Data/hora: 08/06/2024 01:57:56

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 18/10/2018
Data da última atualização: | 18/10/2018
Autoria: | RIBEIRO JUNIOR, J. C.; TEIDER JUNIOR, P. I.; OLIVEIRA, A. L. M.; RIOS, E. A.; TAMANINI, R.; BELOTI, V.
Afiliação: | José C. Ribeiro Júnior; Pedro I. Teider Junior; André L. M. Oliveira; Edson A. Rios; Ronaldo Tamanini; Vanerli Beloti.
Título: | Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk.
Ano de publicação: | 2018
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 38, n 8, p. 1577-1583, agosto 2018
Idioma: | Inglês
Notas: | Título em português: Proteolytic and lipolytic potential of Pseudomonas spp. from goat and bovine raw milk.
Conteúdo: | Pseudomonas, the main genus of gram-negative microorganisms isolated from milk, is psychrotrophic, biofilm-forming, and thermo-resistant deteriorating enzyme producers. The aim of this study was to quantify Pseudomonas spp. in goat?s and cow?s milk produced in the Paraná state, Brazil, to evaluate the deteriorating activity of the isolates at mesophilic and psychrotrophic conditions and to identify, at the species level, the isolates with alkaline metalloprotease (aprX gene) production potential. Microbiological, biochemical and molecular methods were used for isolating, confirming and identifying of isolates. The mean counts were 1.6 (±6.3)x104 and 0.89(±3)x102 CFU/mL for goat and bovine milk samples, respectively, immediately after milking. Of the Pseudomonas colonies isolated from goat milk (n=60), 91.7% showed proteolytic potential when incubated at 35°C/48 h and 80% at 7°C/10 days, and lipolytic potential was observed in 95% of the isolates incubated in mesophilic and 78.3% at refrigeration conditions. From the isolates of bovine milk (n=20), 35% showed proteolytic activity only when incubated at 35°C/48 h, and lipolytic potential was observed in 25% of the isolates incubated at 7°C/10d and 35°C/48h. It was observed that 83.3% and 25% of the isolates genetically confirmed as Pseudomonas spp. of goat and bovine milk showed the potential for alkaline metalloprotease production, with the species P. azotoformans, P. koreensis, P. gessardii, P. monteilii and P. lurida being the most frequent in goat milk and P. aeruginosa the only species identified in cow milk.
Palavras-Chave: | Alkaline metalloprotease; Bacterioses; Bovine; Deteriorante; Diversidade microbiana; Lipolytic; Microbial diversity; Proteolítico; Pseudomonas spp.
Thesagro: | Bacteriose; Bovino; Caprino; Leite.
Thesaurus Nal: | Goats; Milk; Spoilage.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/184709/1/Proteolytic-and-lipolytic-potential.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (63203-1) - UPE - AP - DD -  -  -  - DIS