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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
04/07/2019 |
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Data da última atualização: |
04/07/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAITANO-BERTOLACCI, M. A. B.; BASTOS, R. R. P.; SANTOS, L. R. dos; SOARES, C. O.; RIEGER, J. S. G.; MANTOVANI, C.; SANTANA, M. de S.; ROSINHA, G. M. S. |
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Afiliação: |
Universidade Federal de Mato Grosso do Sul/FUFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária e Zootecnia; LENITA RAMIRES DOS SANTOS, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Ciências Farmacêuticas, Alimentos e Nutrição; CNPGC; GRACIA MARIA SOARES ROSINHA, CNPGC. |
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Título: |
DNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 18, n. 2, gmr18295, 2019 |
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Idioma: |
Inglês |
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Conteúdo: |
VIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection. MenosVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellu... Mostrar Tudo |
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Palavras-Chave: |
DNA vaccine; T4SS; VirB operon; VirB12; VirB9. |
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Thesagro: |
Brucella Abortus. |
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Thesaurus Nal: |
Bovine brucellosis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02847naa a2200289 a 4500
001 2110376
005 2019-07-04
008 2019 bl uuuu u00u1 u #d
100 1 $aCAITANO-BERTOLACCI, M. A. B.
245 $aDNA vaccine and DNA prime-protein boost with the virB9 and virB12 genes induced low level of protection against Brucella abortus infection in mice.$h[electronic resource]
260 $c2019
520 $aVIRB proteins from Brucella spp. constitute the type IV Secretion System (T4SS), a key virulence factor that mediates the intracellular survival of these bacteria. We investigated the immunogenicity and protection of proteins produced by the virB9 and virB12 genes in the DNA vaccine and DNA prime-protein boost strategies. Groups of 10 mice were vaccinated with pcDNAvirB9, pcDNAvirB12, pcDNAvirB9+rVIRB9 or pcDNAvirB12+rVIRB12. The latter two groups were vaccinated with the proteins rVIRB9 and rVIRB12, respectively, during the third immunization. Three weeks after the last immunization, six animals from each group were challenged intraperitoneally with B. abortus strain S2308, and the efficacy of the vaccines was calculated as the log10 of protection by subtracting the mean log CFU of the vaccinated group from the mean log CFU of the negative control group (injected with sterile saline). Most of the vaccinated mice produced total IgG and the subclasses IgG1 and IgG2a against the respective protein, except for the mice vaccinated with pcDNAvirB12. Cytokines IFN-γ and IL-10 were produced, but without a significant difference between the vaccinated and negative control groups. The vaccines did not induce significant levels of protection, in contrast to the immunization obtained with the S19 vaccine strain (Log10, 1.48). In conclusion, the virB9 and virB12 genes of B. abortus, using DNA vaccine and DNA prime-protein boost strategies, were able to induce both humoral and cellular immune responses, but not enough to induce significant protection in the immunized mice. However, given the response in this system, further investigations using the virB9 and virB12 genes of Brucella spp., together with different immune modulators, are warranted. An effort should be made to direct and enhance the immune response, in order to identify a combination that stimulates a better immune response and, consequently, a better level of protection.
650 $aBovine brucellosis
650 $aBrucella Abortus
653 $aDNA vaccine
653 $aT4SS
653 $aVirB operon
653 $aVirB12
653 $aVirB9
700 1 $aBASTOS, R. R. P.
700 1 $aSANTOS, L. R. dos
700 1 $aSOARES, C. O.
700 1 $aRIEGER, J. S. G.
700 1 $aMANTOVANI, C.
700 1 $aSANTANA, M. de S.
700 1 $aROSINHA, G. M. S.
773 $tGenetics and Molecular Research$gv. 18, n. 2, gmr18295, 2019
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Embrapa Gado de Corte (CNPGC) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Territorial. Para informações adicionais entre em contato com cnpm.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Territorial. |
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Data corrente: |
15/03/2021 |
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Data da última atualização: |
15/03/2021 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
TEIXEIRA, A. H. DE. C.; LEIVAS, J. F.; PACHECO, E. P.; GARCON, E. A. M.; TAKEMURA, C. M. |
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Afiliação: |
ANTÔNIO HERIBERTO DE CASTRO TEIXEIRA, UFS; JANICE FREITAS LEIVAS, CNPM; EDSON PATTO PACHECO, CPATC; EDLENE APARECIDA MONTEIRO GARCON, CNPM; CELINA MAKI TAKEMURA, CNPM. |
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Título: |
Biophysical Characterization and Monitoring Large-Scale Water and Vegetation Anomalies by Remote Sensing in the Agricultural Growing Areas of the Brazilian Semi-Arid Region. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
In: PANDEY, P. C.; LAXMI, K. S. (Ed.). Advances in Remote Sensing for Natural Resource Monitoring. Nova Jersey, EUA: John Wiley & Sons, 2021. |
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Páginas: |
p. 94-109. |
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Idioma: |
Português |
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Conteúdo: |
The objective of this researchwas to test the operationality of coupling long series MODIS reflectance images and agrometeorological data for biophysical characterizations and further analyses of anomalies on water and vegetation conditions. The mixed weather and remote sensing parameters used were ET, BIO, and P at a 16-day period and 250m spatial scale, considering the period from 2003 to 2017. The counties of Petrolina and Juazeiro, Pernambuco and Bahia states, respectively, in the semi-arid of northeast Brazil, are considered the reference regions for semi-arid environments experiencing climate instability and land-use changes. |
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Palavras-Chave: |
Water and vegetation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01399naa a2200193 a 4500
001 2130690
005 2021-03-15
008 2021 bl uuuu u00u1 u #d
100 1 $aTEIXEIRA, A. H. DE. C.
245 $aBiophysical Characterization and Monitoring Large-Scale Water and Vegetation Anomalies by Remote Sensing in the Agricultural Growing Areas of the Brazilian Semi-Arid Region.$h[electronic resource]
260 $c2021
300 $ap. 94-109.
520 $aThe objective of this researchwas to test the operationality of coupling long series MODIS reflectance images and agrometeorological data for biophysical characterizations and further analyses of anomalies on water and vegetation conditions. The mixed weather and remote sensing parameters used were ET, BIO, and P at a 16-day period and 250m spatial scale, considering the period from 2003 to 2017. The counties of Petrolina and Juazeiro, Pernambuco and Bahia states, respectively, in the semi-arid of northeast Brazil, are considered the reference regions for semi-arid environments experiencing climate instability and land-use changes.
653 $aWater and vegetation
700 1 $aLEIVAS, J. F.
700 1 $aPACHECO, E. P.
700 1 $aGARCON, E. A. M.
700 1 $aTAKEMURA, C. M.
773 $tIn: PANDEY, P. C.; LAXMI, K. S. (Ed.). Advances in Remote Sensing for Natural Resource Monitoring. Nova Jersey, EUA: John Wiley & Sons, 2021.
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