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| Registros recuperados : 59 | |
| 21. |  | LAHMAR, R.; ARRÚE, J. L.; DENARDIN, J. E.; GUPTA, R. K.; RIBEIRO, M. F. S.; TOURDONNET, S. de. Knowledge assessment and sharing on sustainable agriculture. Main results, gaps in knowledge and challenges in the Latin American platform. In: LAHMAR, R.; ARRÚE, J. L.; DENARDIN, J. E.; GUPTA, R. K.; RIBEIRO, M. F. S.; TOURDONNET, S. de. (Ed.). KASSA - Knowledge Assessment and Sharing on Sustainable Agriculture. Montpellier: CIRAD, 2007. 6 p. 1 CD-ROM. Projeto DG-Research - Contract nº GOCE-CT-2004-505582.| Biblioteca(s): Embrapa Trigo. |
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| 22. | | TOMICH, R. G. P.; PELLEGRIN, A. O.; SERRA, C. V.; CURADO, F. F.; RIBEIRO, M. F. B.; STANCIOLI, E. F. Estudo participativo na avaliação do processo saúde-doença de bovinos em assentamentos rurais de Corumbá, MS. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 23., 2005, Santos, SP. Resumos. Santos: Sociedade Brasileira de Microbiologia, 2005. CD-ROM.| Biblioteca(s): Embrapa Pantanal. |
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| 23. | | VIEIRA, L. da S.; LIMA, J. D.; RIBEIRO, M. F. B.; BOZZI, I. A.; CAMARGOS, E. R. S. Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimoff & Rastegaieff, 1930 Emend. Levine, 1961 in experimentally infected goat. Memória do Instituto Oswaldo Cruz, v. 92, n. 4, p. 533-538, jul./ago. 1997.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 24. | | VIEIRA, L. da S.; LIMA, J. D.; RIBEIRO, M. F. B.; BOZZI, I. A.; CAMARGOS, E. R. S. Ultrastructure of endogenous stages of Eimeria ninakohlyakimovae Yakimov & Rategaieff, 1930 Emend. Levine, 1961 in experimentally infected goat. In: CONGRESSO PANAMERICANO DE CIÊNCIAS VETERINÁRIAS, 15., 1996, Campo Grande. Resumos... Campo Grande: Associacao Panamericana de Ciencias Veterinarias, 1996. p. 341. Ref. 242.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 26. | | MUZILLI, O. LUGÃO, S. M. B, FIDALSKI, J. JR. D. S, RIBEIRO, M. F. S, FAGUNDES, A. C. Adubação verde para a melhoria dos solos ocupados com lavouras caffeiras na região do Arenito Caiuá, resultudos preliminares, safra 1989/1990. Londrina: IAPAR, 1992. 14 p. (IAPAR. Informe da Pesquisa, 101).| Biblioteca(s): Embrapa Agrobiologia. |
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| 27. | | MATOS, A. T. de; VIDIGAL, S. M.; SEDIYAMA, M. A. N.; GARCIA, N. C. P.; RIBEIRO, M. F. Compostagem de alguns residuos organicos, utilizando-se aguas residuarias da suinocultura como fonte de nitrogenio. Revista Brasileira de Engenharia Agricola e Ambiental, Campina Grande, v.2,n.2,p.199-203,1998.| Biblioteca(s): Embrapa Algodão. |
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| 28. | | MATOS, A. T. de; SEDIYAMA, M. A. N.; VIDIGAL, S. M.; GARCIA, N. C. P.; RIBEIRO, M. F. Compostos orgânicos contendo dejeto liquido de suinos como fontes de nitrogênio: I- dinâmica da compostagem. IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995. v.2, p. 663-665.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 30. | | MENDES, C. Q.; SUSIN, I.; PIRES, A. V.; NUSSIO, R. C.; ARAUJO, M. F.; RIBEIRO, M. F. Desempenho, parâmetros da carcaça e comportamento ingestivo de cordeiros alimentados com cana-de-açúcar ensilada ou in natura. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 60, n. 3, p. 733-740, abr. 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 31. | | LIMA, C. B. S.; NUNES, L. A.; CARVALHO, C. A. L.; RIBEIRO, M. F.; SOUZA, B. A. Morphometric differences and fluctuating asymmetry in Melipona subnitida Ducke 1910 (Hymenoptera: Apidae) in different types of housing. Brazilian Journal of Biology, São Carlos, v. 76, n. 4, p. 845-850, 2016. Autoria: MARCIA [i.e. DE] FATIMA RIBEIRO; BRUNO [ i.e. DE] ALMEIDA SOUZA.| Biblioteca(s): Embrapa Meio-Norte. |
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| 32. | | ANDRADE, N. P. C.; SILVA, E. M. S. da; MOTA, R. A.; VESCHI, J. L. A.; RIBEIRO, M. F.; KREWER, C. C.; COSTA, M. M. da. Atividade antimicrobiana in vitro de extratos etanólicos de própolis de três estados brasileiros sobre Aeromonas hydrophila isoladas de peixes. Arquivos do Instituto Biológico, São Paulo, v. 79, n. 1, p. 9-15, 2012.| Biblioteca(s): Embrapa Semiárido. |
