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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
07/03/2014 |
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Data da última atualização: |
20/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
REIS, R. R.; DIAS, B. B. A.; MARTINS, P. K.; MARTINS, M. T. B.; ALEKCEVETCH, J. C.; JÚNIOR, A. C.; ANDRADE, A. C.; RIBEIRO, A. P.; QIND, F.; MIZOI, J.; SHINOZAKI, K. I.; NAKASHIMA, K.; CARVALHO, J. de F. C.; SOUSA, C. A. F. de; NEPOMUCENO, A. L.; KOBAYASHI, A. K.; MOLINARI, H. B. C. |
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Afiliação: |
Rafaela Ribeiro Reis, Universidade Federal de Lavras; BARBARA ANDRADE DIAS BRITO DA CUNHA, CNPAE; Polyana Kelly Martins; Maria Thereza Bazzo Martins; Jean Carlos Alekcevetch, Universidade Federal de Lavras; Antônio Chalfun-Júnior, Universidade Federal de Lavras; ALAN CARVALHO ANDRADE, CENARGEN; Ana Paula Ribeiro, Universidade Federal de Lavras; Feng Qind, Chinese Academy of Sciences; Junya Mizoi, The University of Tokyo; Kazuko Yamaguchi-Shinozaki, The University of Tokyo; Kazuo Nakashima, Chinese Academy of Sciences; Josirley de Fátima Corrêa Carvalho; CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; ALEXANDRE LIMA NEPOMUCENO, SRI; ADILSON KENJI KOBAYASHI, CNPAE; HUGO BRUNO CORREA MOLINARI, CNPAE. |
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Título: |
Induced over-expression of AtDREB2A CA improves drought tolerancein sugarcane. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Science, v. 221-222, p. 59-68, 2014. |
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DOI: |
10.1016/j.plantsci.2014.02.003 |
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Idioma: |
Inglês |
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Conteúdo: |
tDrought is one of the most challenging agricultural issues limiting sustainable sugarcane production and,in some cases, yield losses caused by drought are nearly 50%. DREB proteins play vital regulatory rolesin abiotic stress responses in plants. The transcription factor DREB2A interacts with a cis-acting DREsequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in Arabidopsis thaliana. In the present study, we evaluated the effects of stress-inducibleover-expression of AtDREB2A CA on gene expression, leaf water potential (L), relative water content(RWC), sucrose content and gas exchanges of sugarcane plants submitted to a four-days water deficittreatment in a rhizotron-grown root system. The plants were also phenotyped by scanning the roots andmeasuring morphological parameters of the shoot. The stress-inducible expression of AtDREB2A CA intransgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. Thetransgenic plants maintained higher RWC and Lover 4 days after withholding water and had higherphotosynthetic rates until the 3rd day of water-deficit. Induced expression of AtDREB2A CA in sugarcaneincreased sucrose levels and improved bud sprouting of the transgenic plants. Our results indicate thatinduced expression of AtDREB2A CA in sugarcane enhanced its drought tolerance without biomass penalty. |
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Palavras-Chave: |
AtDREB2A CA; Saccharum spp; Transcription factor. |
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Thesaurus Nal: |
abiotic stress. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02480naa a2200373 a 4500
001 1981800
005 2017-09-20
008 2014 bl uuuu u00u1 u #d
024 7 $a10.1016/j.plantsci.2014.02.003$2DOI
100 1 $aREIS, R. R.
245 $aInduced over-expression of AtDREB2A CA improves drought tolerancein sugarcane.$h[electronic resource]
260 $c2014
520 $atDrought is one of the most challenging agricultural issues limiting sustainable sugarcane production and,in some cases, yield losses caused by drought are nearly 50%. DREB proteins play vital regulatory rolesin abiotic stress responses in plants. The transcription factor DREB2A interacts with a cis-acting DREsequence to activate the expression of downstream genes that are involved in drought-, salt- and heat-stress response in Arabidopsis thaliana. In the present study, we evaluated the effects of stress-inducibleover-expression of AtDREB2A CA on gene expression, leaf water potential (L), relative water content(RWC), sucrose content and gas exchanges of sugarcane plants submitted to a four-days water deficittreatment in a rhizotron-grown root system. The plants were also phenotyped by scanning the roots andmeasuring morphological parameters of the shoot. The stress-inducible expression of AtDREB2A CA intransgenic sugarcane led to the up-regulation of genes involved in plant response to drought stress. Thetransgenic plants maintained higher RWC and Lover 4 days after withholding water and had higherphotosynthetic rates until the 3rd day of water-deficit. Induced expression of AtDREB2A CA in sugarcaneincreased sucrose levels and improved bud sprouting of the transgenic plants. Our results indicate thatinduced expression of AtDREB2A CA in sugarcane enhanced its drought tolerance without biomass penalty.
650 $aabiotic stress
653 $aAtDREB2A CA
653 $aSaccharum spp
653 $aTranscription factor
700 1 $aDIAS, B. B. A.
