Data/hora: 16/06/2024 00:09:05

Biblioteca(s): | Embrapa Uva e Vinho.
Data corrente: | 06/07/2022
Data da última atualização: | 06/09/2023
Autoria: | NICKEL, O.; FAJARDO, T. V. M.; TARGON, M. L. N. P.; MACHADO, M. A.
Afiliação: | OSMAR NICKEL, CNPUV; THOR VINICIUS MARTINS FAJARDO, CNPUV; M. L. N. P. TARGON, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira; M. A. MACHADO, Centro Avançado de Pesquisa Tecnológica do Agronegócio de Citros Sylvio Moreira.
Título: | Production and Use of Polyclonal Antibodies to the Coat Protein of Apple stem grooving virus expressed in Escherichia coli.
Ano de publicação: | 2004
Fonte/Imprenta: | Acta Horticulturae, v. 657, p. 35-40, ISHS 2004.
Idioma: | Inglês
Conteúdo: | The coat protein (cp) gene of Apple stem grooving Capillovirus was amplified by RT-PCR, cloned, sequenced and subcloned in the expression plasmid pMal-c2. The ASGV cp gene was expressed in E. coli as a fusion protein (fp) containing a fragment of E. coli maltose binding protein (MBP). Bacterial cells were disrupted by sonication and the ASGVcp/MBP-fp was purified by amylose resin affinity chromatography. Polyclonal antibodies from rabbits immunized with the fp, gave specific reactions to ASGV from several infected apple cultivars at dilutions of up to 1:2000 in indirect ELISA. The ASGVcp/MBP-fp reacted to antisera raised against it as well as to commercial antisera against ASGV virions in immunoblotting. These results are a fundamental contribution to the development of an efficient ASGVindexing of propagative and mother stock materials in Southern Brazil.
Palavras-Chave: | ASGV; Recombinant viral antigen.
Thesagro: | Elisa.
Thesaurus Nal: | Antibodies; Apples; Recombinant antigens.
Categoria do assunto: | F Plantas e Produtos de Origem Vegetal
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144491/1/Nickel-ISHS-657-2003-p35-40.pdf
Registro original: | Embrapa Uva e Vinho (CNPUV)
Exemplares: | CNPUV - (18839-1) - UPC - AA - DD -  -  -  - DIS