Data/hora: 23/06/2024 07:12:35

Biblioteca(s): | Embrapa Gado de Leite.
Data corrente: | 10/02/2017
Data da última atualização: | 30/01/2023
Autoria: | PAULA, C. M. P. de; SOUZA SOBRINHO, F. de; TECHIO, V. H.
Afiliação: | Cristina M. P. de Paula, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; Vania H. Techio, UFLA.
Título: | Chromosomal distribution of H3K4me2, H3K9me2 and 5-methylcytosine: variations associated with polyploidy and hybridization in Brachiaria (Poaceae).
Ano de publicação: | 2016
Fonte/Imprenta: | Plant Cell Reports, v. 35, n. 6, p. 1359-1369, 2016.
Idioma: | Inglês
Conteúdo: | Abstract Histone post-translational modifications and DNA methylation are epigenetic processes that are involved in structural and functional organization of the genome. This study compared the chromosomal distribution of modified histones and 5-methylcytosine (5-mCyt) in species and interspecific hybrids of Brachiaria with different ploidy levels and reproduction modes. The relation between H3K9me2 and 5-mCyt was observed in the nucleolus organizer region, centromeric central domain and pericentromeric region. H3K4me2 was detected in euchromatic domains, mainly in the terminal chromosomal regions. Comparison of chromosomal distribution among species and hybrids showed greater variation of chromosomal types for the H3K9me2 in B. decumbens (tetraploid and apomictic species) and the 963 hybrid, while, for the H3K4me2, the variation was higher in B. brizantha and B. decumbens (tetraploid and apomictic species) and 963 hybrid. The chromosome distribution of 5-mCyt was similar between B. brizantha and B. decumbens, which differ from the distribution observed in B. ruziziensis (diploid and sexual species). Significant alterations in DNA methylation were observed in the artificially tetraploidized B. ruziziensis and in the interspecific hybrids, possibly as result of hybridization and polyploidization processes. The monitoring of histone modifications and DNA methylation allowed categorizing nuclear and chromosomal distribution of these epigenetic marks, thus contributing to the knowledge of composition and structure of the genome/epigenome of Brachiaria species and hybrids. These data can be useful for speciation and genome evolution studies in genus Brachiaria, and represent important markers to explore relationships between genomes.
Palavras-Chave: | Epigenetics marks; Histone methylation; Interspecific hybrids.
Thesaurus Nal: | DNA methylation; Urochloa.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
Registro original: | Embrapa Gado de Leite (CNPGL)
Exemplares: | CNPGL - (23180-1) - UPC - AP - DD -  -  -  - DIS