700 1 $aMARTINS, P. K.
700 1 $aMARTINS, M. T. B.
700 1 $aALEKCEVETCH, J. C.
700 1 $aJÚNIOR, A. C.
700 1 $aANDRADE, A. C.
700 1 $aRIBEIRO, A. P.
700 1 $aQIND, F.
700 1 $aMIZOI, J.
700 1 $aSHINOZAKI, K. I.
700 1 $aNAKASHIMA, K.
700 1 $aCARVALHO, J. de F. C.
700 1 $aSOUSA, C. A. F. de
700 1 $aNEPOMUCENO, A. L.
700 1 $aKOBAYASHI, A. K.
700 1 $aMOLINARI, H. B. C.
773 $tPlant Science$gv. 221-222, p. 59-68, 2014.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
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Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
16/09/2014 |
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Data da última atualização: |
13/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PAULA, D. P.; ANDOW, D. A.; TIMBÓ, R. V.; SUJII, E. R.; PIRES, C. S. S. |
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Afiliação: |
DEBORA PIRES PAULA, CENARGEN; DAVID A. ANDOW, UNIVERSITY OF MINNESOTA, USA; RENATA VELOZO TIMBÓ, UNB; EDISON RYOITI SUJII, CENARGEN; CARMEN SILVIA SOARES PIRES, CENARGEN. |
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Título: |
Uptake and transfer of a Bt toxin by a lepidoptera to Its eggs and effects on Its offspring. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
PLoS ONE, v. 9, n. 4, 2014. (Open access). |
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Idioma: |
Inglês |
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Conteúdo: |
Research on non-target effects of transgenic crop plants has focused primarily on bitrophic, tritrophic and indirect effects of entomotoxins from Bacillus thuringiensis, but little work has considered intergenerational transfer of Cry proteins. This work reports a lepidopteran (Chlosyne lacinia) taking up a Bt entomotoxin when exposed to sublethal or low concentrations, transferring the entomotoxin to eggs, and having adverse effects on the first filial generation (F1) offspring. Two bioassays were conducted using a sublethal concentration of toxin (100.0 ng/ml Cry1Ac) for adults and a concentration equal to the LC10 (2.0 ng/ml Cry1Ac) for larvae. Cry1Ac is the most common entomotoxin expressed in Bt cotton in Brazil. In the adult diet bioassay there was no adverse effect on the parental generation (P0) adults, but the F1 larvae had higher mortality and longer development time compared to F1 larvae of parents that did not ingest Cry1Ac. For the 3rd instar larvae, there was no measurable effect on the P0 larvae, pupae and adults, but the F1 larvae had higher mortality and longer development time. Using chemiluminescent Western Blot, Cry1Ac was detected in F1 eggs laid by P0 butterflies from both bioassays. Our study indicates that, at least for this species and these experimental conditions, a ,65 kDa insecticidal protein can be taken up and transferred to descendants where it can increase mortality and development time. |
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Thesaurus NAL: |
Lepidoptera. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/180016/1/pone.0095422.pdf
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Marc: |
LEADER 01971naa a2200181 a 4500
001 1995058
005 2023-03-13
008 2014 bl uuuu u00u1 u #d
100 1 $aPAULA, D. P.
245 $aUptake and transfer of a Bt toxin by a lepidoptera to Its eggs and effects on Its offspring.$h[electronic resource]
260 $c2014
520 $aResearch on non-target effects of transgenic crop plants has focused primarily on bitrophic, tritrophic and indirect effects of entomotoxins from Bacillus thuringiensis, but little work has considered intergenerational transfer of Cry proteins. This work reports a lepidopteran (Chlosyne lacinia) taking up a Bt entomotoxin when exposed to sublethal or low concentrations, transferring the entomotoxin to eggs, and having adverse effects on the first filial generation (F1) offspring. Two bioassays were conducted using a sublethal concentration of toxin (100.0 ng/ml Cry1Ac) for adults and a concentration equal to the LC10 (2.0 ng/ml Cry1Ac) for larvae. Cry1Ac is the most common entomotoxin expressed in Bt cotton in Brazil. In the adult diet bioassay there was no adverse effect on the parental generation (P0) adults, but the F1 larvae had higher mortality and longer development time compared to F1 larvae of parents that did not ingest Cry1Ac. For the 3rd instar larvae, there was no measurable effect on the P0 larvae, pupae and adults, but the F1 larvae had higher mortality and longer development time. Using chemiluminescent Western Blot, Cry1Ac was detected in F1 eggs laid by P0 butterflies from both bioassays. Our study indicates that, at least for this species and these experimental conditions, a ,65 kDa insecticidal protein can be taken up and transferred to descendants where it can increase mortality and development time.
650 $aLepidoptera
700 1 $aANDOW, D. A.
700 1 $aTIMBÓ, R. V.
700 1 $aSUJII, E. R.
700 1 $aPIRES, C. S. S.
773 $tPLoS ONE$gv. 9, n. 4, 2014. (Open access).
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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