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| 33. | | TOMICH, R. G. P.; PELLEGRIN, A. O.; SERRA, C. V.; RIBEIRO, M. F. B.; PASSOS, L. M. F.; BARBOSA-STANCIOLI, E. F. Estudo de doenças bovinas em assentamentos rurais do Município de Corumbá, MS - resultados iniciais. In: SIMPÓSIO SOBRE RECURSOS NATURAIS E SÓCIO-ECONÔMICOS DO PANTANAL, 4., 2004, Corumbá, MS. Sustentabilidade regional: anais. Corumbá: Embrapa Pantanal: UCDB: UFMS: SEBRAE-MS, 2004. (CD-ROM).| Biblioteca(s): Embrapa Pantanal. |
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| 34. | | RODRIGUES, G. H.; SUSIN, I.; PIRES, A. V.; MENDES, C. Q.; ARAUJO, R. C. de; PACKER, I. U.; RIBEIRO, M. F.; GERAGE, L. V. Substituição do milho por polpa cítrica em rações com alta proporção de concentrado para cordeiros confinados. Ciência Rural, Santa Maria, v. 38, n. 3, p. 789-794, mai./jun., 2008.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 35. | | JORDAN, C.; SANTOS, P. L. dos; OLIVEIRA, L. R. dos S.; DOMINGUES, M. M.; GÊA, B. C. C.; RIBEIRO, M. F.; MASCARIN, G. M.; WILCKEN, C. F. Entomopathogenic fungi as the microbial frontline against the alien Eucalyptus pest Gonipterus platensis in Brazil. Scientific Reports, v. 11, article 7233, 2021.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 36. | | PEREIRA, H. D.; SIMÕES, S. V. D.; SILVEIRA, J. A. G.; RIBEIRO, M. F. B.; CADIOLI, F. A.; SAMPAIO, P. H. Aspectos clínicos, epidemiológicos e diagnóstico da infecção por Trypanosoma vivax em rebanho bovino no estado do Maranhão. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 5, p. 896-901, maio 2018 Título em inglês: Clinical and epidemiological aspects and diagnosis of Trypanosoma vivax infection in a cattle herd, state of Maranhão, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 37. | | RIBEIRO, M. F.; MALAGODI-BRAGA, K. S.; CAMARGO, C. C. F. de; CANUTO, J. C.; MORICONI, W.; SANTOS, J. J.; CÓL, L. Z. da. Avaliação da reprodução de Crotalaria spectabilis e C. juncea. In: SIMPÓSIO BRASILEIRO DE POLINIZAÇÃO, 2., 2016, Catalão. Anais... Catalão: Universidade Federal de Goiás, 2016. Pôster. p. 93.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 38. | | ARAUJO, A. C.; SILVEIRA, J. A. G.; AZEVEDO, S. S.; NIERI-BASTOS, F. A.; RIBEIRO, M. F. B.; LABRUNA, M. B.; HORTA, M. C. Babesia canis vogeli infection in dogs and ticks in the semiarid region of Pernanbuco, Brazil. Pesquisa Veterinária Brasileira, Rio de Janeiro v. 35, n. 5, p. 456-461, maio 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 39. | | ARAÚJO, R. C. de; PIRES, A. V.; SUSIN, I.; MENDES, C. Q.; RODRIGUES, G. H.; GERAGE, L. V.; RIBEIRO, M. F. Comportamento ingestivo de ovelhas Santa Inês em lactação alimentadas com casca de soja substituindo o feno de "coastcross" (Cynodon sp). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 42., 2005, Goiânia. A produção animal e o foco no agronegócio: anais. Goiânia: Sociedade Brasileira de Zootecnia, 2005. 4 f. CD ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 40. | | RODRIGUES, G. H.; SUSIN, I.; PIRES, A. V.; MENDESM, C. Q.; URANO, F. S.; ARAÚJO, R. C. de; RIBEIRO, M. F.; GERAGE, L. V. Digestiblidade aparente dos nutrientes no trato digestório de cordeiros alimentados com dietas contendo polpa cítrica. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 42., 2005, Goiânia. A produção animal e o foco no agronegócio: anais. Goiânia: Sociedade Brasileira de Zootecnia, 2005. 5 f. CD ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 59 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
05/01/2012 |
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Data da última atualização: |
03/04/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
KULCHESKI, F. R.; OLIVEIRA, L. F. V.; MOLINA, L. G.; ALMERAO, M. P.; RODRIGUES, F. A.; MARCOLINO, J.; BARBOSA, J. F.; STOLF-MOREIRA, R.; NEPOMUCENO, A. L.; MARCELINO-GUIMARÃES, F. C.; ABDELNOOR, R. V.; NASCIMENTO, L. C.; CARAZZOLLE, M. F.; PEREIRA, G. A. G.; MARGIS, R. |
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Afiliação: |
FRANCELI R. KULCHESKI, UFRGS; LUIZ F. V. DE OLIVEIRA, UFRGS; LORRAYNE, G. MOLINA, UFRGS; MAURÍCIO P. ALMERÃO, UFRGS; FABIANA A. RODRIGUES, CNPSo; JULIANA MARCOLINO, CNPSo; JOICE F. BARBOSA, CNPSo; RENATA STOLF MOREIRA, CNPSo; ALEXANDRE LIMA NEPOMUCENO, SRI; FRANCISMAR CORREA MARCELINO GUIMARÃES, CNPSO; RICARDO VILELA ABDELNOOR, CNPSO; LEANDRO C. NASCIMENTO, UNICAMP; MARCELO F. CARAZZOLLE, UNICAMP; GONÇALO A. G. PEREIRA, UNICAMP; ROGÉRIO MARGIS, UFRGS. |
