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Registro Completo |
Biblioteca(s): |
Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
Data corrente: |
02/01/2019 |
Data da última atualização: |
27/04/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SILVA, C. G. M.; RESENDE, A. V. de; MARTÍNEZ GUTIÉRREZ, A.; MOREIRA, S. G.; BORGHI, E.; ALMEIDA, G. O. |
Afiliação: |
Carine Gregório Machado Silva, Universidade Federal de Lavras - UFLA/Departamento de Agricultura; ALVARO VILELA DE RESENDE, CNPMS; Aarón Martínez Gutiérrez, Universidade Federal de São João del-Rei/Campus Sete Lagoas; Silvino Guimarães Moreira, Universidade Federal de Lavras - UFLA/Departamento de Agricultura; EMERSON BORGHI, CNPMS; Gabriela Oliveira Almeida, Universidade Federal de São João del-Rei/Campus Sete Lagoas. |
Título: |
Macronutrient uptake and export in transgenic corn under two levels of fertilization. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 53, n. 12, p. 1363-1372, dez. 2018. |
Idioma: |
Inglês |
Notas: |
Título em português: Absorção e exportação de macronutrientes em milho transgênico sob dois níveis de fertilização. |
Conteúdo: |
The objective of this work was to evaluate macronutrient uptake and export by transgenic corn hybrids under medium and high fertilization levels. For each fertilization level, a randomized complete block design was carried out in the summer crop season, under no-tillage and supplementary irrigation, with four cultivars and four replicates. Plants were sampled throughout the entire cycle and separated into leaves, stalk, husk, cob, and grains for the determination of dry matter mass and accumulation of macronutrients in the different growth stages. Under the high fertilization level, a greater dry matter mass and nutrient uptake were observed, but grain yield was similar to that under the medium fertilization level. The uptake of N, P, Ca, Mg, and S occurs until later in the cycle of the transgenic cultivars, which also showed lower concentrations of P, K, and S in grains, when compared to older hybrids. The differential behavior of the evaluated cultivars under different fertilization levels indicates the need of updating and improving nutritional management and fertilizer recommendations for the corn crop. |
Palavras-Chave: |
Alta produtividade; Consumo de luxo; Eficiência de uso de fertilizantes; Extração de nutrientes; Fertilizer use efficiency; High productivity; Luxury consumption; Nutrient absorption; Nutritional requirement. |
Thesagro: |
Exigência Nutricional; Zea Mays. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/189611/1/Macronutrient-uptake-and-export.pdf
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Marc: |
LEADER 02263naa a2200325 a 4500 001 2103673 005 2023-04-27 008 2018 bl uuuu u00u1 u #d 100 1 $aSILVA, C. G. M. 245 $aMacronutrient uptake and export in transgenic corn under two levels of fertilization.$h[electronic resource] 260 $c2018 500 $aTítulo em português: Absorção e exportação de macronutrientes em milho transgênico sob dois níveis de fertilização. 520 $aThe objective of this work was to evaluate macronutrient uptake and export by transgenic corn hybrids under medium and high fertilization levels. For each fertilization level, a randomized complete block design was carried out in the summer crop season, under no-tillage and supplementary irrigation, with four cultivars and four replicates. Plants were sampled throughout the entire cycle and separated into leaves, stalk, husk, cob, and grains for the determination of dry matter mass and accumulation of macronutrients in the different growth stages. Under the high fertilization level, a greater dry matter mass and nutrient uptake were observed, but grain yield was similar to that under the medium fertilization level. The uptake of N, P, Ca, Mg, and S occurs until later in the cycle of the transgenic cultivars, which also showed lower concentrations of P, K, and S in grains, when compared to older hybrids. The differential behavior of the evaluated cultivars under different fertilization levels indicates the need of updating and improving nutritional management and fertilizer recommendations for the corn crop. 650 $aExigência Nutricional 650 $aZea Mays 653 $aAlta produtividade 653 $aConsumo de luxo 653 $aEficiência de uso de fertilizantes 653 $aExtração de nutrientes 653 $aFertilizer use efficiency 653 $aHigh productivity 653 $aLuxury consumption 653 $aNutrient absorption 653 $aNutritional requirement 700 1 $aRESENDE, A. V. de 700 1 $aMARTÍNEZ GUTIÉRREZ, A. 700 1 $aMOREIRA, S. G. 700 1 $aBORGHI, E. 700 1 $aALMEIDA, G. O. 773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 53, n. 12, p. 1363-1372, dez. 2018.
