which are crucial for transcriptional control during the establishment of the main organs in early prenatal development, while pathways related to myogenesis, neuronal development, and cardiac and striated muscle contraction were enriched for fetal development, according to the greater complexity of organs and body structures at this developmental stage. Our findings provide an exploratory and informative transcriptional landscape of pig organogenesis, which might contribute to further studies addressing specific developmental events in pigs and in other mammals. |
|
Palavras-Chave: |
Desenvolvimento pré-natal; RNA-seq. |
|
Thesagro: |
Organogénese; Reprodução Animal; Suíno. |
|
Thesaurus NAL: |
Organogenesis; Prenatal development; Swine. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 02456naa a2200385 a 4500
001 2133113
005 2021-07-27
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ani11051443$2DOI
100 1 $aTEIXEIRA, S. A.
245 $aTranscription landscape of the early developmental biology in pigs.$h[electronic resource]
260 $c2021
520 $aAbstract: Since pre- and postnatal development are programmed during early prenatal life, studies addressing the complete transcriptional landscape during organogenesis are needed. Therefore, we aimed to disentangle differentially expressed (DE) genes between fetuses (at 35 days old) and embryos (at 25 days old) through RNA-sequencing analysis using the pig as model. In total, 1705 genes were DE, including the top DE IBSP, COL6A6, HBE1, HBZ, HBB, and NEUROD6 genes, which are associated with developmental transition from embryos to fetuses, such as ossification, skeletal muscle development, extracellular matrix organization, cardiovascular system, erythrocyte differentiation, and neuronal system. In pathway analysis, embryonic development highlighted those mainly related to morphogenic signaling and cell interactions, which are crucial for transcriptional control during the establishment of the main organs in early prenatal development, while pathways related to myogenesis, neuronal development, and cardiac and striated muscle contraction were enriched for fetal development, according to the greater complexity of organs and body structures at this developmental stage. Our findings provide an exploratory and informative transcriptional landscape of pig organogenesis, which might contribute to further studies addressing specific developmental events in pigs and in other mammals.
650 $aOrganogenesis
650 $aPrenatal development
650 $aSwine
650 $aOrganogénese
650 $aReprodução Animal
650 $aSuíno
653 $aDesenvolvimento pré-natal
653 $aRNA-seq
700 1 $aMARQUES, D. B. D.
700 1 $aCOSTA, T. C.
700 1 $aOLIVEIRA, H. C.
700 1 $aCOSTA, K. A.
700 1 $aCARRARA, E. R.
700 1 $aSILVA, W. da
700 1 $aGUIMARÃES, J. D.
700 1 $aNEVES, M. N.
700 1 $aIBELLI, A. M. G.
700 1 $aCANTAO, M. E.
700 1 $aLEDUR, M. C.
700 1 $aPEIXOTO, J. de O.
700 1 $aGUIMARÃES, S. E. F.
773 $tAnimals$gv. 11, n. 1443, 2021.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Suínos e Aves (CNPSA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