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Título: |
Identification of novel soybean microRNAs involved in abiotic and biotic stresses. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
BMC Genomics, v. 12, n. 307, jun. 2011. |
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Páginas: |
17 p. |
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DOI: |
10.1186/1471-2164-12-307 |
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Idioma: |
Português |
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Conteúdo: |
Background: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses. MenosBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also di... Mostrar Tudo |
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Thesagro: |
Deficiência hídrica; Gene marcador; Soja. |
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Thesaurus NAL: |
Gene expression; Soil water deficit; Soybeans. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51756/1/BMC-2011.pdf
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Marc: |
LEADER 03216naa a2200385 a 4500
001 1911802
005 2013-04-03
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1186/1471-2164-12-307$2DOI
100 1 $aKULCHESKI, F. R.
245 $aIdentification of novel soybean microRNAs involved in abiotic and biotic stresses.
260 $c2011
300 $a17 p.
520 $aBackground: Small RNAs (19-24 nt) are key regulators of gene expression that guide both transcriptional and posttranscriptional silencing mechanisms in eukaryotes. Current studies have demonstrated that microRNAs (miRNAs) act in several plant pathways associated with tissue proliferation, differentiation, and development and in response to abiotic and biotic stresses. In order to identify new miRNAs in soybean and to verify those that are possibly water deficit and rust-stress regulated, eight libraries of small RNAs were constructed and submitted to Solexa sequencing. Results: The libraries were developed from drought-sensitive and tolerant seedlings and rust-susceptible and resistant soybeans with or without stressors. Sequencing the library and subsequent analyses detected 256 miRNAs. From this total, we identified 24 families of novel miRNAs that had not been reported before, six families of conserved miRNAs that exist in other plants species, and 22 families previously reported in soybean. We also observed the presence of several isomiRNAs during our analyses. To validate novel miRNAs, we performed RT-qPCR across the eight different libraries. Among the 11 miRNAs analyzed, all showed different expression profiles during biotic and abiotic stresses to soybean. The majority of miRNAs were up-regulated during water deficit stress in the sensitive plants. However, for the tolerant genotype, most of the miRNAs were down regulated. The pattern of miRNAs expression was also different for the distinct genotypes submitted to the pathogen stress. Most miRNAs were down regulated during the fungus infection in the susceptible genotype; however, in the resistant genotype, most miRNAs did not vary during rust attack. A prediction of the putative targets was carried out for conserved and novel miRNAs families. Conclusions: Validation of our results with quantitative RT-qPCR revealed that Solexa sequencing is a powerful tool for miRNA discovery. The identification of differentially expressed plant miRNAs provides molecular evidence for the possible involvement of miRNAs in the process of water deficit- and rust-stress responses.
650 $aGene expression
650 $aSoil water deficit
650 $aSoybeans
650 $aDeficiência hídrica
650 $aGene marcador
650 $aSoja
700 1 $aOLIVEIRA, L. F. V.
700 1 $aMOLINA, L. G.
700 1 $aALMERAO, M. P.
700 1 $aRODRIGUES, F. A.
700 1 $aMARCOLINO, J.
700 1 $aBARBOSA, J. F.
700 1 $aSTOLF-MOREIRA, R.
700 1 $aNEPOMUCENO, A. L.
700 1 $aMARCELINO-GUIMARÃES, F. C.
700 1 $aABDELNOOR, R. V.
700 1 $aNASCIMENTO, L. C.
700 1 $aCARAZZOLLE, M. F.
700 1 $aPEREIRA, G. A. G.
700 1 $aMARGIS, R.
773 $tBMC Genomics$gv. 12, n. 307, jun. 2011.
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