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Embrapa Milho e Sorgo (CNPMS) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
11/02/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
PEREIRA, M. M.; RAPOSO, N. R. B.; BRAYNER, R.; TEIXEIRA, E. M.; OLIVEIRA, V.; QUINTAO, C. C. R.; CAMARGO, L. S. de A.; MATTOSO, L. H. C.; BRANDAO, H. de M. |
Afiliação: |
M. M. PEREIRA, UFJF; N. R. B. RAPOSO, UFJF; R. BRAYNER, University of Paris Diderot, Paris; E. M. TEIXEIRA; V. OLIVEIRA, UFJF; CAROLINA CAPOBIANGO ROMANO QUINTAO, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA; HUMBERTO DE MELLO BRANDAO, CNPGL. |
Título: |
Cytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Nanotechnology, v. 24, n. 7, 075103, 2013. |
DOI: |
https://doi.org/10.1088/0957-4484/24/7/075103 |
Idioma: |
Inglês |
Conteúdo: |
Cellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers. |
Palavras-Chave: |
Animals Apoptosis; Apoptosis/genetics; Cattle Cellulose/pharmacology; Drug effects; Fibroblasts/cytology; Fibroblasts/drug. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02323naa a2200301 a 4500 001 1979437 005 2024-02-05 008 2013 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1088/0957-4484/24/7/075103$2DOI 100 1 $aPEREIRA, M. M. 245 $aCytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers.$h[electronic resource] 260 $c2013 520 $aCellulose nanofibers (CNF) have mechanical properties that make them very attractive for applications in the construction of polymeric matrices, drug delivery and tissue engineering. However, little is known about their impact on mammalian cells. The objective of this study was to evaluate the cytotoxicity of CNF and their effect on gene expression of fibroblasts cultured in vitro. The morphology of CNF was analyzed by transmission electron microscopy and the surface charge by Zeta potential. Cell viability was analyzed by flow cytometry assay and gene expression of biomarkers focused on cell stress response such as Heat shock protein 70.1 (HSP70.1) and Peroxiredoxin 1 (PRDX1) and apoptosis as B-cell leukemia (BCL-2) and BCL-2 associated X protein (BAX) by RT-PCR assay. Low concentrations of CNF (0.02-100 μg ml(-1)) did not cause cell death; however, at concentrations above 200 μg ml(-1), the nanofibers significantly decreased cell viability (86.41 ± 5.37%). The exposure to high concentrations of CNF (2000 and 5000 μg ml(-1)) resulted in increased HSP70.1, PRDX1 and BAX gene expression. The current study concludes that, under the conditions tested, high concentrations (2000 and 5000 μg ml(-1)) of CNF cause decreased cell viability and affect the expression of stress- and apoptosis-associated molecular markers. 653 $aAnimals Apoptosis 653 $aApoptosis/genetics 653 $aCattle Cellulose/pharmacology 653 $aDrug effects 653 $aFibroblasts/cytology 653 $aFibroblasts/drug 700 1 $aRAPOSO, N. R. B. 700 1 $aBRAYNER, R. 700 1 $aTEIXEIRA, E. M. 700 1 $aOLIVEIRA, V. 700 1 $aQUINTAO, C. C. R. 700 1 $aCAMARGO, L. S. de A. 700 1 $aMATTOSO, L. H. C. 700 1 $aBRANDAO, H. de M. 773 $tNanotechnology$gv. 24, n. 7, 075103, 2013.
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